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UNIVERSITE DE BOURGOGNE THÈSE Yongbo LIU - Université de ...

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four groups, 50 % of plants were clipped. CP and NC plants were alternately planted within<br />

each pot.<br />

When th e f irst s iliques w ere m atured completely (i.e. yellow in c olor), th e s iliques<br />

were counted on every plant, then all the plants were cut at the base and left to dry at room<br />

temperature, an d t hey were t hreshed t o co llect t he s eeds. S tems an d l eaves an d s ilique<br />

material, e xcept s eeds, were dr ied i n a n ove n a t 80 °C f or 48 h, a nd f inal dr y w eight w as<br />

measured. Seed number per plant was calculated. The averaged CP and NC data per plot were<br />

used in fixed-effects ANOVA, and we used a 0.05 probability level for statistical significance.<br />

RESULTS<br />

Effects of different percentages of NC plants<br />

The ANOVA showed that clipping leaves had a significant effect on the three measures. NC<br />

plants pr oduced m ore s iliques, hi gher bi omass and m ore s eeds ( Table 3.1) t han C P. N o<br />

difference was obs erved a mong t reatments for t he N C pe rcentage, and t here w as no<br />

interaction (Table 3.1).<br />

Among the different NC percentages, no difference was recor<strong>de</strong>d for the three measures of<br />

NC: s ilique num ber ( df=3, F =1.32, P =0.30), bi omass ( F=1.20, P =0.35), a nd s eed num ber<br />

(F=0.68, P=0.58). For CP, different treatments showed different silique number (df=3, F=5.58,<br />

P=0.012) and biomass (F=7.88, P= 0.0036), but not different seed number (F=1.93, P= 0.18).<br />

CP plants in pure stands produced two times more than in 50% mixed stands.<br />

When l ooking a t t he given N C pe rcentage t reatments, s ome s ignificant di fferences<br />

appeared between NC and CP in spite of high variability. At the mixed stand of 75% NC, NC<br />

population pr oduced m ore s iliques ( df=1, F =8.11, P =0.029) a nd hi gher biomass ( F=21.97,<br />

P=0.003) than CP on average. At the 50% mixed stand, the differences reached a maximum:<br />

the average silique number (80±7) was 2.9-fold more than CP (27±4) (F=8.11, P=0.029); the<br />

biomass of NC (10.84±0.36) was 3.2-fold more than CP (3.29±0.19) (F=21.97, P=0.003); and<br />

seed number (460±43) of NC was 3.3-fold more than CP (138±16) (F=11.92, P=0.014). There<br />

was no difference at the 25 % NC treatment.<br />

85

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