THESIS APPROVAL

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Appendix Table B17 (Continued) Duration Replication Weight (h) a Concentration (g dwt) b Amount (µg/g dwt) c (µg) Control A 2 1 0.821 nd - 2 0.756 nd - 3 0.698 nd - 4 1 0.802 nd - 2 0.763 nd - 3 0.787 nd - 6 1 0.751 nd - 2 0.821 nd - 3 0.819 nd - 8 1 0.772 nd - 2 0.793 nd - 3 0.804 nd - 10 1 0.827 nd - 2 0.814 nd - 3 0.795 nd - 12 1 0.809 nd - 2 0.781 nd - 3 0.792 nd - Note: a = Total weight of fingerling muscle in each test vessel. b = Concentration of naphthalene in the fingerling muscle determined using the technique of HPLC. c = Total amount of naphthalene in the fingerling muscle calculated by multiplying a total weight of fingerling muscle (a) with a concentration of naphthalene determined in each test vessel (b). 178
179 Appendix Table B18 Naphthalene in the fingerling muscle from the study on trophic transfer of naphthalene via the oligochaete diet (T-B). Duration Replication Weight (h) a Concentration (g dwt) b Amount (µg/g dwt) c (µg) Treatment B 2 1 0.821 0.952 0.782 2 0.774 0.875 0.677 3 0.793 0.922 0.731 4 1 0.832 2.183 1.817 2 0.845 2.002 1.692 3 0.769 2.013 1.548 6 1 0.773 12.913 9.982 2 0.805 10.836 8.723 3 0.818 11.231 9.187 8 1 0.834 10.937 9.822 2 0.816 9.870 9.754 3 0.788 10.767 9.484 10 1 0.765 3.177 2.431 2 0.809 2.525 2.043 3 0.837 2.344 1.962 12 1 0.758 9.978 7.564 2 0.772 10.435 8.056 3 0.814 9.512 7.743 Note: a = Total weight of fingerling muscle in each test vessel. b = Concentration of naphthalene in the fingerling muscle determined using the technique of HPLC. c = Total amount of naphthalene in the fingerling muscle calculated by multiplying a total weight of fingerling muscle (a) with a concentration of naphthalene determined in each test vessel (b).
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THESIS APPROVAL GRADUATE SCHOOL, KA
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Warucha Kanchana-Aksorn 2009: Use o
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TABLE OF CONTENTS Page TABLE OF CON
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LIST OF TABLES (Continued) Table Pa
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LIST OF TABLES (Continued) Appendix
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LIST OF FIGURES Figure Page 1 Aquat
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LIST OF FIGURES (Continued) Appendi
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USE OF FRESHWATER OLIGOCHAETE (LIMN
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At this stage, laboratory study on
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LITERATURE REVIEW Methodology of To
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through development, such as hatchi
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ange of 0.15-1.5 is thus expected p
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on microcustaceans, insect larvae a
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Figure 4 Budding in oligochaete sho
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Thalassodrilus, Clitellio, Adelodri
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Figure 7 Tail protrusion and swayin
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Figure 8 Chemical structures of six
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oil and asphalt may contain several
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several PAHs in water and sediment,
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5. Transformation by Microorganisms
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et al., 2005), while in shore crabs
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7. Toxic Effects in Aquatic Organis
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Table 6 (Continued) PAHs Species LC
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their exposure to digestive fluids
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during the sediment aging process,
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Changes in bottom sediment in the l
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1. Instruments MATERIALS AND METHOD
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the technique of mitochondrial 16s
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2. Investigation on the Morphologic
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and no worms placed to the sediment
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3.4 Data Analysis All values were r
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Table 9). Spiked sediment was subsa
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Sediment avoidance in ij = (Σ anim
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3) Test Conditions The bioaccumulat
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uptake of naphthalene into fish, an
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4) Exposure Study Trophic transfer
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performed with 20 ml extraction flu
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RESULTS AND DISCUSSION Results 1. B
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Figure 13 Morphological features of
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Living style of L. hoffmeisteri was
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Figure 17 Feeding behavior of L. ho
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worms in this old class whereas a l
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No. of ind No. of ind No. of ind No
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Figure 23 Adult worm (3.0-3.5 cm) i
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3.4 Relationship between Oligochaet
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Table 14 Lethal and sublethal effec
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Figure 29 Mortality of L. hoffmeist
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ioaccumulation test, respectively (
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Concentration (ug/g dwt) 2000 1500
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analysis from two-factor ANOVA show
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sediment particles and accumulated
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undulatory swimming. Also, the peri
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The existence of L. hoffmeisteri in
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Lumbriculus variegatus ceased at le
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low level of DO had thoroughly occu
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in water, and thus is available for
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(1988) who investigated subacute re
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pollutants in aquatic ecosystems (M
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et al., 1994b), in dosed sediments
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(1997) said that naphthalene is mod
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107 various studies showed that fis
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(Dobroski and Epifano, 1980; Wolfe
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111 experiment, indicated the repro
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Recommendations 1. Limnodrilus hoff
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Anderson, J. W., J. M. Neff, B. A.
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Bender, M. E. and R. J. Huggett. 19
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Broman, D., A. Colmjo and C. Naf. 1
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Collado, R. and R. M. Schmelz. 2001
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Driscoll, K. S. B. and A. E. McElro
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Fromm, P. O. and R. C. Hunter. 1969
Page 141 and 142: Harrel, R. and S. T. Smith. 2002. M
Page 143 and 144: James, M. O. 1989. Biotransformatio
Page 145 and 146: Khan, A. N., D. Kamal, M. M. Mahmud
Page 147 and 148: Lee, H. 1992. Models, muddles and m
Page 149 and 150: Lotufo, G. R. 1998. Lethal and subl
Page 151 and 152: McElroy, A. E., B. W. Tripp, J. W.
Page 153 and 154: Neff, J. M. 1979. Polycyclic Aromat
Page 155 and 156: Pearson, W. H. and B. L. Olla. 1980
Page 157 and 158: 143 Reichert, W. L., B-T. LeEberhar
Page 159 and 160: Sarin, C. 1994. Distribution of Ali
Page 161 and 162: Suedel, B. C., J. A. Boraczek, R. K
Page 163 and 164: Watanabe, K. H., H. Lin, H. L. Bart
Page 165 and 166: Yoshioka, Y and Y. Ose. 1993. A qua
Page 167 and 168: Appendix A Methods of Preparation a
Page 169 and 170: Dilution of Spiked Sediment 155 The
Page 171 and 172: Naphthalene Standard Calibration Cu
Page 173 and 174: Appendix Figure A2 HPLC chromatogra
Page 175 and 176: Calculation of Naphthalene Concentr
Page 177 and 178: Appendix Table B1 Characteristics o
Page 179 and 180: Appendix Table B5 Length-class of L
Page 181 and 182: Appendix Table B7 Length-class of L
Page 183 and 184: Appendix Table B10 Acute toxicity o
Page 185 and 186: Appendix Table B12 Sublethal toxici
Page 187 and 188: Appendix Table B13 (Continued) Dura
Page 189 and 190: Appendix Table B15 Naphthalene in t
Page 191: 177 Appendix Table B17 Naphthalene
Page 195 and 196: Appendix C Data Analysis 181
Page 197 and 198: Appendix Table C3 Two-factor ANOVA
Page 201 and 202: Probit Analysis of Mortality Equati
Page 203 and 204: Probit Analysis of Autotomy Equatio
Page 205 and 206: Probit Analysis of Sediment Avoidan
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