Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
Zbornik - Društvo genetičara Srbije
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116 ZBORNIK ABSTRAKATA III KONGRESA GENETIÈARA SRBIJE IV-Usm-27<br />
Subotica, 30. novembar - 4. decembar 2004.<br />
NANOMOLEKULSKI PREKIDAÈI I STARENJE SEMENA HIBRIDA<br />
KUKURUZA I NJIHOVIH RODITELJSKIH KOMPONENTI<br />
V. Dragièeviæ 1 , S. Sredojeviæ 1 , M. Spasiæ 2 , M. Srebriæ 1 , M. Ivanoviæ 3 i M. Vrviæ 2<br />
1<br />
Institut za kukruz «Zemun Polje», Zemun Polje<br />
2<br />
Hemijski fakultet, Beograd<br />
3<br />
Institut za ratarstvo i povrtarstvo, Novi Sad<br />
Od presudnog znaèaja za odranje ivotnih funkcija ivih sistema je postojanje<br />
nanomolekulskih prekidaèa koji modulišu njihov redoks ekvilibrijum. Bazu tog<br />
mehanizma èine jedinjenja tipa proteinskih sulfhidrila (PSH) i glutationa (GSH), kao i<br />
njihovi oksidovani oblici (PSSP i GSSG). Praæenjem promena u koncentracijama<br />
navedenih jedinjenja tokom ubrzanog starenja semena dva hibrida kukuruza iz grupe<br />
zrenja 500: ZP SC 580-H1 i ZP SC 504su-H2 i njihovih roditeljskih komponenti: ZP PL<br />
175 ( )-L1, ZP PL 188 ( )-L2 i ZP PL 51 ( )-L3, ZP PL 67 ( )-L4, konstatovano je<br />
postojanje dva tipa nanomolekulskih prekidaèa: tip I, koji paralelno ukljuèuje oksidaciju<br />
GSH i PSH i tip II koji u kljuèuje oksidaciju GSH, uz poveæanje nivoa PSH. Potrebno je<br />
naglasiti da su kod svih ispitivanih linija i hibrida bila prisutna oba tipa prekidaèa, s tim<br />
što je dominantnost svakog od njih bila karakteristika pojedinog hibrida. Kod H1 bio je<br />
prisutan pad nivoa GSH i PSH, uz poveæanje GSSG, što je karakteristièno za prekidaè<br />
tipa I, dok je kod H2 bio prisutan pad nivoa GSH, uz poveæanje GSSG i PSH, a što je<br />
karakteristièno za prekidaè tipa II. Kod L1 i L2 bio je dominantan tip I prekidaèa, kao<br />
kod H1, dok je kod L3 bio dominantan tip II prekidaèa, kao kod H2. Izneta razmatranja bi<br />
mogla da poslue za klasifikaciju genotipova prema dominantnom nanoprekidaèu, uz<br />
otvaranje pitanja njihovog uticaja na mehanizme otpornosti.<br />
THE NANOMOLECULAR SWITCHES AND AGEING OF MAIZE HYBRID<br />
SEEDS AND THEIR INBREDS<br />
The vital property of living systems conditioning is connected to existence of nanomolecular<br />
switches, via modulation of redox equilibrium. The noted mechanism is based on<br />
presence of sulfhydryl proteins (PSH) and glutathione (GSH), together with their oxidised<br />
forms (PSSP and GSSG). The seeds of two maize hybrids (FAO 500): ZP SC 580 -<br />
H1 and ZP SC 504su - H2 and their inbreds: ZP PL 175 ( )-L1, ZP PL 188 ( )-L2 and ZP<br />
PL 51 ( )-L3, ZP PL 67 ( )-L4, were subjected to accelerated ageing treatment up to 12<br />
days. The changes in concentration of related compounds are evidence for existence of<br />
two types of nanomolecular switches: type I – includes oxidation of GSH and PSH and<br />
type II – includes oxidation of GSH, with increase of PSH level. It is important to underline<br />
that all examined hybrids and their inbreds had both types of nanoswitches, while genotypes<br />
differ by domination of one of them. There is decrease in GSH and PSH and<br />
raise in GSSG content in H1, what is characteristic for type I of switch, whereas the<br />
lower GSH, increased PSH and GSSG content in H2 is typical for type II. The dominance<br />
of type I of switch was obtained in L1 and L2, as in H1, but the domination of type II was<br />
achieved in L3, correspondingly to H2. The noted sentences could be used for classification<br />
of genotypes in mode of dominated nanoswitch with opening of question of its influence<br />
on resistance mechanisms.