Untitled - D Ank Unlimited

adioimmunodiffusion test 609 radiolabeling
of the results when read by autoradiography. Refer to single
radial immunodiffusion.
radioimmunodiffusion test
Refer to single radial immunodiffusion.
radioimmunoelectrophoresis
A type of immunoelectrophoresis that employs radiolabeled
antibody or antigen to identify individual precipitin
arcs by autoradiography.
radioimmunoprecipitation assay (RIPA)
A method that demonstrates antibodies against viral
constituents. Virus grown in culture in the presence of
radioactive amino acid is disrupted and incubated with a
test sample that may contain antibodies specific for viral
antigens. This is followed by polyacrylamide gel electrophoresis
of the immunoglobulins in the test sample.
radioimmunoscintigraphy
The use of radiolabeled antibodies to localize tumors or
other lesions through radioactivity scanning after injection
in vivo.
radioimmunosorbent test (RIST)
A solid phase radioimmunoassay to determine serum
immunoglobulin E (IgE) concentration. A standard quantity
of radiolabeled IgE is added to the serum sample to be
cpm
Immunoglobulin
CONTROL
+ +
+
Labeled Ag
+
Labeled and
unlabeled Ag
75% = Amount labeled
IgE added to test
Radioimmunoassay (RIA).
All Ag bound
Labeled IgE (IgE standard)
Labeled IgE
+
Half the labeled
Ag remains free
assayed. The mixture is then combined with Sephadex ® or
dextran beads coated with antibody to human IgE. After
incubation and washing, the quantity of radiolabeled IgE
bound to the beads is measured. The patient’s IgE competes
with the radiolabeled IgE or antibody attached to the beads.
The decrease in labeled IgE attached to the beads compared
to a control, in which labeled IgE combines with the beads
without competition, represents the IgE concentration. The
radioallergosorbent test by comparison assays IgE levels
reactive with a specific allergen.
radioimmunotherapy (RIT)
Treatment of cancer with radioimmunoisotopes.
radiolabeling
Radioisotope incorporation into macromolecules has
increased the sensitivity of immunoassays 30,000-fold.
Radioisotopes permit the detection and quantification of
picogram quantities of an antigen or receptor. Radiolabeled
antibodies can detect primary binding of antibody to
antigen, providing sensitivity and measurements of affinity.
They may be used to identify a tumor site or the presence
and topography of molecules in a cell membrane.
Radiolabeled antigens demonstrated that B cells bind antigen
via cell surface immunoglobulin receptors.
cpm
Anti-IgE IgE to be tested
Radioimmunosorbent test (RIST).
TEST
Test sample
R