SynArfGEF is a guanine nucleotide exchange ... - Pharmacy Eprints

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Abbreviations Arf, ADP ribosylation factor AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate BRAG, brefeldin A-resistant Arf-GEF DAPI, 4', 6-diamidino-2-phenylindole dihydrochloride DGC, dystrophin-associated glycoprotein complex DIV, day in vitro GABA, γ-aminobutyric acid GABAAR, GABAA receptor GAP, GTPase-activating protein GEF, guanine nucleotide exchange factor GGA1, Golgi-localizing, γ-adaptin ear homology domain, Arf-binding protein 1 GST, glutathione S- transferase HA, hemagglutinin IRSP, insulin receptor tyrosine kinase substrate of 53 kDa MAGI, membrane-associated guanylate kinase with inverted orientation MBP, maltose-binding protein Mena/VASP, mammalian enabled/vasodilator stimulated phosphoprotein NMDA, N-methyl-D- aspartate PB, phosphate buffer PBS, phosphate-buffered saline PDZ, PSD-95/Discs large/Zona occludens 1 PSD, postsynaptic density PVDF, polyvinyl difluoride SDS, sodium dodecyl sulfate SDS-PAGE, SDS-polyacrylamide gel electrophoresis SH, Src homology synArfGEF(Po), potential synaptic Arf-GEF VGAT, vesicular γ-aminobutyric acid transporter XLID, non-syndromic X chromosome-linked intellectual disability Acknowledgements We thank Dr. Kazuhisa Nakayama (Kyoto University Graduate School of Pharmaceutical Sciences) for the expression vectors for HA-tagged Arf1, Arf6 and GST-GGA1-GAT fusion protein, Dr. Masanobu Satake (Tohoku University) for FLAG-tagged Arf6 in pcDNA3, Dr. James M. Ervasti (University of Minnesota) for FLAG-utrophin in pFASTBac1, Dr. J. Miyazaki (Osaka University Medical School) for pCAGGS vector. This work was supported by Grants-in-Aid for Scientific Research to H.S. (#2165049 and #22300114) from the Ministry of Education, Science, Sports, Culture, and Technology of Japan and grants from Kitasato University (All Kitasato Project Study and Integrative Research Program #2009-A01).
References Brunig, I., Suter, A., Knuesel, I., Luscher, B. and Fritschy, J. M. (2002) GABAergic terminals are required for postsynaptic clustering of dystrophin but not of GABAA receptors and gephyrin. J Neurosci, 22, 4805-4813. Charrier, C., Ehrensperger, M. V., Dahan, M., Levi, S. and Triller, A. (2006) Cytoskeleton regulation of glycine receptor number at synapses and diffusion in the plasma membrane. J Neurosci, 26, 8502-8511. Chen, H. I. and Sudol, M. (1995) The WW domain of Yes-associated protein binds a proline-rich ligand that differs from the consensus established for Src homology 3-binding modules. Proc Natl Acad Sci U S A, 92, 7819-7823. Chen, H. J., Rojas-Soto, M., Oguni, A. and Kennedy, M. B. (1998) A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II. Neuron, 20, 895-904. Choi, S., Ko, J., Lee, J. R., Lee, H. W., Kim, K., Chung, H. S., Kim, H. and Kim, E. (2006) ARF6 and EFA6A regulate the development and maintenance of dendritic spines. J Neurosci, 26, 4811- 4819. Claing, A. (2004) Regulation of G protein-coupled receptor endocytosis by ARF6 GTP-binding proteins. Biochem Cell Biol, 82, 610-617. Cox, R., Mason-Gamer, R. J., Jackson, C. L. and Segev, N. (2004) Phylogenetic analysis of Sec7- domain-containing Arf nucleotide exchangers. Mol Biol Cell, 15, 1487-1505. D'Souza-Schorey, C. and Chavrier, P. (2006) ARF proteins: roles in membrane traffic and beyond. Nat Rev Mol Cell Biol, 7, 347-358. D'Souza-Schorey, C., Li, G., Colombo, M. I. and Stahl, P. D. (1995) A regulatory role for ARF6 in receptor-mediated endocytosis. Science, 267, 1175-1178. Delaney, K. A., Murph, M. M., Brown, L. M. and Radhakrishna, H. (2002) Transfer of M2 muscarinic acetylcholine receptors to clathrin-derived early endosomes following clathrin- independent endocytosis. J Biol Chem, 277, 33439-33446. Dosemeci, A., Makusky, A. J., Jankowska-Stephens, E., Yang, X., Slotta, D. J. and Markey, S. P. (2007) Composition of the synaptic PSD-95 complex. Mol Cell Proteomics, 6, 1749-1760. Dunphy, J. L., Moravec, R., Ly, K., Lasell, T. K., Melancon, P. and Casanova, J. E. (2006) The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins. Curr Biol, 16, 315-320. Essrich, C., Lorez, M., Benson, J. A., Fritschy, J. M. and Luscher, B. (1998) Postsynaptic clustering of major GABAA receptor subtypes requires the γ2 subunit and gephyrin. Nat Neurosci, 1, 563-571. Feng, G., Tintrup, H., Kirsch, J., Nichol, M. C., Kuhse, J., Betz, H. and Sanes, J. R. (1998) Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science, 282, 1321-1324.
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SynArfGEF is a guanine nucleotide exchange ... - Pharmacy Eprints

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