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117<br />

molecular weight ( DP n ∼ 30), and a low molecular weight fraction ( DP n ∼ 5) was<br />

found for the resistant starch type III.<br />

Starch digestibility (% dwb)<br />

100<br />

90<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

20<br />

10<br />

0<br />

0 8 10 12 14 16<br />

Pullulanase concentration (unit/g starch)<br />

Resistant starch Digested starch Total starch<br />

Figure 28 Effect of pullulanase enzyme concentration<br />

(0 to 16 unit/g starch for 16h)<br />

on starch digestibility<br />

(% dwb) of RS III formation from HARS<br />

1.3.5 Physiochemical properties<br />

The effects of enzyme concentration (0 to 16 unit/g starch for<br />

16hr) on the physiochemical properties of debrached rice starch paste preheated at<br />

121°C for 30 min showed in Table 17. Production yield (%) is the total weight of the<br />

dry RS III fractions divided by the original weight of the rice starch sample. The<br />

production yield, moisture content, and water activity in RS III formation by<br />

debraching process with different enzyme concentrations were 84.61 to 86.16%. The<br />

slight decreasing in production yield for the lower enzyme concentration<br />

(8 to 10<br />

unit/g starch) might<br />

be due to some losses of starch fraction during freeze-thawed<br />

process and vacuum filtrated. However, no significant differences (P

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