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HEAVY METALS BIOSORPTION FROM AQUEOUS SOLUTION BY ...

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88<br />

Sławomir Wierzba<br />

The experiment results of Cu(II) and Zn(II) biosorption and desorption are reported in<br />

Table 1. Desorption efficiency of Cu(II) and Zn(II) by living cells was 76.3 and 68.4%<br />

under 0.1 M HCl and it was 93.1 and 86.5% by non-living cells, respectively. It indicates<br />

that 0.1 M HCl can effectively desorbs the bound Cu(II) and Zn(II) from non-living cells,<br />

but not very effective for living cells. And the desorption of Cu(II) is more effective than<br />

that of Zn(II). It also may be due to the living cells uptake a part of Cu(II) and Zn(II)<br />

through intracellular accumulation, since it is possible to remove metals from cell surfaces<br />

after biosorption but not bioaccumulation [6].<br />

Conclusions<br />

Results of this study demonstrated that the binding capacity of living cells is<br />

significantly higher than that of non-living cells at tested conditions. It was also found that<br />

0.1 M HCl can effectively desorb the bound Cu(II) and Zn(II) from non-living cells, but not<br />

very effective for living cells. The results of biosorption time and desorption experiments<br />

suggested that Cu(II) and Zn(II) uptake by the living cells might be enhanced by<br />

intracellular accumulation.<br />

The results suggest that Pseudomonas sp. G1 is a potential adsorbing media for metals<br />

in the treatment of wastewater containing Cu(II) and Zn(II). So this bacterium may be<br />

employed for metal remediation in simple reactors or even in situ.<br />

References<br />

[1] Gabr R.M., Hassan S.H.A. and Shoreit A.A.M.: Biosorption of lead and nickel by living and non-living cells<br />

of Pseudomonas aeruginosa ASU 6a. Int. Biodeter. Biodegr., 2008, 62, 195-203.<br />

[2] Vijayaraghavan K. and Yun Y-S.: Bacterial biosorbents and biosorption. Biotechnol. Adv., 2008, 26,<br />

266-291.<br />

[3] Choi A., Wang S. and Lee M.: Biosorption of cadmium, copper, and lead from aqueous solution by<br />

Ralstonia sp. and Bacillus sp. isolated from diesel and heavy metal contaminated soil. Geosci. J., 2009,<br />

13(4), 331-341.<br />

[4] Chang J-S., Law R. and Chang Ch-Ch.: Biosorption of lead, copper and cadmium by biomass of<br />

Pseudomonas aeruginosa PU21. Water Res., 1997, 31(7), 1651-1658.<br />

[5] Lopez A., Lazaro N., Priego J.M. and Marques A.M.: Effect of pH on the biosorption of nickel and other<br />

heavy metals by Pseudomonas fluorescens 3F39. J. Ind. Microbiol. Biot., 2003, 24, 146-151.<br />

[6] Chen C.X., Wang P.Y., Lin Q., Shi Y.J., Wu W.X. and Chen Y.X.: Biosorption of copper(II) and zinc(II)<br />

from aqueous solution by Pseudomonas putida CZ1. Colloid. Surface B., 2005, 46, 101-107.<br />

[7] Liu Y., Cao Q., Luo F. and Chen J.: Biosorption of Cd 2+ , Cu 2+ , Ni 2+ , and Zn 2+ ions from aqueous solutions by<br />

pretreated biomass of brown alge. J. Hazard. Mater., 2009, 163, 931-938.<br />

[8] Hawari A.H. and Mulligan C.N.: Biosorption of lead(II), cadmium(II), copper(II) and nikel(II) by anaerobic<br />

granular biomass. Bioresour. Technol., 2006, 97, 692-700.

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