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fluorescein-labeled antibody 278 fluorescence quenching
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appear apple-green under ultraviolet irradiation, permitting
easy detection of antigens of interest in tissues and cells.
FITC fluoresces at 490 and 520 nm. FITC-labeled antibodies
are useful for the demonstration of immune deposits in
both skin and kidney biopsies.
fluorescein-labeled antibody
An antibody tagged with a fluorescein derivative such as
fluorescein isothiocyanate. These antibodies are useful for
localizing antigens in tissues and cells by their brilliant
apple-green fluorescence under ultraviolet light.
fluorescence
Emission of light of one wavelength by a substance irradiated
with light of a different wavelength.
fluorescence-activated cell sorter (FACS)
An instrument designed to measure the size, granularity,
and fluorescence of cells attributable to bound fluorescent
antibodies as individual cells flow in a stream
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Fluid mosaic model.
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Structure of fluorescein.
RBC debris
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CD45 ECD
Granulocytes
Monocytes
Lymphocytes
Flow cytometric analysis of normal peripheral blood.
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Structure of fluorescein isothiocyanate (FITC).
past photodetectors. Single-cell analysis by this method
is termed flow cytometry, and the device that makes the
measurements and/or sorts cells is termed a flow cytometer
or cell sorter. The flow cytometry fluorescence detectors are
connected to computer-controlled electromagnetic deflector
plates programmed to deposit a cell with a particular fluorescent
signal due to bound fluorochrome-tagged antibody
of a particular wavelength (color) and intensity to a specific
collection tube.
fluorescence enhancement
Increased fluorescence of certain substances after their
combination with antibody. This is attributable to changing
the substance from an aqueous milieu to the hydrophobic
surroundings of the antibody combining site.
fluorescence microscopy
A special microscope that uses ultraviolet light to illuminate
a tissue or cell stained with a fluorochrome-labeled substance
such as an antibody against an antigen of interest in the tissue.
When returning from an excited state to a ground state,
the tissue emits fluorescent light that permits the observer to
localize an antigen of interest in the tissue or cell.
fluorescence quenching
A method to ascertain association constants of antibody
molecules interacting with ligands. It results from excitation