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2008 Analysis of the Geometry of Drilled Holes in Demolition ...

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The alignment results show that primate β-defens<strong>in</strong>s are highly conserved.<br />

Humans and orangutans have <strong>the</strong> same am<strong>in</strong>o acid sequence but it should be<br />

noted that humans are more susceptible than orangutans to M tuberculosis.<br />

The multiple sequence alignment file was used as <strong>in</strong>put to <strong>the</strong> Phylip<br />

program to construct a phylogram. The highly-parsimonious phylogram implies<br />

that β-defens<strong>in</strong>s <strong>of</strong> primates, particularly, those <strong>in</strong> <strong>the</strong> Family Hom<strong>in</strong>idae, are<br />

coded by homologous genes and are derived from a common ancestor. However,<br />

additional knowledge on <strong>the</strong> natural history <strong>of</strong> β-defens<strong>in</strong>s and analysis <strong>of</strong><br />

statistical methods are needed to make sure that <strong>the</strong> phylogram is evolutionary<br />

sound.<br />

#79<br />

Activity <strong>of</strong> Honey Aga<strong>in</strong>st Bacterial Stra<strong>in</strong>s Isolated<br />

from Acne Vulgaris Wounds<br />

Ramon Gabriel S. Tenefrancia, Arriane Chiara R. Bernardo, Rafaela Lara B. Cion<br />

#80<br />

Use <strong>of</strong> Garcilaria spp. <strong>in</strong> <strong>the</strong> bioremediation <strong>of</strong> eutrophic<br />

aquatic ecosystems<br />

Joel Mari C Villa Jr., Jest<strong>in</strong> C Bernardo, John Paul A Lappay<br />

ABSTRACT<br />

Gracilaria verricosa, a species <strong>of</strong> red algae (Rhodophyta) was used <strong>in</strong> <strong>the</strong><br />

bioremediation <strong>of</strong> eutrophic aquatic ecosystems. This research was conducted to<br />

curb <strong>the</strong> grow<strong>in</strong>g problem <strong>of</strong>, eutrophication, a result <strong>of</strong> <strong>the</strong> <strong>in</strong>creas<strong>in</strong>g amount <strong>of</strong><br />

nutrients <strong>in</strong> <strong>the</strong> aquatic ecosystem.<br />

Before experimentation, coliform test<strong>in</strong>g was conducted for four<br />

consecutive weeks on <strong>the</strong> PSHS pond to ascerta<strong>in</strong> that eutrophication was actually<br />

occurr<strong>in</strong>g. Three equal amounts <strong>of</strong> samples were <strong>the</strong>n placed <strong>in</strong> separate<br />

conta<strong>in</strong>ers and equal amounts <strong>of</strong> Gracilaria verrucosa bought from a local market<br />

were <strong>the</strong>n soaked <strong>in</strong> each conta<strong>in</strong>er. One set-up was placed completely under<br />

sunlight, ano<strong>the</strong>r under a shady area, and <strong>the</strong> last was completely covered to<br />

prevent sunlight from enter<strong>in</strong>g. After seven days, <strong>the</strong> water samples were<br />

subjected to coliform test<strong>in</strong>g.<br />

Initial test<strong>in</strong>g on <strong>the</strong> water source showed that coliform count <strong>in</strong>creased,<br />

confirm<strong>in</strong>g that <strong>the</strong> water source was eutrophic. After <strong>the</strong> treatment with<br />

Gracilaria verrucosa, however, <strong>the</strong> coliform count and heterotrophic plate count<br />

did not decrease for all set-ups.<br />

The effectiveness <strong>of</strong> Gracilaria spp. <strong>in</strong> bioremediat<strong>in</strong>g eutrophic aquatic<br />

ecosystems could not be determ<strong>in</strong>ed due to <strong>the</strong> use <strong>of</strong> fresh water samples,<br />

which may have caused <strong>the</strong> saltwater species, G verrucosa to die.

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