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BIOBAR : USER MANUAL

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Only DNA sequences can be used.. An error message will be displayed if<br />

a protein or RNA sequence is selected. If both DNA and protein<br />

sequences are selected, an error message will be displayed and %GC will<br />

only be calculated for the DNA sequence(s).<br />

Calculates the %GC content for the entire sequence and displays it in a<br />

table.<br />

o Window %GC<br />

Only DNA sequences can be used.. An error message will be displayed if<br />

a protein or RNA sequence is selected. If both DNA and protein<br />

sequences are selected, an error message will be displayed and %GC will<br />

only be calculated for the DNA sequence(s).<br />

Calculates the %GC content for windows of length defined by user in the<br />

Sequence Statistics Options and displays them in a table, indexed by<br />

position.<br />

o Nucleotide Frequencies<br />

Only DNA sequences can be used. An error message will be displayed if a<br />

protein or RNA sequence is selected. If both DNA and protein sequences<br />

are selected, an error message will be displayed and nucleotide<br />

frequencies will only be calculated for the DNA sequence(s).<br />

Calculates frequency of each nucleotide for the entire sequence and<br />

displays it in a table.<br />

o N-Gram<br />

Only DNA sequences can be used. An error message will be displayed if<br />

a protein or RNA sequence is selected. If sequences of all types are<br />

selected, the N-Grams will only be calculated for the DNA sequence(s).<br />

IUB characters will be ignored<br />

Takes the value of N from Sequence Statistics Options and generates a list<br />

of all possible “words” of length N (from the characters A, T, C, G) and<br />

their counts within the sequence.<br />

See Sequence Statistics Options for other options relating to N-Grams<br />

o Codon Usage Table<br />

Only DNA sequences can be used. An error message will be displayed if<br />

a protein or RNA is selected. If sequences of all types are selected, the<br />

codon usage table will only be generated for the DNA sequence(s). IUB<br />

characters will be ignored<br />

Generates a codon usage table (using the offset from Sequence Statistics<br />

Options) using the sequence(s) that are selected.<br />

See Sequence Statistics Options for options relating to table display<br />

o Calculate Molecular Weight<br />

Open to DNA, RNA and protein sequences.

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