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2008 Analysis of the Geometry of Drilled Holes in Demolition ...

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applications are many and various, but <strong>the</strong>se prote<strong>in</strong>s are costly and difficult to<br />

procure. Sources from nature are still needed to provide a model for future<br />

syn<strong>the</strong>sis <strong>of</strong> fluorescent prote<strong>in</strong>s with specific characteristics.<br />

This research thus aimed to screen fluorescent prote<strong>in</strong>s from locallyavailable<br />

mar<strong>in</strong>e animals. Biolum<strong>in</strong>escent ech<strong>in</strong>oderms, purple heart urch<strong>in</strong><br />

(astropyga radiata), fire urch<strong>in</strong> (As<strong>the</strong>nosoma varium) and fea<strong>the</strong>r star species<br />

(Comanth<strong>in</strong>a schlegelii), were collected from Mab<strong>in</strong>i, Batangas, and were screened<br />

for fluorescence. Upon exposure to UV light from a Woods Lamps, only<br />

Comanth<strong>in</strong>a schegelii emitted green fluorescence, characteristically distributed <strong>in</strong><br />

its fea<strong>the</strong>r tips. Crude prote<strong>in</strong> extraction as done for all three, but only <strong>the</strong> C<br />

schlegelii extracts were analyzed under <strong>the</strong> fluorescence microscope. Three filters<br />

(DAPI, FITC, and Texas Red) were used to screen for blue, green, and red<br />

emissions).<br />

Comanth<strong>in</strong>a schlegelii extracts proved positive for <strong>the</strong> wavelengths blue,<br />

green and red. However, results also showed colors such as magenta, purple,<br />

and yellow <strong>in</strong> <strong>the</strong> composite view. With setup specifications permitt<strong>in</strong>g only blue,<br />

green, and red emissions, colors from o<strong>the</strong>r wavelengths are deduced as overlaps<br />

probably result<strong>in</strong>g from ei<strong>the</strong>r <strong>the</strong> unverified capability <strong>of</strong> <strong>the</strong> fluorescent prote<strong>in</strong>s<br />

conta<strong>in</strong>ed <strong>in</strong> <strong>the</strong> extracts to be excited at a very wide rang <strong>of</strong> wavelength, or from<br />

<strong>the</strong> overlap <strong>of</strong> two or more fluorescent prote<strong>in</strong>s.<br />

#43<br />

Production <strong>of</strong> Nutrient-Boosted Granola Bars Us<strong>in</strong>g Locally<br />

Available Ingredients<br />

Neil Anthony A. Esguerra, Marcgene Gregandy P. Servito, Kim Arv<strong>in</strong> S. Silvoza<br />

ABSTRACT<br />

The project aimed to produce nutrient-boosted granola bars us<strong>in</strong>g local<br />

<strong>in</strong>gredients to come up with a cheap and nutritious alternative to <strong>the</strong> commercial<br />

granola bars. The <strong>in</strong>itial granola bar recipe was a comb<strong>in</strong>ation <strong>of</strong> malunggay,<br />

yacon, gelat<strong>in</strong> and sticky rice. This <strong>in</strong>itial set-up resulted <strong>in</strong>to a liquid granola<br />

<strong>in</strong>stead <strong>of</strong> <strong>the</strong> normal solid form <strong>of</strong> granola bars. Fur<strong>the</strong>r modification <strong>of</strong> <strong>the</strong> recipe<br />

succeeded <strong>in</strong> mak<strong>in</strong>g a compact and more comparable granola bar by add<strong>in</strong>g<br />

carrots, remov<strong>in</strong>g gelat<strong>in</strong> and replac<strong>in</strong>g sticky rice with oats. Both <strong>the</strong> produced<br />

bars and commercial bars were subjected to sensory evaluation tests which<br />

<strong>in</strong>clude appearance, smell and taste tests. Sign test is used to analyze statistically<br />

<strong>the</strong> result <strong>of</strong> <strong>the</strong> evaluation tests. The shelf life and nutrient content <strong>of</strong> <strong>the</strong><br />

produced granola bar are be<strong>in</strong>g measured as well but are still be<strong>in</strong>g conducted.<br />

In conclusion, it is feasible to produce a homemade granola bar which is a<br />

lot cheaper than <strong>the</strong> commercially-available bars based on computation <strong>of</strong> costs <strong>of</strong><br />

each <strong>in</strong>gredient used. However, <strong>the</strong> statistical analysis done <strong>in</strong> November 21, 2007<br />

showed that <strong>the</strong>re is still a significant difference between <strong>the</strong> produced granola<br />

bar and <strong>the</strong> commercially-available bars <strong>in</strong> terms <strong>of</strong> smell, taste and appearance.

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