Insert - EnviroLogix

Highlights:
Quantitative and traceable
results in QuickScan
Read strips wet – no drying
necessary
Simple protocol
No incubation equipment
needed
Contents of Kit:
50 QuickTox Strips packed in
a moisture-resistant canister
50 reaction vials
100 pipette tips
Grain Buffer Concentrate
DB3 Buffer
Available in 50-strip
individual kit format or
bulk packaging
Items Not Provided:
Orbital/rotary shaker
Plastic sample cups with
lids* or other sample
extraction vessels
Water for diluting Grain
Buffer Concentrate
Graduated cylinder*
Pipette(s) to deliver 50 and
200 µL*
Timer
Scissors
QuickScan System*
Mini-centrifuge and vials*
*Available as accessories –
see list on Page 3
Measure Grain Buffer, add to
ground sample
Rev. 01-31-13
Catalog Number AQ 113 BG
Intended Use
QuickTox Kit
for
Ochratoxin-A
This Kit is designed to quickly extract and screen wheat for the presence of
Ochratoxin-A residues. The QuickTox Kit is designed to provide quantitative results
in wheat grain for Ochratoxin-A residues ranging from 1.5 ppb to 30 ppb in the
standard assay, and up to 100 ppb with additional dilution. The kit has been certified
as a Performance Tested Method SM , #021301 by the AOAC Research Institute for use
in wheat.
How the Test Works
A composite wheat sample is collected, ground and then extracted to solubilize any
Ochratoxin-A present. Each sample should be ground and extracted with room
temperature Grain Buffer. This extract is further diluted with DB3 Buffer for testing
with the QuickTox Kit.
Each QuickTox Strip has an absorbent pad at each end. The protective tape with the
arrow indicates which end of the strip to insert into the reaction vial. The sample
extract travels up the membrane strip and is absorbed into the larger pad at the top of
the strip. At ten minutes, the strip is cut off at the top of the arrow tape, the bottom
pads are discarded, and the strip is inserted into the QuickScan reader to obtain
quantitative results.
Preparation of the Sample
Please note: sample extract should be tested immediately after dilution with DB3
Buffer (Steps 6&7). Make sure strips and DB3 Buffer are at room temperature and
ready for use before the dilution step.
Determine size of sample
1. Collect a composite wheat sample according to your own sampling plan or
USDA/GIPSA guidelines. Consult USDA/GIPSA reference documents such as
http://archive.gipsa.usda.gov/reference-library/handbooks/grain-insp/
grbook1/bk1.pdf to help design a plan that fits your needs.
2. Grind samples using a grinder or mill which provides a sample with a
consistency comparable to the standard setting of "Turkish" on a Bunn grinder.
Mix ground material thoroughly before sub-sampling. The ground sample
should pass through a 20-mesh sieve.
Prepare Grain Buffer
3. In a clean vessel with a cover, dilute the Grain Buffer Concentrate 20 fold using
19 parts water and one part concentrate. Mix to make homogenous. (For
example: 25 mL Buffer Concentrate into 475 mL water.). Tap water may be
used for dilution, but purified water (distilled and/or deionized) yields optimal
results. If prepared with purified water, Grain Buffer can be stored for up to 7
days at room temperature. If prepared with tap water, Buffer should be used the
same day, or may be stored refrigerated for longer life (note: bring Buffer to
room temperature before testing).

Shake mechanically or by hand
Remove a portion of extract to
centrifuge tube and spin
for 3 minutes at 2000 x g
Get a new tip, add Buffer to vial,
discard tip. Get another new tip,
add extract, mix well, discard tip.
Place strip in vial
Wait 10 minutes for results
Rev. 01-31-13
Extract ground wheat with Grain Buffer
QuickTox Kit for QuickScan Ochratoxin-A
Page 2 of 5
4. Weigh 20 to 50 grams of milled sample into a disposable sample cup with lid or
other suitable container and add five volumes of room temperature Grain Buffer
(5 mL per gram of sample, i.e. 20 grams, add 100 mL).
5. Cap sample cup tightly and place on shaker at the highest speed, or shake
vigorously by hand, for 30 seconds. Samples that are not thoroughly mixed may
adversely affect test results due to incomplete extraction.
6. Immediately remove a portion of the sample and centrifuge it for 3 minutes at
2000 x g (not RPM). Consult centrifuge manual for g force calculation, and
follow manufacturer’s instructions for operation and balancing.
Dilute sample with DB3 Buffer (use 2 separate pipette tips)
7. With a new pipette tip, transfer 50 µL of DB3 Buffer into the reaction vial.
Discard tip.
8. Using another new pipette tip, remove 200 µL from the centrifuged sample and
add to the reaction vial containing the DB3 Buffer and mix well with pipette by
stirring or drawing liquids up and down in the pipette tip. Discard pipette tip.
NOTE: Samples that are not thoroughly mixed and/or accurately pipetted will
adversely affect test results. After adding the sample, the final volume in the reaction
vial should be 250 µL. Do not reuse diluted samples. Always use a new reaction vial
and two pipette tips for each sample, and discard vials and tips after use.
For testing samples at levels greater than 30 ppb:
If after running and reading the test, the initial result is greater than 30 ppb ("> 30
ppb" on QuickScan), and further knowledge about the level of contamination is
desired, samples can be retested by further dilution of the sample extract.
1. In a separate tube (not provided), combine 1 mL of Grain Buffer and 200 µL of
the centrifuged sample. Mix well.
2. Using a calibrated pipette with a new tip, place 50 µL DB3 Buffer into a reaction
vial.
3. With a fresh pipette tip, add 200 µL of the newly diluted extract to the reaction
vial containing Buffer. Mix thoroughly.
4. Follow the instructions under How to Run. Select 1:6 under the dilution tab on
QuickScan Results Screen—the System will calculate and record the Ochratoxin-
A level in diluted samples.
How to Run the QuickTox Strip Test
1. Allow refrigerated canisters to come to room temperature before opening.
Remove the QuickTox Strips to be used. Avoid bending the strips. Reseal the
canister immediately.
2. Place the strip into the reaction vial containing the DB3 Buffer and sample
extract. The arrow tape on the end of the strip should point into the reaction vial.
3. Sample extract will travel up the strip (flow may not be visible immediately—
this is expected and normal). Reaction vials will stand on their own.
4. Allow the strip to develop for 10 minutes. Immediately cut off and discard the
bottom section of the strip covered by the arrow tape. Insert strip into the
QuickScan reader for quantitation.

Rev. 01-31-13
The intensity
of the
Control Line
and Test Line
will vary
depending on
Ochratoxin-A
levels
Control Line
Test Line
cut here
Cut strip and place in
QuickScan reader immediately —
no drying step!
Place strip in
QuickScan carrier
QuickTox Kit for QuickScan Ochratoxin-A
Page 3 of 5
Use of the QuickScan System
Detailed instructions for use of the QuickScan system are supplied with each unit, and
can also be found at www.envirologix.com/quickscan. In summary, a strip is inserted
face down in the carrier with the barcoded end closest to the handle. The carrier is
inserted into the reader and the strips are read by touching or clicking on the "Read
Test" area of the screen. Results are then recorded in the DataLog, allowing each
user to report and track data easily.
Results are reported in the range of 1.5 to 30 ppb. Results less than 1.5 ppb are
reported as " 30 ppb." If quantification is desired above 30 ppb, a further dilution of
the sample extract can be performed (see "For testing samples at levels greater than
30 ppb" above).
Kit Storage
This QuickTox Kit should be stored refrigerated. Note the shelf life on the kit box.
Prolonged exposure to high temperatures may adversely affect the test results. Do not
open the desiccated canister until ready to use the strips.
Cross-reactivity
The following mycotoxins have been tested with this kit and no false positive results
occurred at the 100 ppm level:
Aflatoxin B1
Fumonisin B1
Precautions and Notes
Vomitoxin (deoxynivalenol)
Zearalenone
Ochratoxin may be toxic and carcinogenic for humans. Avoid contact with skin
and mucous membranes. Gloves, protective eyewear, and protective apparel
should be worn. If exposure occurs, flush affected area with water. Liquids
containing ochratoxins should be treated by the addition of bleach (add a
minimum of 10% of the total volume for 10 minutes before disposal).
This product is currently not applicable for use in testing any other grains.
This assay is calibrated against reference samples supplied by Trilogy Analytical
Laboratory, Washington, MO, and other vendors and associated HPLC data.
As with all tests, it is recommended that results be confirmed by an alternative
method when necessary.
The assay has been optimized for use with the protocol provided in the kit.
Deviation from this protocol may invalidate the results of the test. Proper and
thorough mixing, along with accurate pipetting, are essential to accurate results.
QuickScan has the capability of reading up to 180 ppb. The assay has been
validated for samples containing up to 100 ppb Ochratoxin-A.
The results generated through the proper use of this diagnostic tool reflect the
condition of the working sample directly tested. Extrapolation as to the condition
of the originating lot, from which the working sample was derived, should be
based on sound sampling procedures and statistical calculations which address
random sampling effects, non-random seed lot sampling effects and assay system
uncertainty. A negative result obtained when properly testing the working

Rev. 01-31-13
QuickTox Kit for QuickScan Ochratoxin-A
Page 4 of 5
sample does not necessarily mean the originating lot is entirely negative for the
analyte or protein in question.
Strips must be read wet promptly at ten minutes.
Protect all components from hot or cold extremes of temperature when not in
use. Do not leave in direct sunlight or in vehicle.
For convenience, accessories can be ordered from EnviroLogix (see list, below).
Accessories
Available through EnviroLogix: Catalog #
QuickScan System ACC 131
Sample cups with lids ACC 012-50
(50/package) for samples up to 30 g; larger samples require different mixing vessels
Graduated cylinder ACC 023 (100 mL)
MiniPet pipette 50 µL ACC 051
MiniPet pipette 200 µL ACC 067
Microcentrifuge ACC 064E
Centrifuge Set ACC 010
(50/set) contains disposable pipettes and vials

For Technical Support
Contact Us At:
EnviroLogix
500 Riverside Industrial
Parkway
Portland, ME 04103-1486
USA
Tel: (207) 797-0300
Toll Free: 866-408-4597
Fax: (207) 797-7533
e-mail:
info@envirologix.com
website:
www.envirologix.com
Rev. 01-31-13
QuickTox Kit for QuickScan Ochratoxin-A
Page 5 of 5
LIMITED WARRANTY
EnviroLogix Inc. ("EnviroLogix") warrants the products sold hereunder ("the Products")
against defects in materials and workmanship when used in accordance with the applicable
instructions for a period not to extend beyond a product’s printed expiration date. If the
Products do not conform to this Limited Warranty and the customer notifies EnviroLogix in
writing of such defects during the warranty period, including an offer by the customer to return
the Products to EnviroLogix for evaluation, EnviroLogix will repair or replace, at its option,
any product or part thereof that proves defective in materials or workmanship within the
warranty period.
ENVIROLOGIX MAKES NO OTHER WARRANTIES, EXPRESS OR IMPLIED,
INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF
MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. The warranty
provided herein and the data, specifications and descriptions of EnviroLogix products
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out of accident or improper or abnormal use of the Products; against defects in products or
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received (if any) from the maker thereof of such non-EnviroLogix made products or
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whether based on this Limited Warranty or otherwise, shall EnviroLogix be liable for
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This Limited Warranty states the entire obligation of EnviroLogix with respect to the Products.
If any part of this Limited Warranty is determined to be void or illegal, the remainder shall
remain in full force and effect.
License
EnviroLogix has developed this kit using proprietary reagents.
EnviroLogix, the EnviroLogix logo, QuickTox, and QuickScan are trademarks of
EnviroLogix Inc.
© EnviroLogix 2013