Untitled - D Ank Unlimited

linked recognition 454 linked recognition
CD4 FITC
CD8 PE
104 103 102 10
CD3 APC
1 100 disequilibrium in the HLA system of humans is the occurrence
on the same chromosome of HLA-A3 and HLA-B7 in
the Caucasian American population.
10 4
10 3
10 2
10 1
10 0
10 4
10 3
10 2
10 1
10 0
104 103 102 10
CD3 FITC
1 100 CD8 PE
10 4
10 3
10 2
10 1
10 0
104 103 102 10
CD3 APC
1 100 Three bivariate plots displaying stained lymphocytes. CD3 + cells are stained with APC, CD4 + cells are stained with FITC, and CD8 + cells are stained
with PE.
H 2 N
N + 1 –
(CH 2 ) 3
CH 2
N +
1 –
NH 2
CH 2
CH 4
CH 2
CH 3
CH 4
Structure Bound to DNA
DNA content can be quantified by the use of propidium iodide (PI) fluorescent dye. The dye intercalates between the base pairs to stain double-stranded
nucleic acids. The amount of DNA that PI binds is proportional to the DNA content.
Counts
250
200
150
100
50
0
0
200
G 0 /G 1
S
G 2 /M
400
600
FL2-Area
800
1000
Staining DNA with PI and analyzing the sample by flow cytometry
permits the percentage of cells in each phase of the cell cycle to be
determined.
Longpass
460 500 540
Shortpass
460 500 540
Bandpass
460 500 540
LP 500 SP 500 BP 500/50
The specificity of an optical detector for a particular fluorescent dye is
optimized by placing a filter in front of the detector that allows a narrow
range of wavelengths to pass through the filter.
Laser
Laser
Laser
Time
linked recognition
The need for helper T cells and B cells participating in the
antibody response to a thymus-dependent antigen to interact
with different epitopes linked physically in the same
antigen. Epitopes that B cells and helper T cells recognize
must be linked physically for the helper T cell to activate
the B cell, which constitutes linked recognition. The need
for hapten and carrier to be linked physically rather than
merely mixed together to stimulate a secondary response to
the hapten.
Voltage
Voltage
Voltage
Time
Time
A pulse is created when a particle enters the laser beam and starts to scatter
light. The highest point of the pulse occurs when the particle is in the
center of the beam and the maximum amount of scatter is achieved. As
the particle leaves the laser, the pulse returns to baseline.