UNIVERSITE DE BOURGOGNE THÈSE Yongbo LIU - Université de ...
UNIVERSITE DE BOURGOGNE THÈSE Yongbo LIU - Université de ...
UNIVERSITE DE BOURGOGNE THÈSE Yongbo LIU - Université de ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Characteristics of the backcrosses<br />
G1 peak values of flow cytometry results of BC1N plants were Normally distributed: N (m =<br />
101.3, σ = 8.7, p = 0.82) (Fig. 2.4). The G1 peak values of BC1J plants also fitted a Normal<br />
law distribution, N (m = 95.6, σ = 6.7, p= 0.36), but the mean value was significantly lower<br />
than that of BC1N by 5.7 units (t = 2.9, P =0.007), very close to that of the control B. juncea<br />
parent (m = 93.7).<br />
Both direct seedling treatment and phenotype i nference f rom pr ogeny analysis of t he<br />
plants g rown i n t he f ield e xperiment s howed e xpected M en<strong>de</strong>lian r atios: 3: 1<br />
resistant:susceptible in F 2, and 1:1 for both BC1J and BC1N p lants(Table 2 .5). Some plants<br />
growing in the field and <strong>de</strong>termined as susceptible produced at least one resistant <strong>de</strong>scendant<br />
(Table 2.6): 13 BC1NS i ndividuals, l ater on referenced BC1NS*, s howed 0.8 ± 0.6 %<br />
resistance on a verage i n t heir pr ogeny ( 0.34 % for a ll t he BC1NS pl ants); t he 4 F 2S pl ants<br />
produced 3.6 % resistant progeny; and 2 conventional herbici<strong>de</strong>-susceptible B. napus plants<br />
had 0.13 % resistant <strong>de</strong>scendants (0.007 % for all the B. napus plants).<br />
Table 2.5 Segregation of herbici<strong>de</strong>-resistant and susceptible plants in BC1N and F2, and Chisquare<br />
(χ 2 ) test against expected Men<strong>de</strong>lian segregation ratio (NS, not significant, p > 0.05)<br />
Plant type No. of plants Resistant Susceptible Expected ratio χ 2<br />
Data of direct seedling treatment<br />
F2 166 120 46 3:1 0.65 NS<br />
BC1N 50 27 23 1:1 0.32 NS<br />
BC1J 16 8 8 1:1 0 NS<br />
Data inferred from progeny analysis<br />
F2 25 21 4 3:1 1.08 NS<br />
BC1N 62 27 35 1:1 1.03 NS<br />
66