Untitled - D Ank Unlimited

immunophilins (Tacrolimus and Rapamycin sirolimus) 382 immunoproliferative small intestinal disease (IPSID)
Tissue
Ag
+
Rabbit
primary
antibody Biotinylated
secondary antibody
(e.g., goat antirabbit)
immunophilins (Tacrolimus and Rapamycin sirolimus)
High-affinity receptor proteins with peptidyl-prolyl cis–trans
isomerase activity that combine with such immunosuppressants
as cyclosporin A, FK506, and rapamycin. They prevent
the activity of rotamase by blocking conversion between
cis and trans rotamers of the peptide and protein substrate
peptidyl–prolylamide bond. Immunophilins are important
in transducing signals from the cell surface to the nucleus.
Immunosuppressants have been postulated to prevent signal
transduction mediated by T lymphocyte receptors which
blocks nuclear factor activation in activated T lymphocytes.
Cyclophilin- and FK506-binding proteins represent immunophilins.
Drug–immunophilin complexes are implicated in
the mechanism of action of the immunosuppressant drugs,
cyclosporin, FK506, and rapamycin.
immunophysiology
The physiologic basis of immunologic processes.
immunopotency
The capability of a part of an antigen molecule to function
as an epitope and induce the synthesis of specific antibodies.
+
ABC COMPLEX
Reagent A Reagent B
Avidin DH
(in excess)
Biotinylated
peroxidase H
AVIDIN
(with 4 binding
sites for biotin)
BIOTIN
PEROXIDASE
Immunoperoxidase method. Development in chromogenic hydrogen donor and hydrogen peroxide. (The reaction product is seen as a reddish brown or
brown granular deposit depending upon the chromogenic hydrogen donor used.)
immunopotentiation
Facilitation of the immune response usually with the aid
of adjuvants such as muramyl dipeptide, Freund’s adjuvant,
synthetic polynucleotides, or other agents. Biological
response modifiers, cloned cytokines, and purified immunoglobulins
have been used as immunopotentiating agents.
immunoprecipitation
A method to recover or isolate an antigen molecule from
solution by uniting it with an antibody and rendering the
antigen–antibody complex insoluble through interaction with
an anti-antibody or through coupling the first antibody to an
insoluble support such as a particle or bead. A common technique
used for identifying protein–protein interactions. IP is
usually performed using a primary antibody together with
a second antibody resin or protein A or G resin. After IP, a
western blot is used to detect any possible proteins associated
with the target protein. Used to isolate or detect antigens
present in complex mixtures of proteins in solution.
immunoproliferative small intestinal disease (IPSID)
Mediterranean lymphoma α heavy chain disease. A varied
group of disorders in which there is monoclonal synthesis of