25OH Vitamin D Total Assay - University of California, San Francisco

REAGENTS
UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CLINICAL LABORATORIES
2. LIAISON Module (Cat. No. 319130)
3. LIAISON Starter Kit (Cat. No. 319102)
4. LIAISON Light Check (Cat. No. 319101)
5. LIAISON Wash/System Fluid (Cat No. 319100)
6. LIAISON Waste Bags (Cat. No. 9450003)
7. LIAISON 25 OH Vitamin D TOTAL Control Set (Cat. No. 310601)
8. LIAISON 25 OH Vitamin D TOTAL Specimen Diluent (Cat. No. 310602)
9. LIAISON 25 OH Vitamin D TOTAL Calibration Verifiers (Cat. No. 310603)
10. LIAISON Cleaning Kit (Cat. No. 310990)
11. LIAISON Xcelerator (Cat. No. A0090)
12. Centrifuge (IEC Centra-7, International Equipment Company)
13. 12 x 75 glass or plastic tubes
14. Transfer pipettes
15. Calibrated pipettes for delivering volumes of 75 µL and 225 µL
16. For information on materials required for maintenance procedures, refer to the DiaSorin Liaison
User Manual, Chapter 18.
DIASORIN LIAISON REAGENT INTEGRAL, 100 TESTS, the following components are all contained
within the Reagent Integral:
1. Magnetic Particles (2.4 mL) Magnetic particles coated with antibody against 25 OH Vitamin D,
protein, phosphate buffer, < 0.1% sodium azide.
2. Assay Buffer (28.0 mL) Buffer with 10% ethanol, surfactants and preservatives.
3. Conjugate (4.5 mL) 25 OH Vitamin D conjugated to an isoluminol derivative, in phosphate buffer
with 10% ethanol, EDTA, surfactant and preservatives.
4. Calibrator 1 (1.0 mL) Human serum, Tris Buffer, < 0.1% sodium azide and 25 OH Vitamin D.
The calibrator concentrations (ng/mL) are referenced to standard preparations containing highly
purified 25 OH Vitamin D.
5. Calibrator 2 (1.0 mL) Human serum, Tris Buffer, < 0.1% sodium azide and 25 OH Vitamin D.
The calibrator concentrations (ng/mL) are referenced to standard preparations containing highly
purified 25 OH Vitamin D.
OTHER REAGENTS
1. LIAISON STARTERS
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