Consultant's Report - Minnesota State Legislature

Consultant's Report - Minnesota State Legislature Consultant's Report - Minnesota State Legislature

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Table 2. Summary ofLCso values. Expt Chemical Pre-fert LCso Post-fert LCso 24-h LCso 48-h LCso 72-h LCso 1 Cutrine 0.001 0.013 (0.006 0.019) 0.008 (0.002 - 0.014) 0.009 0.012 CUS04 - - 0.008 - 0.007 2 3' Cutrine - - - - - CUS04 0.004 (0.002 - 0.005) 0.005 - - - Cutrine 0.002 - - - - CUS04 - - - - - Since there was no difference in the toxicity of Cutrine®-Ultra and Cu to postfertilization stage eggs, we conclude that Cutrine®-Ultra has a mode of toxicity upon gamete viability or the fertilization physiology other than that associated with Cu exposure. Literature TRVs for D. polymorpha are scarce, although Fisher et al. (1994) determined LCso values for six molluscides and concluded that larvae were more sensitive than adults to five of the compounds. The only Cu-related D. polymorpha information that these authors are aware ofis unpublished by McMahon and Tsou (1990) from a conference proceeding, indicating that 5 mg CulL 100 % mortality of D. polymOlpha veliger in 24 h in unknown water quality conditions. Based on results from the current study, such levels of mortality can be achieved at much lower concentrations ofCu in Lake Ossawinnamakee water. Results for LC99 data are summarized in Table 3. LC99 values are shown in bold type, and 95 % confidence limits in parentheses where the model allowed this estimation. All data are mg CulL. 15

Expt Chemical 1 2 3 Table 3. Summary ofLC99 values. Cutrine 0.081 Pre-fert Post-fert 24-h 48-h 72-h LC99 LC99 LC99 LC99 LC99 0.027 0.039 (0.018 - 0.189) (0.019 - 17.414) 0.038 0.047 CUS04 - - 0.0207 - 0.020 Cutrine - - - - - CUS04 0.016 (0.010 - 0.054) 0.014 - - - Cutrine 0.147 - - - - CUS04 - - - - - Estimates of LC99 from all experiments demonstrate that 24-h exposure with Cutrine®-Ultra can be expected to control the majority ofD. polymOlpha larvae at alllife stages in the range 0.027 - 0.147 mg CulL, using the water quality parameters for Lake Ossawinnamakee in June. 16

Expt Chemical<br />

1<br />

2<br />

3<br />

Table 3. Summary ofLC99 values.<br />

Cutrine 0.081<br />

Pre-fert Post-fert 24-h 48-h 72-h<br />

LC99 LC99 LC99 LC99 LC99<br />

0.027 0.039<br />

(0.018 - 0.189) (0.019 - 17.414)<br />

0.038 0.047<br />

CUS04 - - 0.0207 - 0.020<br />

Cutrine - - - - -<br />

CUS04<br />

0.016<br />

(0.010 - 0.054)<br />

0.014 - - -<br />

Cutrine 0.147 - - - -<br />

CUS04 - - - - -<br />

Estimates of LC99 from all experiments demonstrate that 24-h exposure with<br />

Cutrine®-Ultra can be expected to control the majority ofD. polymOlpha larvae at alllife<br />

stages in the range 0.027 - 0.147 mg CulL, using the water quality parameters for Lake<br />

Ossawinnamakee in June.<br />

16

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