25OH Vitamin D Total Assay - University of California, San Francisco
25OH Vitamin D Total Assay - University of California, San Francisco
25OH Vitamin D Total Assay - University of California, San Francisco
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UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CLINICAL LABORATORIES<br />
3. Before loading an integral onto the instrument, check reagent compartments for bubbles and<br />
liquid adherence to the covers; remove if present.<br />
4. Newly prepared Wash/System Liquid must sit for 6 hours to de-gas prior to use on the instrument.<br />
5. Wash/System Liquid tanks must be bleached monthly according to manufacturer’s<br />
recommendations and rinsed thoroughly to prevent build up <strong>of</strong> residue from Wash/System Liquid,<br />
which could affect background readings. See maintenance procedures.<br />
VII. CALIBRATION / CALIBRATION VERIFICATION<br />
1. The predefined Master Curve is adjusted to a new instrument-specific curve using the two<br />
calibrators supplied in the Reagent Integral via the bar codes on the reagent integral label.<br />
2. Renewed calibration is required:<br />
a. With each new lot <strong>of</strong> reagents (Reagent Intergral or Starter Reagents).<br />
b. Every 7 days.<br />
c. After each servicing <strong>of</strong> the LIAISON Analyzer.<br />
d. If quality controls are out <strong>of</strong> acceptable range.<br />
3. Refer to the LIAISON Daily Quick Start Procedure for detailed information on how to perform an<br />
assay calibration.<br />
4. Refer to Appedix II for detailed information on how to perform a Calibration Verification.<br />
VIII. QUALITY CONTROL<br />
LIAISON 25 OH VITAMIN D TOTAL CONTROL SET (with Bar Code Labels for Controls 1 and 2)<br />
1. Controls are received in liquid form and require no further dilution prior to use.<br />
2. The controls are stored at 2 – 8˚C upon receipt. When stored and handled as directed, the<br />
reagent is stable until the expiration date.<br />
3. LIAISON 25 OH VITAMIN D TOTAL controls levels 1 and 2 are run each day <strong>of</strong> testing.<br />
IX. PROCEDURE<br />
ASSAY PROCEDURE<br />
For detailed instructions on the following steps, refer to LIAISON Daily Quick Start Procedure.<br />
For additional information on principles <strong>of</strong> operation, refer to the LIAISON User Manual.<br />
1. Prepare the Analyzer.<br />
a. Prepare Reagent Intergals.<br />
b. Check Instrument Status.<br />
c. Check Status <strong>of</strong> Data from Prior Run.<br />
d. Check Printer Status.<br />
2. Perform System Test.<br />
3. Perform Calibration if required.<br />
4. Run LIAISON 25 OH VITAMIN D TOTAL controls levels 1 and 2.<br />
5. Program, place and run patient samples.<br />
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