THESIS
THESIS
THESIS
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chains but liberates longer chains which then are used to form resistant starch.<br />
Initially when starch is partially debranched and is allowed to retrograde, imperfect<br />
packing of helices in crystallites might be taking place leaving some tight amorphous<br />
regions which can be digested slowly.<br />
The cooperatively interacting length which gives rise to an ordered chain<br />
structure and the density which results from packing might be playing an important<br />
role in the digestibility of these starches. Therefore if the objective is to have high<br />
amount of resistant starch, then the starch needs to be highly debranched by using<br />
non-waxy starch. Longer and complete debranching times promote formation of<br />
resistant starch. Cooling at 15 o C had greatest reduction in starch digestibility was<br />
observed with the highest amount of resistant starch (Guraya et al. (2001a). This<br />
temperature favours nucleation as well as propagation and maturation of crystals.<br />
Rapidly cooling and storing the debranched starch at 1 o C had lower starch<br />
digestibility.<br />
4. Starch Digestive Enzymes<br />
Although glucose can be absorbed from the small intestine (especially<br />
terminal end of the duodenum and jejunum), but maltose and dextrin generated from<br />
starch hydrolysis cannot. Absorptive epithelial cells which line the intestinal villi<br />
produce a number of other ‘brush border’ enzymes to allow for digestion and<br />
subsequent absorption of such materials (disaccharides derived from other sources).<br />
These enzymes are not released into the lumen of the small intestine but are bound to<br />
the membrane of microvilli (Ronald and Matin, 1992).<br />
Consequently, some carbohydrate occurs at the surface of epithelia cells in the<br />
small intestine rather than the lumen of the gut. Brush border enzymes include<br />
sucrase, converting sucrose to glucose and fructose and lactase converting lactose to<br />
glucose and galactose, but these are not relevant to starch metabolism. A trehalase<br />
(converts trehalose to two glucose molecule) is also present. However, maltase<br />
(converting maltose generated from α-amylase activity to glucose) and isomaltase<br />
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