Magnetic Separation: Industrial and Lab Scale Applications

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Figure 13. Examples of batch magnetic separators applicable for magnetic separation of proteins and peptides. A: Dynal MPC -S for six microtubes (Dynal, Norway); B: Dynal MPC – 1 for one test tube (Dynal, Norway); C: Dynal MPC – L for six test tubes (Dynal, Norway); D: magnetic separator for six Eppendorf tubes (New England BioLabs, USA); E: MagneSphere Technology Magnetic Separation Stand, two position (Promega, USA); F: MagnaBot Large Volume Magnetic Separation Device (Promega, USA); G: MagneSphere Technology Magnetic Separation Stand, twelve-position (Promega, USA); H: Dynal MPC – 96 S for 96-well microtitre plates (Dynal, Norway); I: MagnaBot 96 Magnetic Separation Device for 96-well microtitre plates (Promega, USA); J: BioMag Solo-Sep Microcentrifuge Tube Separator (Polysciences, USA); K: BioMag Flask Separator (Polysciences, USA); L: MagneSil Magnetic Separation Unit (Promega, USA); M: MCB 1200 processing system for 12 microtubes based on MixSep process (Sigris Research, USA); N: PickPen magnetic tool (Bio-Nobile, Finland). Reproduced with the permission of the above mentioned companies; the photos were taken from their www pages. (REF: Safarik 2004 Protein Review) In an excellent review, Safarik and Safarikova discuss advantages and the equipment for a successful protein purification via magnetic means with a full scan of magnetic separation applications in isolation of enzymes, antibodies and proteins (Safarik 2004 Biomag Res Tech). The efforts for the industrial scale applications are noteworthy and can be applied for a few biological molecules (Hubbuch 2002 BiotechBioeng, Safarik 2001 BiotechLett).
Magnetic separation based protein analysis and detection systems on chips are of great interest for early diagnosis for fatal infections. Biobarcoded magnetic beads (Nam 2003 Science), microfluidic biochemical detection system (Choi 2002 LabOnaChip), micromachined magnetic particle separator (Ahn 1996) are prominent examples of this field. Recently, nanorods of Ni with Au edges were successfully used to remove His-tagged proteins with 90% recovery (Lee 2004 Angewandte). c. Cell separation Similar to protein purifications, magnetic separations offer rapid quantification, high cell recovery when compared to the conventional methods, i.e. centrifugation (Chang 2005 J Ind Microbiol). As early as 1977, 99% recovery of neuroblasioma cells was obtained in a matter of minutes (Kronick 1977 Science). Same year, magnetic separation of red blood cells and lymphoid cells were also introduced (Molday 1977 Nature). Magnetic separation of cells is advantageous over the conventional methods mainly because it lets target cells to be isolated directly from the medium, i.e. blood, bone marrow, tissue homogenates, stool, cultivation, media, food, water, soil etc (Safarik 1999 JChromB).
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Magnetic Separation: Industrial and Lab Scale Applications

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