Identification of important interactions between subchondral bone ...
Identification of important interactions between subchondral bone ...
Identification of important interactions between subchondral bone ...
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CHAPTER 7: PAPER IV<br />
Aggrecan degradation in normal, cytokine-induced, and OA cartilage<br />
In both bovine and human OA cartilage, the endogenous proteinases activated by MMP-buffer,<br />
degraded the aggrecan in to fragments that were able to be detected by the biomarker, AGNx-II.<br />
The MMP inhibitor, GM6001, was able to attenuate the levels <strong>of</strong> AGNx-II in both bovine and<br />
human OA cartilage (Table 1). The bovine cartilage pre-cultured with cytokines for 4 and 11<br />
days before metabolic inactivation and incubation in MMP buffer, increased the levels <strong>of</strong> AGNx-<br />
II compared to normal bovine cartilage (Table 1). Furthermore, the human OA cartilage had<br />
greatly higher levels <strong>of</strong> AGNx-II than the bovine samples (Table 1). The cysteine protease<br />
inhibitor, E64, did not affect the normal or pre-cultured bovine samples, whereas E64 increased<br />
the levels significantly in the human OA samples (Table 1).<br />
The proteinases activated by the cysteine buffer in both bovine and human cartilage, also<br />
degraded the aggrecan in to fragments that were able to be detected by the biomarker, AGNx-II.<br />
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