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Diversifying crop rotations with temporary grasslands - Université de ...

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B.III.2.4 Cutting height<br />

In 2008, the effect of cutting height was tested for four species (containing <strong>crop</strong>s and weeds,<br />

grasses and broadleaved species). Seven treatments were compared varying by the cutting<br />

height and the quantity of leaves and buds remaining after cutting (Bonnot, 2008).<br />

B.III.2.5 Interactions between cutting and competition<br />

In 2008, interactions between cutting and competition were analyzed for plants of 12 weed<br />

species grown in small experimental plant communities. Cutting and competition treatments<br />

were crossed using a 2x2 factorial <strong>de</strong>sign where the potential interactions could be analyzed<br />

(Article 5).<br />

B.III.3 Cutting treatment and data collection<br />

In all experiments except the tests of cutting height, plants were manually cut at a standardized<br />

height (about 5 cm above soil surface) using a pair of scissors. As a control, 4 supplementary<br />

plants of each species were left uncut to compare their productivity <strong>with</strong> the cumulated<br />

productivity of cut plants (see <strong>de</strong>tails in Article 4). In some experiments, the cutting treatments<br />

were repeated several times, i.e. regrown shoots were repeatedly cut at the same height to<br />

analyze long-term effects. For each species and experimental modality, at least 4 replicate<br />

plants were used, in some experiments up to 12.<br />

Weed regrowth was characterized by measuring the aboveground biomass production until the<br />

next cutting. Moreover, non-<strong>de</strong>structive measurements of the plant height and the leaf area<br />

were used in some experiments to record regrowth dynamics between the cuttings. The leaf<br />

area was <strong>de</strong>termined by image analysis of plant photos in collaboration <strong>with</strong> two master<br />

stu<strong>de</strong>nts co-supervised by the present author, Fre<strong>de</strong>ric Henriot (2007), and Rémi Bonnot<br />

(2008), see also Article 4 for <strong>de</strong>tails.<br />

B.IV FIELD EXPERIMENTS ANALYZING WEED SEED PREDATION<br />

B.IV.1 Rationale<br />

Unlike most annual <strong>crop</strong>s, PFCs are characterized by the complete absence of soil tillage as<br />

well as permanent vegetation during several years. Both characteristics may have various<br />

44

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