THESIS APPROVAL

uptake of naphthalene into fish, and six sampling times (2, 4, 6, 8, 10 and 12 h) were
provided to evaluate the amount of naphthalene transferred to fish through the worm
diet. All experimental units were carried out with three replicates.
Table 12 Experimental design used in the trophic transfer experiment.
Experimental
Units
Treatments
T-A Naphthalenecontaminated
T-B Naphthalene-
contaminated
Worm diet Feeding time
(h)
Exposure period
(h)
0 2
4
6
8
10
12
0, 6 2
4
6
8
10
12
Controls
C-A Control worm 0 2
4
6
8
10
C-B Control worm 0, 6 2
4
6
8
10
12
2) Test Organisms
12
Number of fish
(ind/beaker)
Fish used in the trophic transfer experiment were of same age and
size conditions in order to reduce any bias during the experiment. The fingerling,
17 w old and 7 cm in length, were purchased from Pathumthani Inland Fisheries
Research and Development Center, Pathumthani Province. Three individual fish were
sampled for measuring weight and then kept for background analysis of naphthalene
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
55