Untitled - D Ank Unlimited

etrovirus 621 reverse transcriptase
RNA genome
Protease
Integrase
retroviral oncogene capable of transforming host cells following
infection.
retrovirus
A reverse transcriptase- and integrase-containing virus that
permits synthesis of a DNA copy of the viral RNA genome
and its incorporation into host cell DNA. Includes human
immunodeficiency virus (HIV) and human T cell leukemia
virus; an RNA virus that can insert and efficiently express
its own genetic information into a host cell through transcription
of its RNA into DNA and subsequent integration
into the genome of the host cell. Retroviruses are employed
in research to deliberately insert foreign DNA into a cell;
thus, they have the potential for use in gene therapy when
a host cell gene is missing or defective. Retroviruses have
been used to tag tumor-infiltrating lymphocytes in experimental
cancer treatment.
retrovirus immunity
Refer to human immunodeficiency virus and AIDS.
rev protein
A regulatory protein of the human immunodeficiency virus
(HIV) rev gene that facilitates passage of viral transcripts from
a host cell nucleus to the cytoplasm during HIV replication.
reverse anaphylaxis
Anaphylaxis produced by the passive transfer of serum
antibody from a sensitized animal to a normal untreated
recipient only after the recipient was first injected with the
antigen. Thus, the usual order of administration of antigen
and antibody is the reverse of classic anaphylaxis.
reverse genetics approach
The determination of a gene’s role in a particular disease by
investigating a diseased animal’s genome and identifying
sustained mutations in its gene.
reverse immunology
Use of computerized algorithms to predict the likelihood of
a particular mutation resulting in a strong antigen. Several
mutant proteins and peptides have been used to examine the
possibility of inducing tumor-specific immunity.
Retrovirus.
Receptor-binding
Transmembrane
Envelope
Matrix
Capsid
Reverse
transcriptase
reverse Mancini technique
Refer to reverse radioimmunodiffusion.
reverse passive Arthus reaction
A reaction that differs from a classic Arthus reaction only
in that precipitating antibody is injected into an animal
intracutaneously and, after an interval of 30 minutes to 2
hours, the antigen is administered intravenously. Antigen
rather than antibody diffuses from the blood into the tissues,
and antibody rather than antigen diffuses into the
tissue where it encounters and interacts with antigen, with
the consequent typical changes in the microvasculature and
tissues associated with the Arthus reaction.
reverse passive cutaneous anaphylaxis (RPCA)
A passive cutaneous anaphylaxis assay in which the order of
antigen and antibody administration is reversed; the antigen is
injected first, followed by the antibody. In this case, the antigen
must be an immunoglobulin that can fix to tissue cells.
reverse plaque assay
A method to identify antibody-secreting cells regardless of
their antibody specificity. The antibody-forming cells are
suspended in agarose and incubated at 37°C in Petri plates
with sheep red cells coated with protein A. Anti-Ig and
complement are also present. Cells synthesizing and secreting
immunoglobulin become encircled by Ig–anti-Ig complexes
and then link to protein A on erythrocyte surfaces,
leading to hemolytic plaques (zones of lysis). Any class of
immunoglobulin can be identified by this technique through
the choice of the appropriate antibody.
reverse radioimmunodiffusion
A technique to quantify antibody levels that varies from
the single radial diffusion test in only one detail: samples
are placed in gel containing antigen, and as diffusion takes
place, the precipitation rings produced are directly proportional
to the antibody concentration.
reverse transcriptase
An enzyme that is a critical component of retroviruses. It
translates the RNA genome into DNA before integration
R