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Flt3 ligand 277 fluorescein isothiocyanate (FITC)
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Negative flow crossmatch.
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Positive flow crossmatch.
for numerous very different, highly specialized assays. It is
a multifactorial analysis technique and provides the capability
for performing many of these assays simultaneously.
Flt3 ligand
A hematopoietic growth factor significant in NK cell development.
It is related to the macrophage colony-stimulating
factor, which binds to a receptor termed fms. Thus Flt3 is a
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receptor termed fms-like tyrosine kinase 3, and Flt3-L is its
ligand. Flt3-L is synthesized by bone marrow stromal cells.
fludarabine
A nucleoside precursor that becomes a purine analogue
known as F-ara-A following injection into a human or
other animal. F-ara-A enters and accumulates in cells in a
phosphorylated form (F-ara-Appl), which inhibits cell proliferation
mainly by blocking ribonucleotide reductase and
DNA polymerase. Its incorporation into DNA inhibits DNA
ligase, while incorporation into RNA blocks transcription.
An anticancer drug used to treat low-grade leukemias and
lymphomas, it has been used in transplantation to facilitate
graft acceptance when used in combination with low doses of
other immunosuppressive drugs. Toxicity is relatively low.
fluid mosaic model
A fluid lipid molecular bilayer in the plasma membrane and
organelle membranes of cells. This structure permits membrane
proteins and glycoproteins to float. The lipid molecules
are situated in a manner that arranges the polar heads toward
the outer surfaces and their hydrophobic side chains project
into the interior. There can be lateral movement of molecules
in the bilayer plain or they may rotate on their long
axes. This is the Singer–Nicholson fluid mosaic. The bilayer
consists of glycolipids and phospholipids. Amphipathic lipids
and globular proteins are spaced throughout the membrane.
The fluid consistency permits movement of the receptors,
proteins, and glycoprotein laterally.
FluMist ®
Contains live attenuated influenza viruses that reproduce in
the nasopharynx of the recipient and are present in respiratory
secretions.
fluorescein
A yellow dye that stains with brilliant apple-green fluorescence
when excited. Its isothiocyanate derivative is widely employed
to label proteins such as immunoglobulins that are useful in
diagnostic medicine as well as in basic science research.
fluorescein isothiocyanate (FITC)
A widely used fluorochrome for labeling antibody
molecules. It may also be used to label other proteins.
Fluorescein-labeled antibodies are popular because they
Flow cytometry is a fast, accurate way to measure multiple characteristics of a single cell simultaneously. These objective measurements are made one cell
at a time at routine rates of 500 to 4000 particles per second in a moving fluid stream. A flow cytometer measures relative size (FSC), relative granularity
or internal complexity (SSC), and relative fluorescence. Three-color flow cytometry is used to analyze blood cells by size, cytoplasmic granularity, and
surface markers labeled with different fluorochromes.
SSC
FSC
Orange
Green
FSC
SSC
Green
Orange
Red
F