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palivizumab (injection) 551 PAP (peroxidase–antiperoxidase) technique
antibody that fixes complement in the cold and lyses red
blood cells at 37°C.
palivizumab (injection)
A monoclonal antibody used for the prevention of serious
lower respiratory tract disease induced by respiratory
syncytial virus (RSV) in pediatric patients at high risk for
RSV disease. Manifests neutralizing and fusion-inhibitory
activity against RSV, as revealed by RSV replication in
laboratory studies. Palivizumab neutralized a panel of 57
clinical RSV isolates.
PAP (peroxidase–antiperoxidase) technique
A method for immunoperoxidase staining of tissue to
identify antigens with antibodies that employs unlabeled
antibodies and a PAP reagent. The same PAP complex may
be used for dozens of different unlabeled antibody specificities.
If the primary antibody against the antigen sought
is made in rabbits, then tissue sections treated with this
reagent are exposed to sheep antirabbit immunoglobulin
followed by the PAP complex. For human primary
Plasma cells decorated with antibody.
Chromogranin.
Prolactin staining in pituitary.
Tissue
Ag +
Rabbit
primary
antibody
Sheep antirabbit immunoglobulin
(linking antibody)
(in excess)
antibody, an additional step must link the human antibody
to the rabbit sandwich technique. Paraffin-embedded tissue
sections are first treated with xylene; after deparaffinization,
they are exposed to hydrogen peroxide to destroy the
endogenous peroxidase. Sections are next incubated with
normal sheep serum to suppress nonspecific binding of
immunoglobulin to tissue collagen. Primary rabbit antibody
against the antigen to be identified is combined with
the tissue section. Unbound primary antibody is removed
by rinsing the sections that are then covered with sheep
antibody against rabbit immunoglobulin. This linking
antibody will combine with any primary rabbit antibody in
+
Peroxidase Peroxidase
Peroxidase-antiperoxidase
(PAP)
Development in chromogenic hydrogen donor and hydrogen peroxide.
(The reaction product is seen as a reddish brown or brown granular deposit
depending upon the chromogenic hydrogen donor used.)
Insulin in β cells.
P