Identification of important interactions between subchondral bone ...
Identification of important interactions between subchondral bone ...
Identification of important interactions between subchondral bone ...
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CHAPTER 4<br />
CHAPTER 4: PAPER I<br />
PAPER I<br />
___________________________________________________________________________<br />
Introductory remarks<br />
PAPER I validates a novel ex vivo model that allows investigation <strong>of</strong> cartilage and <strong>bone</strong> in one<br />
closed system. The development and validation <strong>of</strong> the novel ex vivo model, included selection and<br />
optimization processes that have not been included in PAPER I:<br />
Selection <strong>of</strong> femur head as the explant sample (the embryonic tibia was tested first)<br />
Sex <strong>of</strong> the mice<br />
Time <strong>of</strong> culture period<br />
Selection <strong>of</strong> conditioned medium<br />
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