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especially abundant in this area (Ngamprayad, 1999). The predominant species in this system was the tubificid oligochaete Limnodrilus hoffmeisteri, and the abundance could reach densities greater than 10,000 ind/m 2 (Kanchana-Aksorn and Petpiroon, 2006). This finding suggests that L. hoffmeisteri is tolerant to multiple stresses including high organic sediment, low DO water and PAHs contamination. 38
1. Instruments MATERIALS AND METHODS Materials 1.1 Stereo microscope (Nicon, SMZ-2B, Japan) 1.2 Compound microscope (Nicon, E400, Japan) 1.3 SEM (Jeol, JSM 5600 LV, Japan) 1.4 Critical point dryer (Hitachi, HCP-2, Japan) 1.5 Ion coater (Eiko, IB-2, Japan) 1.6 Digital camera (Fujifilm, FinePix S9600, Japan) 1.7 Hot air oven (Memmert, ULE 500, Germany) 1.8 Muffle furnace (Vulcan, 3-1750, USA) 1.9 Microwave extraction system (CEM Corporation, MARS-X, USA) 1.10 Rotary evaporator (Buchi, R-205, Switzerland) 1.11 Homogenizer (Kinematica, PT-2100, UK) 1.12 Centrifuge (Jouan, MR 23i, France) 1.13 Magnetic stirrer (HL, MS-115, India) 1.14 HPLC (Shimadzu, LC 2010, Japan) 1.15 C18 column (3.9 mm×15 cm, alphaBond, Alltech, USA) 1.16 Photodiode array detector (Shimadzu, LC-10ADVP, Japan) 1.17 Separatory funnel 1.18 Mortar and pestle 1.19 Stainless sieve (0.5, 0.25 and 0.063 mm mesh, Endecotts, England) 1.20 Analytical balancer (4 digits, Mettler-Toledo, AB 204-S, Switzerland) 1.21 Micrometer (range: 0-75 mm, INSIZE, IS13101, China) 1.22 DO meter (Eutech, Cyberscan DO 6000, Singapore) 39
Page 1 and 2: THESIS APPROVAL GRADUATE SCHOOL, KA
Page 3 and 4: Warucha Kanchana-Aksorn 2009: Use o
Page 5 and 6: TABLE OF CONTENTS Page TABLE OF CON
Page 7 and 8: LIST OF TABLES (Continued) Table Pa
Page 9 and 10: LIST OF TABLES (Continued) Appendix
Page 11 and 12: LIST OF FIGURES Figure Page 1 Aquat
Page 13 and 14: LIST OF FIGURES (Continued) Appendi
Page 15 and 16: USE OF FRESHWATER OLIGOCHAETE (LIMN
Page 17 and 18: At this stage, laboratory study on
Page 19 and 20: LITERATURE REVIEW Methodology of To
Page 21 and 22: through development, such as hatchi
Page 23 and 24: ange of 0.15-1.5 is thus expected p
Page 25 and 26: on microcustaceans, insect larvae a
Page 27 and 28: Figure 4 Budding in oligochaete sho
Page 29 and 30: Thalassodrilus, Clitellio, Adelodri
Page 31 and 32: Figure 7 Tail protrusion and swayin
Page 33 and 34: Figure 8 Chemical structures of six
Page 35 and 36: oil and asphalt may contain several
Page 37 and 38: several PAHs in water and sediment,
Page 39 and 40: 5. Transformation by Microorganisms
Page 41 and 42: et al., 2005), while in shore crabs
Page 43 and 44: 7. Toxic Effects in Aquatic Organis
Page 45 and 46: Table 6 (Continued) PAHs Species LC
Page 47 and 48: their exposure to digestive fluids
Page 49 and 50: during the sediment aging process,
Page 51: Changes in bottom sediment in the l
Page 55 and 56: the technique of mitochondrial 16s
Page 57 and 58: 2. Investigation on the Morphologic
Page 59 and 60: and no worms placed to the sediment
Page 61 and 62: 3.4 Data Analysis All values were r
Page 63 and 64: Table 9). Spiked sediment was subsa
Page 65 and 66: Sediment avoidance in ij = (Σ anim
Page 67 and 68: 3) Test Conditions The bioaccumulat
Page 69 and 70: uptake of naphthalene into fish, an
Page 71 and 72: 4) Exposure Study Trophic transfer
Page 73 and 74: performed with 20 ml extraction flu
Page 75 and 76: RESULTS AND DISCUSSION Results 1. B
Page 77 and 78: Figure 13 Morphological features of
Page 79 and 80: Living style of L. hoffmeisteri was
Page 81 and 82: Figure 17 Feeding behavior of L. ho
Page 83 and 84: worms in this old class whereas a l
Page 85 and 86: No. of ind No. of ind No. of ind No
Page 87 and 88: Figure 23 Adult worm (3.0-3.5 cm) i
Page 89 and 90: 3.4 Relationship between Oligochaet
Page 91 and 92: Table 14 Lethal and sublethal effec
Page 93 and 94: Figure 29 Mortality of L. hoffmeist
Page 95 and 96: ioaccumulation test, respectively (
Page 97 and 98: Concentration (ug/g dwt) 2000 1500
Page 99 and 100: analysis from two-factor ANOVA show
Page 101 and 102: sediment particles and accumulated
Page 103 and 104:
undulatory swimming. Also, the peri
Page 105 and 106:
The existence of L. hoffmeisteri in
Page 107 and 108:
Lumbriculus variegatus ceased at le
Page 109 and 110:
low level of DO had thoroughly occu
Page 111 and 112:
in water, and thus is available for
Page 113 and 114:
(1988) who investigated subacute re
Page 115 and 116:
pollutants in aquatic ecosystems (M
Page 117 and 118:
et al., 1994b), in dosed sediments
Page 119 and 120:
(1997) said that naphthalene is mod
Page 121 and 122:
107 various studies showed that fis
Page 123 and 124:
(Dobroski and Epifano, 1980; Wolfe
Page 125 and 126:
111 experiment, indicated the repro
Page 127 and 128:
Recommendations 1. Limnodrilus hoff
Page 129 and 130:
Anderson, J. W., J. M. Neff, B. A.
Page 131 and 132:
Bender, M. E. and R. J. Huggett. 19
Page 133 and 134:
Broman, D., A. Colmjo and C. Naf. 1
Page 135 and 136:
Collado, R. and R. M. Schmelz. 2001
Page 137 and 138:
Driscoll, K. S. B. and A. E. McElro
Page 139 and 140:
Fromm, P. O. and R. C. Hunter. 1969
Page 141 and 142:
Harrel, R. and S. T. Smith. 2002. M
Page 143 and 144:
James, M. O. 1989. Biotransformatio
Page 145 and 146:
Khan, A. N., D. Kamal, M. M. Mahmud
Page 147 and 148:
Lee, H. 1992. Models, muddles and m
Page 149 and 150:
Lotufo, G. R. 1998. Lethal and subl
Page 151 and 152:
McElroy, A. E., B. W. Tripp, J. W.
Page 153 and 154:
Neff, J. M. 1979. Polycyclic Aromat
Page 155 and 156:
Pearson, W. H. and B. L. Olla. 1980
Page 157 and 158:
143 Reichert, W. L., B-T. LeEberhar
Page 159 and 160:
Sarin, C. 1994. Distribution of Ali
Page 161 and 162:
Suedel, B. C., J. A. Boraczek, R. K
Page 163 and 164:
Watanabe, K. H., H. Lin, H. L. Bart
Page 165 and 166:
Yoshioka, Y and Y. Ose. 1993. A qua
Page 167 and 168:
Appendix A Methods of Preparation a
Page 169 and 170:
Dilution of Spiked Sediment 155 The
Page 171 and 172:
Naphthalene Standard Calibration Cu
Page 173 and 174:
Appendix Figure A2 HPLC chromatogra
Page 175 and 176:
Calculation of Naphthalene Concentr
Page 177 and 178:
Appendix Table B1 Characteristics o
Page 179 and 180:
Appendix Table B5 Length-class of L
Page 181 and 182:
Appendix Table B7 Length-class of L
Page 183 and 184:
Appendix Table B10 Acute toxicity o
Page 185 and 186:
Appendix Table B12 Sublethal toxici
Page 187 and 188:
Appendix Table B13 (Continued) Dura
Page 189 and 190:
Appendix Table B15 Naphthalene in t
Page 191 and 192:
177 Appendix Table B17 Naphthalene
Page 193 and 194:
179 Appendix Table B18 Naphthalene
Page 195 and 196:
Appendix C Data Analysis 181
Page 197 and 198:
Appendix Table C3 Two-factor ANOVA
Page 199 and 200:
Page 201 and 202:
Probit Analysis of Mortality Equati
Page 203 and 204:
Probit Analysis of Autotomy Equatio
Page 205 and 206:
Probit Analysis of Sediment Avoidan
Page 207 and 208:
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