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1.3.3 Resistant starch content, starch hydrolysis and glycemic index<br />

Resistant starch content, starch hydrolysis and glycemic index<br />

were examined as described in section 1.2.8 and 1.2.9.<br />

1.3.4 Production yield, color, water activity and moisture content<br />

Production yield, color value, water activity and moisture<br />

content were examined as described in section 1.2.4 and 1.2.5.<br />

1.4 Scaled up production of RS III production from HARS<br />

The scaled up (150 g of rice starch in 850 g distilled water) for RS III<br />

formation was chosen by means of the previous experiment (1.3). The RS III was<br />

produced as described in the above experiment 1.2.1. Comparative study on degree of<br />

hydrolysis, viscosity of the debranched product, degree of syneresis, resistant starch<br />

content, water activity and production yield between small and larger-scale<br />

production of RS III with three replicates of all experiments were performed. Degree<br />

of pullulanase hydrolysis and degree of syneresis were examined as described in<br />

section 1.2.2 and 1.2.3.<br />

1.4.1 Viscosity<br />

The viscosity of solutions was measured at room temperature<br />

(30°C). Before measurement, samples were mixed for 30s before removal of a 1 ml<br />

aliquot for degree of hydrolysis determination and the remained sample was measured<br />

a viscosity. The debranched solutions were placed in 250-mL glass beakers with a 10cm<br />

diameter. Viscosity was measured using a Visco Basic Plus (PoLyvisco,<br />

Kinmatica) with a No. L 3 spindle at a speed of 100 rpm. Viscosity was measured in<br />

cP. Viscosity values were assayed in triplicate.<br />

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