2 - BACTERIOWEB
2 - BACTERIOWEB 2 - BACTERIOWEB
How to characterize efflux mechanisms Plasmid or transposon encoded efflux systems – Multiresistance phenotype – Detection of efflux gene(s): PCR, nucleic probes Upregulation of intrinsic efflux systems – Protein levels ♦ Western blotting of membrane extracts with specific antibodies – mRNA levels ♦ Northern blot, MacroArray, MicroArray ♦ Real Time RT-PCR (Light Cycler, Taq Man, I Cycler…) – Intracellular accumulation of antibiotics ♦ [ 3 H] ou [ 14 C] radiolabeled or fluorescent compounds (BET, acriflavine…) – Sequencing of regulatory genes 40
Efflux inhibitors Phenyl-Arginyl ß N-naphtylamide 41
- Page 1 and 2: Role of the Efflux Pumps in Antimic
- Page 3 and 4: Cell wall Bacterial targets for ant
- Page 5 and 6: Drug inactivation Target alteration
- Page 7 and 8: Intracellular accumulation Drug acc
- Page 9 and 10: 9 Other bacterial species with know
- Page 11 and 12: Structure of bacterial efflux syste
- Page 13 and 14: PMF secondary transporters Major F
- Page 15 and 16: Efflux-based resistance in Staphylo
- Page 17 and 18: System NorA in S. aureus Antibiotic
- Page 19 and 20: Combination of mechanisms in S. aur
- Page 21 and 22: PmrA-mediated resistance in S. pneu
- Page 23 and 24: Other Gram positives Species System
- Page 25 and 26: 25 Efflux pumps coded by mobile gen
- Page 27 and 28: Efflux pumps of the RND family Bact
- Page 29 and 30: System AcrAB-TolC in E. coli Antibi
- Page 31 and 32: Induction of acrAB-tolC expression
- Page 33 and 34: RND efflux systems in P. aeruginosa
- Page 35 and 36: Acquired resistance in P. aeruginos
- Page 37 and 38: MexXY-mediated adaptive resistance
- Page 39: Therapeutic implications of efflux
Efflux inhibitors<br />
Phenyl-Arginyl ß N-naphtylamide<br />
41
Change language