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All statistics were performed using Minitab version 10.0, except for the Kolmogorov- Smirnov test which was carried out by hand using the formula given by Zar (1984) and the tables given by Smirnov (1948). Multivariate analyses were carried out on the faunal data to assess (dis)similarities between community assemblages. All multivariate analyses were performed using the PRIMER (Plymouth Routines In Multivariate Ecological Research) package, version 4.0 (see Warwick and Clarke, 1994). Dendrograms were produced by hierarchical agglomerative clustering with group-average linking from the Bray-Curtis similarity matrices. The raw community data were square-root transformed, this was chosen a priori as a compromise between no transformation in which different community assemblages may result from the variability in the most common taxa, and a strong transformation, such as -JAI or log(x+1), in which the rarer species have very strong influences on community (dis)similarities (Warwick and Clarke, 1994). Non-metric Multi-Dimensional Scaling (or MDS) was carried out from which an ordination plot was produced. In ordination plots, the relative distances apart of the samples reflect relative similarity in species composition. Since the MDS ordination represents a multi-dimensional ordination in 2 dimensions, each algorithm has an associated stress value, the influence of which on the reliability of ordination plots is discussed by Warwick and Clarke (1994). The final MDS ordination in each analysis was that with the lowest associated stress value out of 9 iterations. The MDS procedure was repeated 10 times for each analysis to minimise the chance of producing MDS plots with only 'local minimum' stress functions (Warwick and Clarke, 1994), i.e., increasing the number of starting configurations of points in the ordination plots increases the chance of producing the most optimum MDS plot. Testing for significance between patch and non-patch communities was performed using a One-way ANOSIM test (analysis of similarities) in which the null hypothesis (Ho) in each case was that there were no significant community differences between the two plot types. The ANOSIIVI test can be regarded as a non-parametric equivalent of the MANOVA test (e.g., Mardia et al., 1979) in which few, if any, assumptions about the data are made. Benthic community data are usually far from normally 139

distributed (Clarke, 1993) and, therefore, a non-parametric test is usually more suitable. However, no corrections are made for multiple pairwise testing (Warwick and Clarke, 1994), consequently, more emphasis should be placed on the value of R, the test statistic, rather than the p value. The test statistic R will always be between 0 and 1; if R-,-. 1 all replicates within sites are more similar to each other than any other replicates from different sites while if R---0 similarities between and within sites will be the same on average. As with standard univariate tests, it is possible for R to be significantly different from zero yet relatively small if there are many replicates for each site. The ANOSIM test is more reliable for indicating treatment differences than the MDS plot since it works on the full similarity matrix rather than the approximation to it in 2-dimensions (Warwick and Clarke, 1994). 140

distributed (Clarke, 1993) and, therefore, a non-parametric test is usually more<br />

suitable. However, no corrections are made for multiple pairwise testing (Warwick<br />

and Clarke, 1994), consequently, more emphasis should be placed on the value of R,<br />

the test statistic, rather than the p value. The test statistic R will always be between 0<br />

and 1; if R-,-. 1 all replicates within sites are more similar to each other than any other<br />

replicates from different sites while if R---0 similarities between and within sites will<br />

be the same on average. As with standard univariate tests, it is possible for R to be<br />

significantly different from zero yet relatively small if there are many replicates for<br />

each site. The ANOSIM test is more reliable for indicating treatment differences than<br />

the MDS plot since it works on the full similarity matrix rather than the approximation<br />

to it in 2-dimensions (Warwick and Clarke, 1994).<br />

140

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