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Consultant's Report - Minnesota State Legislature

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Figure 2 demonstrates that hatching and survival rates from 0 - 24 h were low (7<br />

%). Survival from 24 96 h was much higher, particularly between 72 - 96 h, when<br />

survival was 97 %. The biological half-life for 72- to 96-h-old larvae was 102.2 h, which<br />

was close to the range of 36.0 - 67.2 presented by Fisher et at. (1994) for laboratoryspawned<br />

D. potymorpha of the same age. Mortality beyond 100 h is observed due to<br />

larvae no longer feeding on yolk sacs and subsequently requiring micro-algae food.<br />

Background control mortality was accounted for in the subsequent toxicity tests by using<br />

Abbott's con-ection within the ToxCalc© software package (Tidepool Scientific,<br />

McKinleyville, CA).<br />

10

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