25OH Vitamin D Total Assay - University of California, San Francisco

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REAGENTS UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CLINICAL LABORATORIES 2. LIAISON Module (Cat. No. 319130) 3. LIAISON Starter Kit (Cat. No. 319102) 4. LIAISON Light Check (Cat. No. 319101) 5. LIAISON Wash/System Fluid (Cat No. 319100) 6. LIAISON Waste Bags (Cat. No. 9450003) 7. LIAISON 25 OH Vitamin D TOTAL Control Set (Cat. No. 310601) 8. LIAISON 25 OH Vitamin D TOTAL Specimen Diluent (Cat. No. 310602) 9. LIAISON 25 OH Vitamin D TOTAL Calibration Verifiers (Cat. No. 310603) 10. LIAISON Cleaning Kit (Cat. No. 310990) 11. LIAISON Xcelerator (Cat. No. A0090) 12. Centrifuge (IEC Centra-7, International Equipment Company) 13. 12 x 75 glass or plastic tubes 14. Transfer pipettes 15. Calibrated pipettes for delivering volumes of 75 µL and 225 µL 16. For information on materials required for maintenance procedures, refer to the DiaSorin Liaison User Manual, Chapter 18. DIASORIN LIAISON REAGENT INTEGRAL, 100 TESTS, the following components are all contained within the Reagent Integral: 1. Magnetic Particles (2.4 mL) Magnetic particles coated with antibody against 25 OH Vitamin D, protein, phosphate buffer, < 0.1% sodium azide. 2. Assay Buffer (28.0 mL) Buffer with 10% ethanol, surfactants and preservatives. 3. Conjugate (4.5 mL) 25 OH Vitamin D conjugated to an isoluminol derivative, in phosphate buffer with 10% ethanol, EDTA, surfactant and preservatives. 4. Calibrator 1 (1.0 mL) Human serum, Tris Buffer, < 0.1% sodium azide and 25 OH Vitamin D. The calibrator concentrations (ng/mL) are referenced to standard preparations containing highly purified 25 OH Vitamin D. 5. Calibrator 2 (1.0 mL) Human serum, Tris Buffer, < 0.1% sodium azide and 25 OH Vitamin D. The calibrator concentrations (ng/mL) are referenced to standard preparations containing highly purified 25 OH Vitamin D. OTHER REAGENTS 1. LIAISON STARTERS eterr:Library:Mail Downloads:25OHVitDTotal.doc Page 4 of 15
UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CLINICAL LABORATORIES a. LIAISON STARTER 1: Catalyst in 4% NaOH b. LIAISON STARTER 2: 0.12% peroxide solution 2. LIAISON WASH/SYSTEM LIQUID: Phosphate buffer solution (10x concentrate). Preservative: sodium azide. 3. LIAISON LIGHT CHECK 4. LIAISON 25 OH VITAMIN D TOTAL CONTROL SET (with Bar Code Labels for Controls 1 and 2) a. CONTROL LEVEL 1: Human serum with buffer salts and < 0.1% sodium azide as a preservative. b. CONTROL LEVEL 2: Human serum with buffer salts and < 0.1% sodium azide as a preservative. 5. LIAISON 25 OH VITAMIN D TOTAL SPECIMEN DILUENT: Human serum with buffer salts and
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25OH Vitamin D Total Assay - University of California, San Francisco

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