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een used to produce a sample with linear, low-molecular-weight and recrystallization<br />

polymer chains (Guraya et al. 2001 and Yin et al. 2007). Debranching enzymes<br />

rapidly hydrolyze only α-1,6-glucosidic bonds. This releases a mixture of varied unit<br />

chain length from the amylopectin molecule which in turn facilitated starch<br />

retrogradation. In addition, retrogradation is often enhanced when starch gels are<br />

subjected to freezing and thawing treatments (Tovar et al. 2002). Freezing a starch gel<br />

leads to the formation of ice crystals and thus concentrates the starch in non-ice phase.<br />

Upon thawing, the water can be easily compressed from the network, giving rise to a<br />

phenomenon known as syneresis (Tovar et al. 2002). The recrystallinity of rice starch<br />

was altered by pullulanase debranching and freeze-thaw process. The effects of these<br />

treatments on degree of hydrolysis, degree of syneresis, degree of crystallinity,<br />

physicochemical properties, in vitro starch digestibility and glycemic index were<br />

examined. The optimum condition of RS formation was selected to application in low<br />

glycemic index butter cake product.<br />

The food industry is being challenged to redesign traditional foods for<br />

optimal nutritional value, in response to some population sectors with particular<br />

nutritional necessities and making them as tasty as or better than the original. One<br />

way to achieve a healthy food product is to reduce or to omit some of the glucoseladen<br />

ingredients, especially flour and sugar since, at present, diabetic and obesity is<br />

frequently cited as a serious health problem. At the same time, there is a constant<br />

demand for dietetic foods suitable for diabetics, which may have the same calorievalue<br />

being also sucrose-free since this sugar cannot be metabolized without insulin.<br />

For this reason, it is a challenge to develop food products, which was a cake in this<br />

study that low glycemic index for individuals who are intolerant to glucose by using<br />

RS III as flour replacement and high fructose corn syrup as sucrose replacement.<br />

Many carbohydrate-rich foods have high glycemic indexes (GI.), and certainly are not<br />

good in any substantial quantity for people with diabetes. RS III is broken down and<br />

degrade more slowly, releasing glucose gradually into our blood streams and are said<br />

to have lower glycemic indexes. Fructose is very sweet, is roughly 1.75 times sweeter<br />

than sucrose, and is defined as low glycemic ingredient (Jenkin et al. 2002a). Because<br />

fructose must be changed to glucose in the liver in order to be utilized by the body,<br />

2

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