THESIS

1. Scanning Electron Microscopic Examination
1.1 Apparatus
1.1.1 Scanning Electron Microscope (SEM, JEOL, JSM 6301F,
Japan)
1.1.2 Fine coaster (JFC 1200,JEOL, Japan)
1.1.3 Aluminum specimen stub
1.1.4 Double-sided adhesive tape
1.2 Procedure for sample determination
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Scanning Electron Microscope (SEM) of native rice starch, pullulanase
hydrolysis sample and RS III samples were examined according to Atichokudomchai
et al .,(2000). All the samples were dried to less than 4 moisture content before
examined.
Take a dried sample and sprinkle onto double-sided adhesive tape attached
to the specimen stub. Remove the excess sample. Place in fine coater for gold coating
for 150 seconds. Place the coated sample in sample chamber
in the SEM (Appendix
Figure B 1). Examine at magnification of 2,500 X and 6,000X with the accelerating
voltage of 10 KV.
Appendix Figure B1 Scanning Electron Microscope (SEM) instrument