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Weibel–Palade bodies 749 wheal-and-flare reaction
Immunofluorescence of renal glomerulus in Wegener’s granulomatosis.
Gerald Bertram Webb.
Weibel–Palade bodies
P-selectin granules found in endothelial cells. P-selectin is
translocated rapidly to the cell surface following activation
of an endothelial cell by such mediators as histamine
and C5a.
Weil–Felix reaction
A diagnostic agglutination test in which Proteus bacteria
are agglutinated by the sera of patients with typhus. The
reaction is based upon the cross reactivity of the carbohydrate
antigen shared by Rickettsiae and selected Proteus
strains. Various rickettsial diseases may be diagnosed based
on the reaction patterns of antibodies in the sera of patients
with rickettsial disease with O-agglutinable strains of
Proteus OX19, OX2, and OX12.
Wells, Harry Gideon
A professor of pathology at the University of Chicago who
wrote an important early book in the 1920s titled Chemical
Aspects of Immunity.
Capillary action
Gel with
separated
Ag fragments
Nitrocellulose
Harry Gideon Wells.
Filter paper
Add Ab and
radiolabelled conjugate
Peptides transferred
to nitrocellulose (blot)
Autoradiograph
develop and fix
autoradiograph
Western blot.
Nitrocellulose
Filter paper wick
Buffer
Ag band
western blot (immunoblot)
A method to identify antibodies against proteins of precise
molecular weight. It is widely used as a confirmatory test
for HIV-1 antibody following screening via the ELISA
assay. Following separation of proteins by one- or twodimensional
electrophoresis, they are blotted or transferred
to a nitrocellulose or nylon membrane followed by exposure
to biotinylated or radioisotope-labeled antibody; the antigen
under investigation is revealed by either a color reaction or
autoradiography, respectively.
wheal-and-flare reaction
An immediate hypersensitivity, IgE-mediated human reaction
to an antigen. Application of antigen by a scratch test in
a hypersensitive individual may be followed by erythema (red
flare) and edema (wheal). Atopic subjects whose allergies
have hereditary components experience the effects of histamine
and other vasoactive amines released from mast cell
granules following cross linking of surface immunoglobulin
E (IgE) molecules by antigen or allergen. Refer to urticaria.
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