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UNIVERSITE DE BOURGOGNE THÈSE Yongbo LIU - Université de ...

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silique number, seed number, seed weight, biomass, number of viable seeds (all P < 0.001),<br />

and seed number per silique (P < 0.05) than NC plants. The clipping effect was less evi<strong>de</strong>nt in<br />

the s econd ex periment; i n t his cas e C P p lants h ad f ewer s iliques, l ighter s eed w eights, a nd<br />

lower biomass than NC plants (Figure 3.5, Table 3.5). Clipping had no effect on flowering<br />

date, number of viable seeds, or the resource allocation in<strong>de</strong>x in either year (Table 3.5).<br />

Effect of the percentage of simulated insect-resistant plants<br />

In the 2008 experiment, an increased percentage of NC plants resulted in reduced seed<br />

weight, biomass, and number of viable seeds in both plant types (P < 0.01 for all variables),<br />

though t he pr esence a nd m agnitu<strong>de</strong> o f t his e ffect varied w ith pl ot pos ition. T here was no<br />

effect o n f lowering t ime o r t he r esource al location i n<strong>de</strong>x. T here w as al so a s ignificant<br />

interaction between leaf clipping, the percentage of NC plants, and plot position (Table 3.5),<br />

suggesting that NC and CP plants reacted differently to different combinations of conditions.<br />

In t he p lot cen tre, an i ncreased p ercentage o f N C p lants resulted i n d ecreased s eed w eight,<br />

biomass, and number of viable seeds of both plant types. However, at the plot bor<strong>de</strong>r, these<br />

variables only <strong>de</strong>creased for NC plants (Figure 3.6, Table 3.6). Each of these variables was<br />

plotted against the percentage of NC plants separately for each plant type at the bor<strong>de</strong>r, and<br />

again f or t hose a t t he c enter of t he pl ots ( Figure 3 .6). T he r egression s lopes di d not di ffer<br />

between NC and CP plants situated at the center of the plot. The slopes for NC plants situated<br />

at the bor<strong>de</strong>r did not differ from those situated at the centre. In all cases, the low values of the<br />

regression coefficient R 2 indicated a poor fit and a great <strong>de</strong>al of variability among plots (Table<br />

3.6).<br />

In the 2009 e xperiment, there was no l ink between the percentage of NC plants and<br />

any of the measured characteristics, and no interaction among treatments. There was only one<br />

significant lin ear r egression; f lowering d ate o f th e N C p lants s ituated a t th e p lot c enter<br />

increased with the percentage of NC plants (Figure 3.6).<br />

In the 2008 e xperiment, the relationship between the variables and percentage of NC<br />

plants was non-linear for CP plants at the plot bor<strong>de</strong>r ( Figure 3.6). The resource allocation<br />

in<strong>de</strong>x was lowest at T 50 for all NC and CP plants at both plot positions (Figure 3.6). In the<br />

2009 experiment, the relationship between the variables and percentage of NC plants was also<br />

non-linear for both NC and CP plants at the plot center. In this experiment, extreme values of<br />

NC plants were observed at T75 in the plot bor<strong>de</strong>r and the resource allocation in<strong>de</strong>x of NC and<br />

106

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