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THESIS APPROVAL

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USE OF FRESHWATER OLIGOCHAETE<br />

(LIMNODRILUS HOFFMEISTERI) IN SEDIMENT BIOASSAY<br />

INTRODUCTION<br />

Lower Chao Phraya River, one of the estuaries in Thailand, has shown the<br />

rapid increase in population growth and coastal development. The report from<br />

Pollution Control Department (2001) revealed that the frequency of low dissolved<br />

oxygen and high organic materials tends to be greater, particularly in the urbanized<br />

and industrialized areas. The sources of pollution mostly come from urban industrial<br />

wastewater, agriculture and domestic sewage disposal. These contribute to the<br />

loading of organic pollutants, such as pesticides, heavy metals and petroleum<br />

hydrocarbons, distributing to the water, and setting in bottom sediment of the estuary<br />

(The Office of Environment Policy and Planning, 2001). The deposition of toxicants<br />

in the sediment constitute one of several factors that cause change to the structure of<br />

benthic communities since several chemicals are persistent and can bioaccumulate in<br />

sediment-ingesting infauna (Suess, 1976).<br />

Polycyclic aromatic hydrocarbons (PAHs) are one of the important organic<br />

xenobiotics found in the bottom sediment of Chao Phraya estuary (Wattayakorn,<br />

2003). Previous report by Nokyoo (1995) showed that the concentration of total<br />

PAHs in sediment were in the range of 0.58-4.71 µg/g dwt. However, these levels<br />

were low in comparison with other major world estuaries (e.g., Mille et al., 2007;<br />

Oros et al., 2007). Sources of PAHs in this area originate from oil pollutions along<br />

coastlines including major spills from freighters, tankers and other vessels going<br />

aground and during emptying of oil contaminated ballast and bilged water. Apart<br />

from these, the normal operation of coastal factories and mills adjacent to estuaries<br />

often leads to oil leakage or seepage into water. The runoff due to rainfall also carries<br />

oil from the highways, city streets and industrial areas into the estuary.<br />

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