Abbreviations Arf, ADP ribosylation factor AMPA, α-amino-3-hydroxy-5-methyl-4-<strong>is</strong>oxazolepropionate BRAG, brefeldin A-res<strong>is</strong>tant Arf-GEF DAPI, 4', 6-diamidino-2-phenylindole dihydrochloride DGC, dystrophin-associated glycoprotein complex DIV, day in vitro GABA, γ-aminobutyric acid GABAAR, GABAA receptor GAP, GTPase-activating protein GEF, <strong>guanine</strong> <strong>nucleotide</strong> <strong>exchange</strong> factor GGA1, Golgi-localizing, γ-adaptin ear homology domain, Arf-binding protein 1 GST, glutathione S- transferase HA, hemagglutinin IRSP, insulin receptor tyrosine kinase substrate of 53 kDa MAGI, membrane-associated guanylate kinase with inverted orientation MBP, maltose-binding protein Mena/VASP, mammalian enabled/vasodilator stimulated phosphoprotein NMDA, N-methyl-D- aspartate PB, phosphate buffer PBS, phosphate-buffered saline PDZ, PSD-95/D<strong>is</strong>cs large/Zona occludens 1 PSD, postsynaptic density PVDF, polyvinyl difluoride SDS, sodium dodecyl sulfate SDS-PAGE, SDS-polyacrylamide gel electrophores<strong>is</strong> SH, Src homology synArfGEF(Po), potential synaptic Arf-GEF VGAT, vesicular γ-aminobutyric acid transporter XLID, non-syndromic X chromosome-linked intellectual d<strong>is</strong>ability Acknowledgements We thank Dr. Kazuh<strong>is</strong>a Nakayama (Kyoto University Graduate School of Pharmaceutical Sciences) for the expression vectors for HA-tagged Arf1, Arf6 and GST-GGA1-GAT fusion protein, Dr. Masanobu Satake (Tohoku University) for FLAG-tagged Arf6 in pcDNA3, Dr. James M. Ervasti (University of Minnesota) for FLAG-utrophin in pFASTBac1, Dr. J. Miyazaki (Osaka University Medical School) for pCAGGS vector. Th<strong>is</strong> work was supported by Grants-in-Aid for Scientific Research to H.S. (#2165049 and #22300114) from the Min<strong>is</strong>try of Education, Science, Sports, Culture, and Technology of Japan and grants from Kitasato University (All Kitasato Project Study and Integrative Research Program #2009-A01).
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