THESIS

Methods
1. Production of resistant starch type III from high amylose rice starch by
enzymatically debranching process
1.1 Physiochemical properties of native high amylose rice starch
1.1.1 Proximate Analysis
Moistures content, protein, carbohydrate, fat, ash and fiber content
(%) of high amylose rice starch (HARS) and RS III were determined followed AOAC
(2002) methodology. The procedures are shown in Appendix A.
1.1.2 Total Starch Content
Total starch content (glucose × 0.9) was estimated in 2 N KOH
pretreated samples by an enzymatic method of AOAC (2002). The procedures are
shown in Appendix A.
1.1.3 Amylose content
Amylose content (%) was determined calorimetrically after iodine
binding (Juliano, 1971), using potato amylose as standard. The procedures are shown
in Appendix A.
1.1.4 Scanning electron microscopy (SEM)
The native high amylose rice starch was dried to less than 4%
moisture content and then deposited on a copper disc and coated with gold. The
specimens were examined by mean of the scanning electron microscope (JM-560LV
model). The sample was imaged at 2,000x and 6,000x magnification (Appendix B).
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