2008 Analysis of the Geometry of Drilled Holes in Demolition ...

zones of inhibition of the extracts. Disks, 20 mm in diameter, were placed on the
petri dishes.
No zones of inhibition were observed from the set-ups. The use of different
concentrations of extract or different extraction method is recommended.
#75
A Comparative Study of the Effects of Crude Cassava
Leaf Extract and Vincristine on Normal White Blood Cells
Juan Miguel F. Romero, Paula Katarina S. Tayo
ABSTRACT
The project aimed to study and compare the effects of crude cassava leaf
extract and Vincristine, a conventionally-used chemotherapeutic drug, on normal
white blood cells. Cassava leaf extract has already been proven to have a
significant degree of cytotoxic effect on cells, but in addition to this it is suspected
to have a component that may provide it with a cancer-cell-specific property.
Crude cassava leaf extract was acquired by soaking cassava leaves in
ethanol overnight. The mixture was then filtered, subjected to a rotary evaporator
to remove excess ethanol, and centrifuged to remove remaining plant particles. A
small sample of the crude extract was mixed with ferric chloride to verify the
presence of cyanide. The confirmatory test indicated a positive result through the
formation of prussian blue.
Normal blood samples were obtained from a normal subject. The
lymphocytes were isolated for treatment using the histopaque technique. These
white blood cells were then cultured and divided into their respective set-ups. Two
treatments had the cells exposed to varying extract concentrations, 10% and
100%, while another two set-ups was tested on a full dosage of Vincristine and on
pure distilled water respectively. Three replicates were made per treatment to
validate the data.
Based on the results and on the statistical test done, the study shows that
the means of white blood cell deaths that occurred per treatment did not vary by
any significant degree. Thus, this concludes that neither the 10% nor 100% crude
cassava leaf extract showed the cancer-specific property that they were expected
to have.