Zbornik - Društvo genetičara Srbije

116 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Usm-27
Subotica, 30. novembar - 4. decembar 2004.
NANOMOLEKULSKI PREKIDAÈI I STARENJE SEMENA HIBRIDA
KUKURUZA I NJIHOVIH RODITELJSKIH KOMPONENTI
V. Dragièeviæ 1 , S. Sredojeviæ 1 , M. Spasiæ 2 , M. Srebriæ 1 , M. Ivanoviæ 3 i M. Vrviæ 2
1
Institut za kukruz «Zemun Polje», Zemun Polje
2
Hemijski fakultet, Beograd
3
Institut za ratarstvo i povrtarstvo, Novi Sad
Od presudnog znaèaja za odranje ivotnih funkcija ivih sistema je postojanje
nanomolekulskih prekidaèa koji modulišu njihov redoks ekvilibrijum. Bazu tog
mehanizma èine jedinjenja tipa proteinskih sulfhidrila (PSH) i glutationa (GSH), kao i
njihovi oksidovani oblici (PSSP i GSSG). Praæenjem promena u koncentracijama
navedenih jedinjenja tokom ubrzanog starenja semena dva hibrida kukuruza iz grupe
zrenja 500: ZP SC 580-H1 i ZP SC 504su-H2 i njihovih roditeljskih komponenti: ZP PL
175 ( )-L1, ZP PL 188 ( )-L2 i ZP PL 51 ( )-L3, ZP PL 67 ( )-L4, konstatovano je
postojanje dva tipa nanomolekulskih prekidaèa: tip I, koji paralelno ukljuèuje oksidaciju
GSH i PSH i tip II koji u kljuèuje oksidaciju GSH, uz poveæanje nivoa PSH. Potrebno je
naglasiti da su kod svih ispitivanih linija i hibrida bila prisutna oba tipa prekidaèa, s tim
što je dominantnost svakog od njih bila karakteristika pojedinog hibrida. Kod H1 bio je
prisutan pad nivoa GSH i PSH, uz poveæanje GSSG, što je karakteristièno za prekidaè
tipa I, dok je kod H2 bio prisutan pad nivoa GSH, uz poveæanje GSSG i PSH, a što je
karakteristièno za prekidaè tipa II. Kod L1 i L2 bio je dominantan tip I prekidaèa, kao
kod H1, dok je kod L3 bio dominantan tip II prekidaèa, kao kod H2. Izneta razmatranja bi
mogla da poslue za klasifikaciju genotipova prema dominantnom nanoprekidaèu, uz
otvaranje pitanja njihovog uticaja na mehanizme otpornosti.
THE NANOMOLECULAR SWITCHES AND AGEING OF MAIZE HYBRID
SEEDS AND THEIR INBREDS
The vital property of living systems conditioning is connected to existence of nanomolecular
switches, via modulation of redox equilibrium. The noted mechanism is based on
presence of sulfhydryl proteins (PSH) and glutathione (GSH), together with their oxidised
forms (PSSP and GSSG). The seeds of two maize hybrids (FAO 500): ZP SC 580 -
H1 and ZP SC 504su - H2 and their inbreds: ZP PL 175 ( )-L1, ZP PL 188 ( )-L2 and ZP
PL 51 ( )-L3, ZP PL 67 ( )-L4, were subjected to accelerated ageing treatment up to 12
days. The changes in concentration of related compounds are evidence for existence of
two types of nanomolecular switches: type I – includes oxidation of GSH and PSH and
type II – includes oxidation of GSH, with increase of PSH level. It is important to underline
that all examined hybrids and their inbreds had both types of nanoswitches, while genotypes
differ by domination of one of them. There is decrease in GSH and PSH and
raise in GSSG content in H1, what is characteristic for type I of switch, whereas the
lower GSH, increased PSH and GSSG content in H2 is typical for type II. The dominance
of type I of switch was obtained in L1 and L2, as in H1, but the domination of type II was
achieved in L3, correspondingly to H2. The noted sentences could be used for classification
of genotypes in mode of dominated nanoswitch with opening of question of its influence
on resistance mechanisms.