Comparison of RAPD and PCR-RFLP markers for classification and ...
Comparison of RAPD and PCR-RFLP markers for classification and ...
Comparison of RAPD and PCR-RFLP markers for classification and ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
190<br />
Fatma H. Galal<br />
M 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3<br />
A B C D E<br />
Fig. 2: Representative 2% agarose gels <strong>of</strong> <strong>PCR</strong>-<strong>RFLP</strong> patterns generated from three<br />
ant species, C. maculatus, M. pharoensis <strong>and</strong> C. bicolor using <strong>for</strong>ty arbitrary<br />
primers. Lane M: DNA marker 100 bp Ladder, lane 1: C. maculatus, lane 2: C.<br />
bicolor <strong>and</strong> lane 3: M. pharoensis. A: <strong>PCR</strong> <strong>of</strong> ITS region, B: EcoRI, C: SacI,<br />
D: TaqI <strong>and</strong> E: MspI.<br />
Table 1: Average percentages <strong>of</strong> common <strong>and</strong> polymorphic b<strong>and</strong>s within each species as revealed by<br />
<strong>RAPD</strong> <strong>and</strong> <strong>RFLP</strong> analyses.<br />
Ant species Total no. <strong>of</strong><br />
<strong>RAPD</strong> molecular marker <strong>RFLP</strong> molecular marker<br />
tested b<strong>and</strong>s % Monomorphic % Polymorphic % Monomorphic % Polymorphic<br />
C. maculatus 556 200/256<br />
56/256<br />
164/300<br />
136/300<br />
78.1<br />
21.9<br />
54.7<br />
45.3<br />
M. pharoensis 741 216/396<br />
180/396<br />
250/345<br />
95/345<br />
54.5<br />
45.5<br />
72.5<br />
27.5<br />
C. bicolor 563 187/248<br />
61/248<br />
172/315<br />
143/315<br />
75.4<br />
24.6<br />
54.6<br />
45.4<br />
On the other h<strong>and</strong>, <strong>RFLP</strong> assay clarified that the highest average percentage <strong>of</strong><br />
monomorphic b<strong>and</strong>s (72.5%) was observed within M. pharoensis. Meanwhile, the<br />
highest average percentage <strong>of</strong> polymorphic b<strong>and</strong>s (45.4%) was observed within C.<br />
bicolor (Table1). Furthermore, <strong>RAPD</strong> analysis revealed that the highest average<br />
percentage <strong>of</strong> monomorphic b<strong>and</strong>s (90.7%) was observed between M. pharoensis <strong>and</strong><br />
C. bicolor. Meanwhile, the highest average percentage <strong>of</strong> monomorphic b<strong>and</strong>s<br />
resolved by <strong>RFLP</strong> (94.6%) was observed between C. maculatus <strong>and</strong> C. bicolor (Table<br />
2). On the other h<strong>and</strong>, the highest average percentages <strong>of</strong> polymorphic b<strong>and</strong>s resolved<br />
by <strong>RAPD</strong> <strong>and</strong> <strong>RFLP</strong> (18.4 <strong>and</strong> 15.8%) were observed between M. pharoensis <strong>and</strong> C.<br />
maculatus (Table 2).<br />
Table (2): Average percentages <strong>of</strong> common <strong>and</strong> polymorphic b<strong>and</strong>s between species as revealed by<br />
<strong>RAPD</strong> <strong>and</strong> <strong>RFLP</strong> analyses.<br />
Ant species Total no. <strong>of</strong> tested<br />
b<strong>and</strong>s<br />
<strong>RAPD</strong> molecular marker <strong>RFLP</strong> molecular marker<br />
M. pharoensis <strong>and</strong> C. maculatus 1297<br />
%<br />
Monomorphic<br />
532/652<br />
81.6<br />
M. pharoensis <strong>and</strong> C. bicolor 1304 584/644<br />
90.7<br />
C. maculatus <strong>and</strong> C. bicolor 1119 447/504<br />
88.7<br />
M. pharoensis, C. maculatus <strong>and</strong> C. bicolor 1860 603/900<br />
67.0<br />
%<br />
Polymorphic<br />
120/652<br />
18.4<br />
60/644<br />
9.3<br />
57/504<br />
11.3<br />
297/900<br />
33.0<br />
%<br />
Monomorphic<br />
543/645<br />
84.2<br />
562/660<br />
85.2<br />
582/615<br />
94.6<br />
586/960<br />
61.0<br />
%<br />
Polymorphic<br />
102/645<br />
15.8<br />
98/660<br />
14.8<br />
33/615<br />
5.4<br />
374/960<br />
39.0
Change language