Lab Safety Manual - UCLA - Environment, Health & Safety

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of the nine readings must be at least 100 linear feet per minute (lfm) with a minimum of 70 lfm for any measurement. The average face velocity should not exceed 160 lfm. Each fume hood must be equipped with at least one type of continuous quantitative monitoring device designed to provide the user with current information on the operational status of the hood. Many hoods also have motion sensors to determine when they are not in active use. These sensors will reduce the fume hood‟s air flow as part of the campus‟ energy savings effort. When hazardous materials are in a fume hood, but it is not under active use (e.g., during an unattended reaction or experiment), the sash should be closed. Fume hoods are not designed for storage of hazardous materials. Routine maintenance and repairs of fume hoods are conducted by Facilities Management. Hood users may route requests for hood repair directly to Facilities via Facilities Service Requests (FSR). Make sure to indicate that the FSR has been “generated as a result of a health and safety deficiency” and mark it “urgent” in order to expedite processing. EH&S does not initiate maintenance but will coordinate with Facilities Management to ensure that it is completed. Upon reported completion by Facilities, EH&S will re-inspect the fume hood following maintenance or repairs. General Rules for Fume Hood Use The following general rules should be followed when using laboratory hoods: 1. Fume hoods should not be used for work involving hazardous substances unless they have a certification label that confirms certification has occurred within the past year 2. Always keep hazardous chemicals >6 inches behind the plane of the sash 3. Never put your head inside an operating laboratory hood. The plane of the sash is the barrier between contaminated and uncontaminated air 4. Work with the hood sash in the lowest practical position. The sash acts as a physical barrier in the event of an accident. Keep the sash closed when not conducting work in the hood 5. Do not clutter your hood with unnecessary bottles or equipment. Keep it clean and clear. Only materials actively in use should be in the hood 6. Do not make any modifications to hoods, duct work, or the exhaust system without first contacting the EH&S office via the EH&S Hotline at 310-825-9797 7. Do not use large equipment in laboratory hoods unless the hood is dedicated for this purpose, as large obstructions can change the airflow patterns and render the hood unsafe 8. Shut your sash! For energy efficiency, make sure to shut your sash when the hood is not in use Laboratory fume hoods are one of the most important pieces of equipment used to protect laboratory and other workers from exposure to hazardous chemicals. Chemical fume hoods should be inspected upon installation, renovation, when a deficiency is reported, or a change has been made to the operating characteristics of the hood. Since fume hoods used for regulated carcinogens (as listed in the UCLA Particularly Hazardous Substances Policy: http://www.adminpolicies.ucla.edu/app/Default.aspx?&id=907) have additional requirements, such as increased face velocity (average of 100-150 lfm, with no measurement less than 100 lfm), contact the EH&S Hotline at 310-825-9797 if the intended use changes. Chemical Hygiene Plan 4-3 January 2013
Fume Hood Inspections Step 1 – Physical Inspection Step 2 – Hood Performance Inspection Evaluates the physical condition of the hood and the materials being used in the hood. This includes checking for: Improper storage of materials inside the fume hood Use of proper materials General hood cleanliness Physical damage to the fume hood (e.g., broken sash) Fully functioning lighting, fume hood indicator, airflow monitor, and alarm Glove Boxes and Ventilation Devices Evaluates the overall hood performance to ensure that it is functioning properly. This involves checking the: Average face velocity and set minimum face velocity, which is used to determine the rating of the hood and what the hood can be used for Noise generated by the fume hood, to ensure that it is below 85 dB If fume hood does not pass inspection, it will be labeled with a “DO NOT USE” sign until it can be repaired. Labs should complete a Facilities Service Consent Form (Appendix S) In addition to fume hoods, some laboratories use contained glove box units for working with reactive chemicals under an inert environment, working with very toxic substances in a completely closed system, or for creating a stable, breeze free, system for weighing hazardous or reactive materials. These units can be very effective because they offer complete containment. Other Engineering Controls In addition to the elements listed above, consideration must be given to providing sufficient engineering controls for the storage and handling of hazardous materials. No more than 10 gallons of flammable chemicals may be stored outside of an approved flammable storage cabinet. For refrigerated or frozen storage, flammable and explosive materials must be kept in refrigeration units specifically designed for storing these materials. Generally these units do not have internal lights or electronic systems that could spark and trigger an ignition; additionally, the cooling elements are external to the unit. These units should be labeled with a rating from Underwriters Laboratory or other certifying organization Secondary containment must be provided for corrosive and reactive chemicals and is recommended for all other hazardous chemicals. Secondary containment should be made of chemically resistant materials and should be sufficient to hold the volume of at least the largest single bottle stored in the container. Laboratories that use hazardous materials must contain a sink, kept clear for hand washing to remove any final residual contamination. Hand washing is recommended whenever a staff member who has been working with hazardous materials plans to exit the laboratory or work on a project that does not involve hazardous materials. ADMINISTRATIVE CONTROLS The next layer of safety controls are Administrative Controls. These controls consist of policies and procedures; they are not generally as reliable as engineering controls in that the user has to carefully follow the appropriate procedures and must be fully trained and aware in order to do so. Chemical Hygiene Plan 4-4 January 2013
Page 2 and 3: Laboratory Safety Manual Office of
Page 4 and 5: esponse. This information is intend
Page 6 and 7: are noted, and provide training and
Page 8 and 9: IIPP
Page 10 and 11: Chemical Hygiene Plan January 2013
Page 12 and 13: Table of Contents Chapter 1: Introd
Page 14 and 15: Chapter 1: Introduction Purpose UCL
Page 16 and 17: All personnel, including PIs/Labora
Page 18 and 19: c. Preparing SOPs and performing li
Page 20 and 21: Chapter 2: Chemical Hazard Communic
Page 22 and 23: EMPLOYEE INFORMATION AND TRAINING E
Page 24 and 25: Chapter 3: Classes of Hazardous Che
Page 26 and 27: esistant storage cabinet or, in man
Page 28 and 29: Acute Toxins Substances that have a
Page 30 and 31: nanomaterials are used as fine powd
Page 32 and 33: Chapter 4: How to Reduce Exposures
Page 36 and 37: EH&S requires that each laboratory
Page 38 and 39: EH&S policy requires each laborator
Page 40 and 41: An annual refresher is required for
Page 42 and 43: Never use mouth suction for pipetti
Page 44 and 45: Chapter 5: Chemical Exposure Assess
Page 46 and 47: the proper respiratory equipment an
Page 48 and 49: Physician’s Written Opinion For e
Page 50 and 51: Chapter 6: Inventory, Labeling, Sto
Page 52 and 53: Chemical Storage & Segregation Esta
Page 54 and 55: flammable solvent. Other common haz
Page 56 and 57: fuel-gas cylinders securely in the
Page 58 and 59: Chapter 7: Training Regulatory Requ
Page 60 and 61: Resources EH&S has a number of tool
Page 62 and 63: Chapter 8: Inspections and Complian
Page 64 and 65: and safety training, including safe
Page 66 and 67: Chapter 9: Hazardous Chemical Waste
Page 68 and 69: The manufacturer‟s SDS provides d
Page 70 and 71: Peroxide Forming Chemicals Peroxide
Page 72 and 73: appropriate lab coat, gloves and ey
Page 74 and 75: BENCH TOP TREATMENT EPA regulations
Page 76 and 77: Chapter 10: Accidents and Chemical
Page 78 and 79: Chemical Spills Chemical spills can
Page 80 and 81: Appendices Chemical Hygiene Plan Ja
Page 82 and 83: Appendix A: General Rules for Labor
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UNSAFE LABORATORY HABITS Personal P
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Appendix B: Sample Safety Data Shee
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Sample SDS, cont. Obtain SDS from t
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Appendix C: Container Labeling Chem
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Appendix D: SOP Instructions and Te
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Sample SOP Template Obtain this for
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Sample SOP Template, cont. Obtain t
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Sample SOP Template, cont. Obtain t
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Appendix E: UCLA Particularly Hazar
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substances or generalized over a gr
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E. Engineering Controls 1. Bench to
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educe exposure. If levels cannot be
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4. It is listed in either Group 2A
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A T T A C H M E N T C Listed Carcin
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Appendix F: Regulated Carcinogens C
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Appendix G: Respiratory Hazard Asse
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Appendix H: Peroxide Forming Chemic
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Appendix I: EH&S Safety Training Ma
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Appendix J: Employee Training Histo
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Appendix K: Site-Specific Training
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Appendix L: Laboratory Inspection C
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Laboratory Inspection Checklist, co
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Appendix M: Segregation of Incompat
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Appendix N: Hazardous Waste Pick-Up
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Appendix O: Spill Clean-up Procedur
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Appendix P: Lab Emergency Poster Ob
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Appendix Q: Policy 905 - Research L
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B. Protective gloves must be worn w
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Appendix R: NanoTool Kit Chemical H
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Chemical Hygiene Plan R-3 January 2
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Chemical Hygiene Plan R-11 January
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Appendix S: Facilities Service Cons
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Appendix T: PPE Issuance Form Chemi
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Appendix U: Glossary ACGIH - The Am
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GENERAL VENTILATION - Also known as
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MUTAGEN - Anything that can cause a
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Lab Specific SOPs
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Inspection Records
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Resources
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3. Outline Lab Safety Training Requ
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