Magnetic Separation: Industrial and Lab Scale Applications

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Figure 11. On a single particle, several necessary sections of a magnetic material that could be used in biological systems is summarized. (from ref. [Salata 2004 Review] with permission) b. Protein purification Magnetic separation of biological entities proven to be rapid and more effective for over 30 years now (Robinson 1973 Biotech, Lilly 1974 Biotech, Guesdon 1977 Immunochem, Hirschbein 1982 ApplBiochem, Hubbuch 2001). Proper coating and labeling of the magnetic particles and the target species would yield hassle-free and time-saver purifications with reduced costs (Setchell 1985, Safarik 2004 BiomagResTech). Although very effective, magnetic affinity separations need to be very specific. Immobilization of ligands on the magnetic adsorbents for the capture of the target species is crucial and perhaps because of this, though standard liquid column chromatography is currently the most often used technique, magnetic separations will prevail with significant research in magnetic affinity separations and biochemical analysis (Safarik 2004 BiomagResTEch). Recent studies on magnetic materials for protein separations involve silica coated magnetite with amino functionality for salmon sperm DNA elution (Bruce 2005 Langmuir), phospholipid coated magnetite for myoglobin recovery (Bucak 2003 BiotechnolProg), polyethylenimine coated magnetite for purification of plasmid DNA from bacterial cells (Chiang 2005 J ChromatogB), magnetic separation of erbium (III) attached biological particles (Evans 1981 Science), magnetic polyacrylamide-agarose beads for measuring rabbit antibody (Guesdon 1977),
magnetic polymer latexes for isolation of trypsin from pancreatic extract (Khng 1998), ProtA-immobilized magnetic immunomicrospheres for immunoaffinity purification of antibodies IgG2a from mouse ascites (Liu 2004 JApplPolS), silica coated magnetite with iminodiacetic acid functionality for bovine hemoglobin (BHb) and bovine serum albumin (BSA) (Ma 2006 Coll), streptavidin-functionalized magnetic nanoparticles for stimulant biotoxin (Mertz 2005 JMMM), magnetite and nickel particles for the immobilization of a-chymotrypsin (Munro 1975 IEEE), Nickel-NiO-BSA-chymotrypsin for casein hydrolysis (Munro 1981 BiotechBioeng), magnetic affinity support for adsorption of lysozyme (Tong 2001 BiotechProg), streptavidin-biotin coated magnetic beads for DNA-RNA isolation (Uhlen 1989 Nature), polyethyleneimine coated magnetite for virus capture (Veyret 2005 AnalBiochem), carboxyl-modified magnetic nanobeads for the isolation of genomic DNA from human whole blood (Xie 2004 JMMM), TeNT-linked iron oxide nanobeads with dextran coating for explaining the relative capacity of the specific compartments of a cell resulting from endocytosis through different receptors that promote antigen presentation and immune (Perrin- Cocon 2001 JMMM). Figure 8 describes a standard setup for a bench-top magnetic separation (Tartaj 2003 Review). Figure 9 shows a summary of the available magnetic separators (Safarik 2004 BiomagResTech).
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Magnetic Separation: Industrial and Lab Scale Applications

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