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Nevertheless, the former refers to retarded recovery (after the delay between<br />

compressions), the latter concerns instantaneous recovery (immediately after the first<br />

compression, while the probe goes up). Texture determinations were carried out, after<br />

removing the crust, in (40×40×20) mm-sized samples.<br />

2.1.4 Chemical properties<br />

1) Proximate analysis<br />

Moisture, protein, fat and ash content (%) of the butter cake<br />

samples were determined followed AOAC (2002) methodology (Appendix A)<br />

2) In vitro starch digestibility<br />

The effect of the replace 0, 20, 40, 60, 80 and 100% of the<br />

sugar in butter cakes on in vitro starch digestibility was determined as described in<br />

above experiment 1.2.(1). Butter cake samples were defatted before enzymatic starch<br />

digestion using petroleum ether (25 ml/g x 3, 15 min, ambient temperature)<br />

determination. Three replicates of all experiments were performed.<br />

2.1.5 In vitro starch hydrolysis index and glycemic index<br />

In vitro starch hydrolysis index, glycemic index of the replace<br />

0, 20, 40, 60, 80 or 100% of the sugar in butter cakes were determined as described in<br />

the above experiment 1.2.3. The butter cake samples were defatted before enzymatic<br />

starch digestion using petroleum ether (25 ml/g x 3, 15 min, ambient temperature).<br />

Three replicates of all experiments were performed.<br />

2.1.6 Sensory evaluation<br />

Hedonic sensory tests were conducted by 30 untrained<br />

panelists consisting of Department of Home Economic Faculty of Agriculture,<br />

86

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