Untitled - D Ank Unlimited

linked recognition 455 linked recognition
FL2
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0
List-Mode Data
FSC SSC FL1 FL2
Event 1 30 60 638 840
Event 2 100 160 245 85
Event 3
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300 650 160 720
840 800
600
400
200
85
0
0 200 400 600 8001000
0 200 400 600 8001000
FL1
FL1
245 638
Useful information can be obtained with a dot plot by determining the
percentage for each population.
10 4
10 3
10 2
10 1
10 0
10 0
3-D Plot
Density Plot
10 1
10 2
10 3
10 4
FL2
Charging electrode
Sample injection tube
Sheath tube
Vent tube
Multiview Plot
FSC
Deflection
plates
SSC
Collection tubes
Particles can be isolated by charging them after they are passed through a
laser. Depending on the charge, the particular will travel to the left or right
sort tube, repelled by or attracted toward the charge plate. All noncharged
particles travel to waste.
10 2
FL3
FL2
FL1
Contour Plot
The different plots can be used to clearly display and analyze populations
of interest.
10 4
10 3
10 2
10 1
10 0
10 0
10 1
10 3
10 4
SSC-Height
SSC-Height
250
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50
0
0
R1
50 100 150 200 250
FSC-Height
CD19 PE
10 4
10 3
10 2
10 1
100 100 103 102 10
CD3 FITC
1
Conventional method for identifying lymphocyte subsets is light scatter
gating. The lymphocytes are gated, markers are set using a two-color
isotype control, then subsequent immunofluorescence analyses of the
remaining files are completed.
250
200
150
100
50
100 0
10 1
10 2
R3
10 3
CD45 PerCP
10 4
CD4 PE
10 0
10 1
10 2
CD3 FITC
Unlike traditional methods of light scatter gating in which lymphocyte
gate purity and recovery are concerns, TriTEST allows the CD45 + lymphocyte
population to be gated, providing unambiguous identification.
Fluorescence
detector
Laser beam
Cells labeled
with fluorescent Ab
10 4
10 3
10 2
10 1
10 0
Light scatter.
+ –
Cells sorted
10 3
Forward-angle
light scatter
detector
Charged
deflection
plates
10 4
10 4
L