THESIS

118
Non significant differences (P≥0.05) in moisture content and
water activity were observed among the debranching treatments. The moisture content
and water activity were 8.47 to 9.09 % and 0.326 to 0.510, respectively.
Table 17 showed that Hunter L * , a* and b * values of RS III
samples were affected by degree of hydrolysis. This was due to the increased of
reducing sugar. The reducing sugar reacts with amino compounds in the starch slurry
during hydrolysis and storage. The result indicated that the color of the RS III
formation from 16 hr of pullulanase hydrolysis with 10, 12, 14 and 16 unit/g starch
were more yellow than those of the 0 and 8 unit/ g starch. The RS III from 0 and 8
unit/g starch of pullulanase enzyme hydrolysis showed a comparable level of L*
values (whiteness) a* values (red-green axis) and b* values (yellowness) to that by
the higher enzyme concentration (10, 12, 14 and 16 unit/g starch). A comparison with
the treatments
from higher enzyme concentrations showed a significant increase in
red-green axis
and yellowness.
Table 17 Effect of pullulanase enzyme concentration
(0 to 16 unit/g starch for 16h)
on production yield (PY), moistu re content (MC), water activity (aw) and
color
value of RS III from HARS
Enzyme concentration
(unit/g starch)
PY
(%)
0 84.11 b
8 84.99 ab
10 85.92 a
12 85.99 a
14 85.94 a
16 86.16 a
MC
(%)
Hun ter Color value
aw L* a* b*
8.57 ns 0.505 ns
91.91 a
9.09 0.510 90.11 b
8.82 0.426 90.98 b
8.47
8.85
8.47
0.353
0.328
0.326
9 b
90.42 b
90.7
-0.62 b
-0.68 b
-0.91 b
90.44b -0.41 b
0.73 a
0.78 a
2.96 b
3.72 ab
4.26 a
4.84 a
4.84 a
4.26 a
a, b, c….. Means within the same column with different letters are significantly different
(P≤ 0.05) by Ducan’s New multiple-Rang test (DMRT).
ns = Not significantly different (P≥0.05)