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CD15 (Leu M1) 143 CD20 primary antibody
CD15 (Leu M1)
A monoclonal antibody that recognizes the human myelomonocytic
antigen lacto-N fucopentose III. It is present
on more than 95% of mature peripheral blood eosinophils
and neutrophils and is present at low density on circulating
monocytes. In lymphoid tissue, CD15 reacts with Reed–
Sternberg cells of Hodgkin disease and with granulocytes.
It also reacts with few tissue macrophages and does not
react with dendritic cells.
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γ/ζ
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Structure of CD16.
CD16
An antigen that is also known as the low-affinity Fc
receptor for complexed IgG–FcγRIII. It is expressed on
natural killer (NK) cells, granulocytes, neutrophils, and
macrophages. Structural differences in the CD16 antigen
from granulocytes and NK cells have been reported. This
apparent polymorphism suggests two different genes for the
FcγRIII molecule in polymorphonuclear leukocytes (PMNs)
and in NK cells. The CD16 molecule in NK cells has a
transmembrane form, whereas it is phosphatidylinositol
(PI)-linked in granulocytes. CD16 mediates phagocytosis. It
is the functional receptor structure for performing antibodydependent,
cell-mediated cytotoxicity (ADCC). CD16 is
also termed FcγRIII.
CDw17
An antigen that is a cell surface glycosphingolipid moiety,
lactosylceramide, and is present principally on granulocytes
but also on monocytes and platelets.
CD18
An antigen (molecular weight of 95 kDa) that is an integral
membrane glycoprotein. This integrin β 2 subunit is noncovalently
linked to CD11a, CD11b, and CD11c molecules and
is expressed on leukocytes. It is important for cell adhesion.
The immunodeficiency known as leukocyte adhesion
deficiency (LAD) has been shown to be caused by a genetic
defect of the CD18 gene.
CD19
A constituent of the B cell coreceptor.
CD20
A B cell marker (molecular weight of 33, 35, and 37 kDa) that
appears relatively late in B cell maturation (after the pro-B
cell stage) and persists for some time before the plasma cell
stage. Its molecular structure resembles that of a transmembrane
ion channel. The gene is on chromosome 11 at band
q12–q13. It may be involved in regulating B cell activation.
CD20 primary antibody
A mouse monoclonal antibody (clone L26) directed against
an intracellular epitope of the CD20 antigen present on
human B lymphocytes. It may be used to aid in the identification
of cells of B lymphocytic lineage and is intended
for qualitative staining in sections of formalin-fixed,
paraffin-embedded tissue. Anti-CD20 antibodies specifically
bind to antigens located in the plasma membrane and
cytoplasmic regions of normal B lymphocytes that may also
be expressed in Reed–Sternberg cells. Unexpected antigen
expression or loss of expression may occur, especially in
neoplasms. Occasional stromal elements surrounding heavily
stained tissue and/or cells may show immunoreactivity.
The clinical interpretation of any staining, or its absence,
must be complemented by morphological studies and evaluation
of proper controls.
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Structure of CD19.
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Structure of CD20⎯tonsil.
CD20 primary antibody.
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