physicochemical and functional properties of crawfish chitosan as ...
physicochemical and functional properties of crawfish chitosan as ...
physicochemical and functional properties of crawfish chitosan as ...
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w<strong>as</strong> reduced from four to three steps (excluding decolorization). No et al. (2002) studied the<br />
effects <strong>of</strong> elimination <strong>of</strong> deproteinization (DP) step or reduction <strong>of</strong> alkali treatment time on the<br />
<strong>physicochemical</strong> <strong>and</strong> <strong>functional</strong> <strong>properties</strong> <strong>of</strong> <strong>chitosan</strong> products; they reported that <strong>chitosan</strong><br />
prepared without DP h<strong>as</strong> comparable N2 content, <strong>and</strong> lower degree <strong>of</strong> acetylation, solubility, <strong>and</strong><br />
water <strong>and</strong> fat binding capacity. However, the end product is higher in molecular weight <strong>and</strong><br />
viscosity, <strong>and</strong> lower in dye binding capacity than traditional <strong>chitosan</strong> prepared with the DP<br />
treatment.<br />
However, a comprehensive study to examine the effects <strong>of</strong> process<br />
alteration/modification <strong>of</strong> <strong>chitosan</strong> production on various <strong>physicochemical</strong> characteristics <strong>and</strong><br />
<strong>functional</strong> <strong>properties</strong> <strong>of</strong> <strong>crawfish</strong> <strong>chitosan</strong> products h<strong>as</strong> not yet been reported. Hence, the aim <strong>of</strong><br />
this study w<strong>as</strong> to evaluate <strong>physicochemical</strong> <strong>and</strong> <strong>functional</strong> <strong>properties</strong> <strong>of</strong> <strong>crawfish</strong> <strong>chitosan</strong> <strong>as</strong><br />
affected by modification <strong>of</strong> process protocols from the traditional four b<strong>as</strong>ic processing steps<br />
(DP, DM, DC, <strong>and</strong> DA) used in the isolation <strong>of</strong> <strong>chitosan</strong> from <strong>crawfish</strong> shell w<strong>as</strong>te. The specific<br />
objectives, therefore, were to:<br />
1. Develop an optimum <strong>chitosan</strong> production process for our particular intended application;<br />
2. Study the <strong>physicochemical</strong> <strong>and</strong> <strong>functional</strong> <strong>properties</strong> <strong>of</strong> <strong>crawfish</strong> <strong>chitosan</strong>, prepared from<br />
modified process protocols, <strong>and</strong> compare these <strong>properties</strong> with those <strong>of</strong> commercial crab<br />
<strong>chitosan</strong>s;<br />
3. Evaluate how decoloration (DC) affects <strong>physicochemical</strong> <strong>and</strong> <strong>functional</strong> <strong>properties</strong> <strong>of</strong><br />
<strong>crawfish</strong> <strong>chitosan</strong>s;<br />
4. Determine the effects <strong>of</strong> reversing the steps such <strong>as</strong> demineralization (DM) <strong>and</strong><br />
deproteinization (DP) during the production <strong>of</strong> <strong>chitosan</strong> from <strong>crawfish</strong> shell on their<br />
<strong>physicochemical</strong> <strong>and</strong> <strong>functional</strong> <strong>properties</strong>;<br />
4