CS18 - Chromatographic Specialties
CS18 - Chromatographic Specialties
CS18 - Chromatographic Specialties
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CHROMATOGRAPHIC<br />
SPECIALTIES INC.<br />
www.chromspec.com<br />
HPLC COLUMNS BY USP L-CODE Technical Note <strong>CS18</strong><br />
The United States Pharmacopeia (USP) maintains a list of codes to specify generic GC or HPLC stationary phases. The phase<br />
definitions are broad, and many columns fit into the more popular categories. Columns within a category will have subtle to not-sosubtle<br />
differences in performance. For instance, a sub-2µm double endcapped C18 on ultra-pure type B spherical silica and a 10<br />
µm non-endcapped C18 on original type A irregular silica are both L1 phases. They will not produce the same results.<br />
The following list provides an overview of the columns that CSI can provide for each USP code. This is not an exhaustive list.<br />
These are columns recommended for new method development, along with some columns that we have recently gained access to<br />
for older methods.<br />
USP L-classes are very broad. It is still vital to match your column to your analyte and analysis conditions.<br />
For any questions please contact the CSI Technical Support Staff, who are always happy to discuss<br />
your analyte and mobile phase to help select the best match from our extensive line of columns.<br />
Phase USP Specifications Suggested Column<br />
L1 Octadecyl silane chemically bonded to porous silica or NUCLEODUR ® : C18ec, Gravity C18, ISIS, Pyramid<br />
ceramic micro-particles, 1.5 - 10 µm diameter, or a monolithic silica rod NUCLEOSIL ® : C18, C18 AB, C18 HD, C18 Nautilus, C18 MPN, C18 PAH, C18 PPN<br />
Allure ® : C18, Aqueous C18<br />
Pinnacle ® DB: C18, X3 C18, Aqueous C18<br />
Pinnacle II ® C18, Ultra II C18, Viva C18<br />
YMC UltraHT Pro: C18, Hydrosphere C18<br />
YMC-Pack Pro: C18, C18 RS, Hydrosphere C18<br />
YMC-Pack: ODS-A, ODS-AM, ODS-AQ, ODS-AL<br />
J’sphere: ODS-H80, ODS-M80, ODS-L80<br />
Genesis ® C18, APEX C18<br />
Alltima HP C18, C18 EPS, C18 HiLoad, C18 Amide<br />
Adsorbosphere C18, C18 HS, C18-B and C18 UHS<br />
Allsphere ODS-1, ODS-2<br />
Brava C18 BDS, C18 ODS<br />
Prevail Select C18, C18<br />
Allsphere ODS-1, ODS-2, ProSphere <br />
Platinum C18, EPS C18<br />
Grace ® VisionHT C18, C18-B, C18-P, C18-HL<br />
Grom Sil, Vydac ® TP<br />
BlueOrchid C18<br />
Hx-Sil C18, OroSil , OroStar , Selectra ®<br />
L3 Porous silica particles, 3-10 µm diameter, or a monolithic silica rod NUCLEOSIL ® , NUCLEODUR ®<br />
Allure ® Silica, Pinnacle ® DB Silica, Pinnacle II ® Silica, Ultra II Silica, Viva Silica<br />
YMC-Pack SIL<br />
Alltima HP, Prevail , Platinum , Econosphere <br />
Adsorbosphere , Allsphere Apollo , Brava <br />
Genesis ® Silica, APEX Silica<br />
L7 Octylsilane chemically bonded to totally porous silica particles, NUCLEODUR ® : C8 ec, Gravity C8<br />
1.5-10 µm diameter, or a monolithic silica rod NUCLEOSIL ® : C8 ec, C8, C8 HD<br />
Pinnacle DB C8, Pinnacle ® II C8, Ultra C8, Viva C8<br />
YMC-Pack Pro C8, YMC-Pack C8<br />
Adsorbosphere , Allsphere , Alltima , Apollo <br />
Brava , Econosil , Grom Sil, Platinum <br />
Prevail , Vydac 208TP-C8<br />
Genesis ® C8, APEX C8, Hx-Sil C8<br />
OroSil <br />
L8 An essentially monomolecular layer of aminopropylsilane NUCLEODUR ® NH2 / NH2-RP, NUCLEOSIL ® NH2 / NH2-RP<br />
chemically bonded to totally porous silica gel support, 3-10 µm diameter APEX NH2, Pinnacle ® II Amino, Ultra Amino<br />
YMC-Pack NH2<br />
Adsorbopshere , Allsphere , Alltima - Amino columns<br />
Brava , Econosphere , Grom Sil- Amino columns
Phase USP Specifications Suggested Column<br />
L9 Totally porous silica gel with chemically bonded, strong NUCLEOSIL ® 100 SA<br />
cation-exchange coating, 3-10 µm diameter.<br />
L10 Nitrile groups chemically bonded to porous silica particles, NUCLEODUR ® CN / CN-RP, NUCLEOSIL ® CN / CN-RP<br />
3-10 µm diameter. Pinnacle ® DB Cyano, Pinnacle ® II Cyano, Ultra Cyano, Allure ® Basix<br />
YMC-Pack CN<br />
Adsorbosphere CN, Allsphere CN<br />
Alltima HP CN<br />
Brava -CN, Cyano BDS<br />
Econosphere CN, Platinum CN, Prevail CN<br />
BlueOrchid Cyano<br />
L11 Phenyl groups chemically bonded to porous silica particles, NUCLEOSIL ® C6H5, NUCLEOSIL ® C6H5 ec, Nucleodur ® Sphinx RP<br />
1.5-10 µm diameter. Pinnacle ® DB Phenyl, Pinnacle ® II Phenyl, Ultra II Biphenyl<br />
Allure ® Biphenyl, Pinnacle ® DB Biphenyl, Pinnacle ® II Biphenyl, Viva Biphenyl<br />
YMC-Pack Phenyl<br />
Adsorbosphere Phenyl, Allsphere Phenyl<br />
Alltima Phenyl, Apollo Phenyl, Brava Phenyl<br />
Grom Sil- Phenyl-1 FE<br />
Grom Sil- Phenyl-2 CP<br />
Grom Sil- Phenyl-3 PE<br />
Platinum Phenyl, Prevail Phenyl<br />
BlueOrchid Phenyl<br />
L13 Trimethylsilane chemically bonded to porous silica particles, Adsorbosphere XL C1<br />
3-10 µm diameter. Allsphere C1<br />
Grom Sil-TMS-1 ST<br />
Grom Sil-TMS-2 CP<br />
YMC-Pack TMS<br />
L14 Silica gel having a chemically bonded, strongly basic NUCLEOSIL ® 100 SB<br />
quaternary ammonium anion-exchange coating, 5-10 µm diameter. Partisil 10 SAX<br />
L15 3-10 µm hexyl bonded porous silica Allsphere C6<br />
L16 Dimethylsilane chemically bonded to porous silica particles, NUCLEOSIL ® C2<br />
5-10 µm diameter.<br />
L17 Strong cation exchange resin consisting of sulfonated cross-linked Hamilton HC-75 H + , PRP ® -X200, PRP ® -X400<br />
styrene-divinylbenzene copolymer in the hydrogen form, NUCLEOGEL ® ION 300 OA, SUGAR 810 H<br />
7-11 µm diameter<br />
L18 Amino and cyano groups chemically bonded to porous silica particles, Partisil PAC<br />
3-10 µm diameter<br />
L19 Strong cation exchange resin consisting of sulfonated cross-linked Hamilton HC-75 Ca 2+<br />
styrene-divinylbenzene copolymer in the calcium form, NUCLEOGEL ® SUGAR Ca, SUGAR 810 Ca<br />
about 9 µm diameter<br />
L20 Dihydroxypropane groups chemically bonded to porous silica particles, NUCLEOSIL ® OH<br />
5-10 µm diameter YMC-Pack Diol-NP<br />
Grom Sil- Diol<br />
APEX Diol<br />
L21 A rigid spherical styrene-divinylbenzene copolymer, 5-10 µm diameter Hamilton PRP ® -1, PRP ® -3<br />
NUCLEOGEL ® RP<br />
L22 A cation-exchange resin made of porous polystyrene gel with sulfonic NUCLEOGEL ® SCX<br />
acid groups, about 10 µm size<br />
L23 An anion-exchange resin made of porous polymethacrylate or Hamilton PRP ® -X500<br />
polyacrylate gel with quaternary ammonium groups, about 10 µm size NUCLEOGEL ® SAX<br />
L26 Butyl silane chemically bonded to totally porous silica particles, NUCLEOSIL ® C4, C4 MPN<br />
3-10 µm diameter Ultra C4, Viva C4<br />
YMC-Pack Pro C4, YMC-Pack C4<br />
Grom Sil- Butyl-2 FE<br />
Grom Sil- Butyl-1 ST<br />
Vydac ® 214TP C4<br />
Vydac ® MS C4<br />
Genesis ® C4<br />
L30 Ethyl silane chemically bonded to a totally porous silica particle, APEX Prepsil C2<br />
3-10 µm diameter<br />
L32 A chiral ligand-exchange resin packing · L-proline copper complex NUCLEOSIL ® CHIRAL-1<br />
covalently bonded to irregular shaped silica particles, 5-10 µm diameter
Phase USP Specifications Suggested Column<br />
L34 Strong cation exchange resin consisting of sulfonated cross-linked Hamilton HC-75 Pb 2+<br />
styrene-divinylbenzene copolymer in the lead form, NUCLEOGEL ® SUGAR Pb<br />
about 9 µm diameter<br />
L36 A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded NUCLEOSIL ® CHIRAL-3<br />
to 5 µm aminopropyl silica<br />
L40 Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica NUCLEOCEL DELTA<br />
particles, 5-20 µm diameter<br />
L43 Pentafluorphenyl groups chemically bonded to silica particles Pinnacle ® DB PFP Propyl, Ultra II PFP, Allure ® PFP Propyl, Viva PFP Propyl<br />
by a propyl spacer, 5-10 µm diameter<br />
L45 Beta clyclodextrin bonded to porous silica particles 5-10 µm diameter ChiraDex Chiral<br />
NUCLEODEX B-OH<br />
YMC CHIRAL ß-CD BR<br />
L51 Amylose tris-3,5-dimethylphenylcarbamate coated porous, NUCLEOCEL ALPHA<br />
spherical silica particles, 5-10 µm diameter<br />
L57 A chiral-recognition protein, ovomucoid, chemically bonded to silica Ultron ES-OVM<br />
particles, about 5 µm diameter, with a pore size of 120Å<br />
L58 Strong cation-exchange resin consisting of sulfonated cross-linked NUCLEOGEL ® SUGAR Na<br />
PS/DVB copolymer in the Na form, 6-30 µm diameter<br />
L60 Spherical porous silica gel, 3 or 5 µm diameter, the surface of which NUCLEOSIL ® C18 Nautilus<br />
has been covalently modified with alkyl amide groups and endcapped<br />
For further information, please contact our Technical Support Team at<br />
1-800-267-8103 or email tech@chromspec.com.<br />
CHROMATOGRAPHIC SPECIALTIES INC.<br />
P.O. Bag 1150, 300 Laurier Blvd., Brockville, ON K6V 5W1<br />
Toll Free: 800-267-8103 • Phone: 613-342-4678 • Fax: 613-342-1144<br />
www.chromspec.com • tech@chromspec.com