Herbert T. Nagasawa
Herbert T. Nagasawa
Herbert T. Nagasawa
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CURRICULUM VITAE<br />
Name: <strong>Herbert</strong> T. <strong>Nagasawa</strong><br />
Place of Birth: Hilo, Hawaii<br />
Education:<br />
B.S. 1950 Western Reserve University, Cleveland, OH<br />
Major: Chemistry<br />
Minor: Mathematics<br />
Honors: Phi Society (Honorary Scholastic)<br />
Ph.D. 1955 University of Minnesota, Minneapolis, MN<br />
Major: Organic Chemistry<br />
Minor: Analytical Inorganic and Physical Chemistry<br />
Honors: Phi Lambda Upsilon (Chemistry Scholastic)<br />
Post-Doctoral 1955- Department of Biochemistry, University of Minnesota,<br />
Fellow 1957 Minneapolis, MN<br />
Honors: Sigma Xi (Scientific Research)<br />
Military Service:<br />
1945 - 1947 Military Intelligence Service, U.S. Army<br />
Positions Held:<br />
1951 - 1954 Teaching Assistant, Department of Chemistry, University of<br />
Minnesota, Minneapolis, MN<br />
1954 - 1955 Pre-doctoral Fellow, Allied Chemical & Dye Corp., Department of<br />
Chemistry, University of Minnesota, Minneapolis, MN<br />
1955 - 1957 Post-doctoral Fellow, Department of Biochemistry, University of<br />
Minnesota, Minneapolis, MN<br />
1957-1961 Senior Chemist, Radioisotope Service Research Laboratory,<br />
Veterans Administration Medical Center, Minneapolis, MN<br />
1961-1978 Senior Scientist, Laboratory for Cancer Research, Veterans<br />
Administration Medical Center, Minneapolis, MN<br />
1961 - 1976 Principal Scientist, General Medical Research, Veterans<br />
Administration Medical Center, Minneapolis, MN<br />
1961 - 1981 Member and Basic Science Representative, Research and<br />
Education Committee, Veterans Administration Medical Center,<br />
Minneapolis, MN<br />
1978 - 2002 Senior Research Career Scientist, Veterans Administration<br />
MedicalCenter, Minneapolis, MN<br />
2002 - 2004 Senior Medical Research Scientist, Veterans Administration<br />
Medical Center, Minneapolis, MN
1959 - 1963 Assistant Professor, Pharmaceutical Chemistry, University of<br />
Minnesota, Minneapolis, MN<br />
1963 - 1972 Associate Professor, Medicinal Chemistry, University of Minnesota,<br />
Minneapolis, MN<br />
1973 - 2004 Professor, Medicinal Chemistry, University of Minnesota,<br />
Minneapolis, MN (Officially retired 6/04)<br />
1987 - 1995 Professor, Pharmacology, University of Minnesota, Minneapolis,<br />
MN<br />
1990 - 2004 Professor, Toxicology, University of Minnesota, Duluth, MN<br />
(Officially retired 6/04)<br />
2004-2007 Member, Center for Drug Design, Academic Health Center,<br />
University of Minnesota<br />
2007-to date Adjunct Professor, Center for Drug Design, Academic Health<br />
Center, University of Minnesota<br />
1972 - 1984 Associate Editor, Journal of Medicinal Chemistry, American<br />
Chemical Society (Acting Editor: 8/73 to 6/74)<br />
1985 - 2004 Senior Editor, Journal of Medicinal Chemistry, American Chemical<br />
Society (Retired 6/04)<br />
1974 - 1977 Member, Health Sciences Policy and Review Council, University of<br />
Minnesota Graduate School, Minneapolis, MN<br />
1976 - 1978 Chairperson, Biomedical Research Committee, Alcohol and Other<br />
Drub Abuse Program (AODAP), University of Minnesota,<br />
Minneapolis, MN<br />
1983 - 1984 AODAP Biomedical Grants Review Committee, University of<br />
Minnesota, Minneapolis, MN<br />
1980 - 1981 Ad Hoc Member, Initial Review Group (IRG), Biomedical Research<br />
Review Committee, Alcohol, Drug Abuse and Mental Health<br />
Administration (ADAMHA)<br />
1984 - 1985 Member, Special Review Committee, "National Cooperative Drug<br />
Discovery Group (NCDDG)", National Cancer Institute, National<br />
Institutes of Health (NIH)<br />
1985 Judge, Third Annual Graduate Student Awards, Mechanisms<br />
Section, Society of Toxicology<br />
1990 Visiting Professor, College of Pharmacy, Washington State<br />
University, Pullman, WA (March 24-31)<br />
1990 - 1997 Editorial Board, Bioconjugate Chemistry, American Chemical<br />
Society<br />
Professional Societies:<br />
American Academy of Anti-Aging Medicine (A 4 M)<br />
American Chemical Society (ACS)<br />
American Society for Pharmacology and Experimental Therapeutics (ASPET)<br />
American Association for Cancer Research (AACR)<br />
American Association for the Advancement of Science (AAAS)
International Society for Biomedical Research on Alcoholism (ISBRA)<br />
International Society for the Study of Xenobiotics (ISSX)<br />
New York Academy of Sciences (NYAS): Elected as Fellow, 1983<br />
Research Society on Alcoholism (RSA)<br />
Society of Toxicology (SOT)<br />
Nitric Oxide Society<br />
Listed: American Men and Women of Science; Who's Who in the Midwest<br />
Graduate Faculty Appointment:<br />
Medicinal Chemistry: B Appointment (Full Member)<br />
Pharmacology: A Appointment (Associate Member)<br />
Toxicology: B Appointment (Full Member)<br />
Research Interests: Design and synthesis of a) trapping agents for the detoxification of<br />
xenobiotic substances that are activated to toxic metabolites in vivo, b) latentiated<br />
(prodrug) forms of biologically active substances such as nitroxyl (HNO), c) prodrugs of<br />
cysteine and glutathione as protective agents for cellullar oxidative stress, and d)<br />
cyanide antidotes based on β-mercaptopyruvate.
Publications<br />
<strong>Nagasawa</strong>, H. T. Ph.D. Thesis, Studies in Polypeptide Synthesis: Some Polyglycyl<br />
Derivatives of Desoxyephedrine. University of Minnesota 1955.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. On the Acylation of the Carcinogen, 2-<br />
Aminofluorene, by Rat Liver In Vitro. Biochim. Biophys. Acta 1957, 24, 631-632.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. A Note on the Deacylation of the Carcinogen, 2-<br />
Acetamidofluorene and Related Compounds by Rat Liver and Intestine. Biochim.<br />
Biophys. Acta 1957, 25, 186-189.<br />
Gutmann, H. R.; Burtle, J. G.; <strong>Nagasawa</strong>, H. T. Protein Binding of Model Quinone<br />
Imides. I. The Synthesis of Some Fluorenoquinone Imides. J. Am. Chem. Soc. 1958,<br />
80, 5551-5555.<br />
<strong>Nagasawa</strong>, H. T.; Morgan, M. A.; Gutmann, H. R. The Enzymatic Oxidation of o-<br />
Aminophenols. Biochim. Biophys. Acta 1958, 28, 665-666.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. Preparation and Properties of S-Acetyl-Nbenzoylcysteamine.<br />
J. Org. Chem. 1958, 23, 487.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. The Oxidation of o-Aminophenols by Cytochrome c<br />
and Cytochrome Oxidase. I. Enzymatic Oxidations and Binding of Oxidation Products<br />
to Bovine Serum Albumin. J. Biol. Chem. 1959, 234, 1593-1599.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. The Oxidation of o-Aminophenols by Cytochrome c<br />
and Cytochrome Oxidase. III. 2,3-Fluorenoquinone from 2-Amino-3-fluorenol and<br />
Binding of Quinonoid Oxidation Products to Bovine Serum Albumin. J. Biol. Chem.<br />
1960, 235, 3466-3471.<br />
Alexander, C. S.; <strong>Nagasawa</strong>, H. T.; Filbin, D. Disribution and Excretion of<br />
Aminonucleoside-8-C 14 in Normal and Nephrotic Rats. Proc. Soc. Exp. Biol. Med. 1962,<br />
111, 521-526.<br />
Alexander, C. S.; Hunt, V. R.; <strong>Nagasawa</strong>, H. T. Dose-Response Relationship in<br />
Aminonucleoside Nephrosis. Proc. Soc. Exp. Biol. Med. 1963, 112, 506-510.<br />
Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Aminonucleoside of Puromycin: Elimination of<br />
Nephrotoxicity by Acetylation of the Aminoribose Moiety. Biochem. Pharmacol. 1964,<br />
13, 548-551.<br />
<strong>Nagasawa</strong>, H. T.; Osteraas, A. J. The Biological Arylation of Proteins In Vitro by a<br />
Metabolite of the Carcinogen, N-2-Fluorenylacetamide. Biochem. Pharmacol. 1964, 13,<br />
713-723.
Dickie, N.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Inhibition of Nucleic Acid Synthesis in<br />
Escherichia coli B by Puromycin Aminonucleoside. Biochim. Biophys. 1965, 95, 156-<br />
169.<br />
Derr, R. F.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. An Interaction Between Aminopurines,<br />
Aminopyrimidines and Fluorescent Thin-Layer Plates. J. Chromatogr. 1966, 21, 146-<br />
147.<br />
Dickie, N.; Norton, L. F.; Derr, R. F.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. The Effect of<br />
Puromycin Aminonucleoside on the Incorporation of Labeled Precursors into Rat Kidney<br />
RNA. Biochim. Biophys. 1966, 129, 288-293.<br />
Dickie, N.; Norton, L. F.; Derr, R. F.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. The Inhibition<br />
of Adenosine Deaminase by a Metabolite of the Nephrotoxic Drug, Puromycin<br />
Aminonucleoside. Proc. Soc. Exp. Biol. Med. 1966, 123, 421-423.<br />
<strong>Nagasawa</strong>, H. T.; Gutmann, H. R. Ortho-methoxy Derivatives of the Carcinogen, N-2-<br />
Fluorenylacetamide: Latent Biological Arylating Agents. J. Med. Chem. 1966, 9, 719-<br />
725.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A. Synthesis of Ring Homologs of Proline by the<br />
Favorskii Rearrangement of -Halolactams. Tetrahedron Lett. 1966, 44, 5393-5399.<br />
Alexander, C. S.; Swingle, K. F.; <strong>Nagasawa</strong>, H. T. Tetratogenic Effect of Puromycin<br />
Aminonucleoside on Rat Kidney. Nephron 1966, 3, 344-351.<br />
<strong>Nagasawa</strong>, H. T.; Swingle, K. F.; Alexander, C. S. Metabolism of Aminonucleoside-8-<br />
C 14 in the Rat and Guinea Pig. Biochem. Pharacol. 1967, 16, 2211-2219.<br />
Derr, R. F.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. The Metabolism of Puromycin<br />
Aminonucleoside in the Normal, "Pre-nephrotic" and Nephrotic Rat. Proc. Soc. Exp.<br />
Biol. Med. 1967, 125, 248-252.<br />
<strong>Nagasawa</strong>, H. T.; Alexander, C. S.; Swingle, K. F. Inhibition of Aminonucleoside<br />
Nephrosis in Rats. The Lack of Effect of Hepatic Drug Metabolizing Enzyme Inhibitors<br />
and Stimulators on Nephrotoxicity. Toxicol. Appl. 1967, 11, 336-345.<br />
Derr, R. F.; Loechler, D. K.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Inhibition of Rat Liver<br />
Microsomal N-Demethylase by -Naphthylisothiocyanate: Studies with Puromycin<br />
Aminonucleoside. Proc. Soc. Exp. Biol. Med. 1967, 126, 844-845.<br />
Derr, R. F.; Loechler, D. K.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Metabolism of<br />
Aminonucleoside-8-C 14 in the Mouse. The Relationship Between Metabolism and<br />
Experimental Nephrosis. Biochem. Pharmacol. 1968, 17, 265-268.
Derr, R. F.; Loechler, D. K.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Inhibition of<br />
Aminonucleoside Nephrosis in Rats. IV. Prevention of N 6 -Methyladenosine. J. Lab.<br />
Clin. Med. 1968, 72, 363-369.<br />
<strong>Nagasawa</strong>, H. T.; Fraser, P. S.; Elberling, J. A. Chromatographic Properties of Some<br />
Cyclic -Imino Acids Homologous to Proline, and their DNP-, DNS- AND PTH-<br />
derivatives. J. Chromatogr. 1969, 44, 300-306.<br />
<strong>Nagasawa</strong>, H. T.; Alexander, C. S.; Shirota, F. N.; Ghobrial, H.; Swingle, K. F.; Derr, R.<br />
F. Metabolic Basis for the Lack of Nephrotoxicity of Acetylated Puromycin<br />
Aminonucleoside in Rats. Toxicol. Appl. Pharmacol. 1970, 16, 1-9.<br />
Derr, R. F.; Aaker, A.; Alexander, C. S.; <strong>Nagasawa</strong>, H. T. Synergism Between Cobalt<br />
and Ethanol on Rat Growth Rate. J. Nutr. 1970, 100, 521-524.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Fraser, P. S.; Mizuno, N. S. Medium Ring Homologs<br />
of Proline as Potential Amino Acid Antimetabolites. J. Med. Chem. 1971, 14, 501-508.<br />
<strong>Nagasawa</strong>, H. T.; Shirota, F. N.; Alexander, C. S. Identification and Synthesis of the<br />
Major Nucleoside Metabolite in Rabbit Urine After Administration of Puromycin<br />
Aminonucleoside. J. Med. Chem. 1972, 15, 177-181.<br />
<strong>Nagasawa</strong>, H. T.; Fraser, P. S.; Elberling, J. A. N-Phenyl-2-thio-1,2-azetidinecarboximide,<br />
the Phenylthiogydantoin of Azetidine-2-carboxylic Acid. J. Org. Chem.<br />
1972, 37, 516-519.<br />
<strong>Nagasawa</strong>, H. T.; Shirota, F. N.; Matsumoto, H. Decomposition of<br />
Methylazoxylmethanol, the Agycone of Cycasin, in D2O. Nature 1972, 236, 234-235.<br />
Elberling, J. A.; <strong>Nagasawa</strong>, H. T. The Twelve- to Fifteen-Membered Ring Homologs of<br />
Proline. J. Heterocycl. Chem. 1972, 9, 411-414.<br />
<strong>Nagasawa</strong>, H. T.; Kohlhof, J. G.; Fraser, P. S.; Mikhail, A. A. Sythesis of 1-Hydroxy-Lproline<br />
and Related Cyclic N-Hydroxyamino Acids. Metabolic Disposition of 14 C-<br />
Labeled 1-Hydroxy-L-proline in Rodents. J. Med. Chem. 1972, 15, 483-486.<br />
<strong>Nagasawa</strong>, H. T.; Thompson, J. A. Reactions of Interest in Medicinal Chemistry. In<br />
Annual Reports in Medicinal Chemistry, Chapter 25, Vol. 7; Heinzelman, R., Ed.;<br />
Academic Press: New York, NY, 1972, pp. 269-279.<br />
<strong>Nagasawa</strong>, H. T.; Fraser, P. S.; Yuzon, D. L. A New Method for Nitrosation of Proline<br />
and Related Secondary- -Amino Acids to N-Nitrosamine with Possible Oncogenic<br />
Activity. J. Med. Chem. 1973, 16, 583-585.
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Shirota, F. N. 2-Aminoadamantane-2-carboxylic Acid,<br />
A Rigid, Achiral, Tricyclic - -Amino Acid with Transport Inhibitor Properties. J. Med.<br />
Chem. 1973, 16, 823-826.<br />
Forsyth, G. W.; <strong>Nagasawa</strong>, H. T.; Alexander, C. S. Acetaldehyde Metabolism by the<br />
Rat Heart. Proc. Soc. Exp. Biol. Med. 1973, 144, 498-500.<br />
<strong>Nagasawa</strong>, H. T.; Fullerton, D. S. Reactions of Interest in Medicinal Chemistry. In<br />
Annual Reports in Medicinal Chemistry, Chapter 31, Vol. 8; Heinzelman, R., Ed.;<br />
Academic Press: New York, NY, 1973, pp. 303-312.<br />
<strong>Nagasawa</strong>, H. T.; Shirota, F. N.; Mizuno, N. S. The Mechanisms of Alkylation of DNA<br />
by 5-(3-Methyl-1-triazeno)imidazole-4-carboxamide (MIC), a Metabolite of DIC (NSC-<br />
45388). Non-involvement of Diazomethane. Chem. Biol. Interact. 1974, 8, 403-413.<br />
Thompson, R. D.; <strong>Nagasawa</strong>, H. T.; Jenne, J. W. The Determination of Theophylline<br />
and Its Metabolites in Human Urine and Serum by High Pressure Liquid<br />
Chromatography. J. Lab. Clin. Med. 1974, 84, 584-593.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Shirota, F. N. Potential Latentiation Forms of<br />
Biologically-Active Compounds Based on Action of Leucine Aminopeptidase. Dipeptide<br />
Derivatives of the Tricycloaliphatic -Amino Acid, Adamantanine. J. Med. Chem. 1975,<br />
18, 826-830.<br />
Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Synthesis of Ethambutol- 14 C Dihydrochloride [(+)-N,N'bis(1-Hydroxy-2-butyl)ethylene-U-<br />
14 C-diamine Dihydrochloride]. J. Labelled Compd.<br />
1975, 11, 457-459.<br />
Jenne, J. W.; <strong>Nagasawa</strong>, H. T.; McHugh, R.; MacDonald, F.; Wyse, E. Decreased<br />
Theophylline Half-life in Cigarette Smokers. Life Sci. 1975, 17, 195-198.<br />
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; Constantino, N. V.; Alexander, C. S. Diversion of<br />
Ethanol Metabolism by Sulfhydryl Amino Acids. D-Penicillamine-directed Excretion of<br />
2,5,5-Trimethyl-D-thiazolidine-4-carboxylic Acid in the Urine of Rats after Ethanol<br />
Administration. Life Sci. 1975, 17, 707-713.<br />
<strong>Nagasawa</strong>, H. T.; Kuo, T. H.; Shirota, F. N.; Alexander, C. S. An Intestinal Arylamidase<br />
that Selectively Hydrolyzes Certain Aromatic Amides. Biochem. Pharmacol. 1976, 25,<br />
855-858.<br />
Jenne, J. W.; <strong>Nagasawa</strong>, H. T.; Thompson, R. D. The Relationship of Urine Metabolites<br />
of Theophylline to Serum Theophylline Levels. Clin. Pharmacol. Ther. 1976, 19, 375-<br />
381.
Vince, R.; Almquist, R. G.; Ritter, C. L.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. An Active<br />
Puromycin Analog Derived from a Non-nephrotoxic Aminonucleoside. Life Sci. 1976,<br />
18, 345-350.<br />
Forsyth, G. W.; <strong>Nagasawa</strong>, H. T.; Alexander, C. S. Ethanol Metabolism by the Rat<br />
Heart and Alcohol Dehydrogenase Activity. Can. J. Biochem. 1976, 54, 539-545.<br />
Alexander, C. S.; Forsyth, G. W.; <strong>Nagasawa</strong>, H. T.; Kohloff, J. G. Alcoholic<br />
Cardiomyopathy in Mice. Myocardial Glycogen, Lipids and Certain Enzymes. J. Mol.<br />
Cell. Cardiol. 1977, 9, 235-245.<br />
Alexander, C. S.; Sekhri, K. K.; <strong>Nagasawa</strong>, H. T. Alcoholic Cardiomyopathy in Mice.<br />
Electron Microscopic Observations. J. Mol. Cell. Cardiol. 1977, 9, 247-254.<br />
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; DeMaster, E. G.; Alexander, C. S. Lowering of<br />
Ethanol-Derived Circulating Blood Acetaldehyde in Rats by D-Penicillamine. Life Sci.<br />
1977, 20, 187-194.<br />
Goon, D. J. W.; <strong>Nagasawa</strong>, H. T. A Simple, Rapid and Semi-Automated Method for the<br />
Determination of Expired 14 CO2 in Metabolism Studies with Laboratory Animals. Res.<br />
Commun. Chem. Pathol. Pharmacol. 1977, 16, 745-748.<br />
Shirota, F. N.; <strong>Nagasawa</strong>, H. T.; Elberling, J. A. Potential Inhibitors of Collagen<br />
Biosynthesis. 4,4-Difluoro-L-proline and 4,4-Dimethyl-DL-proline and Their Activation<br />
by Prolyl-t-RNA Ligase. J. Med. Chem. 1977, 20, 1176-1181.<br />
DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Disulfiram-induced Acetonemia in the Rat and Man.<br />
Res. Commun. Chem. Pathol. Pharmacol. 1977, 18, 361-364.<br />
Shirota, F. N.; <strong>Nagasawa</strong>, H. T.; Elberling, J. A. Potential Inhibitors of Collagen<br />
Biosynthesis. 5.5-Difluorolysine and 5.5-Dimethyllysine and Their Activation by Lysyl-t-<br />
RNA Ligase. J. Med. Chem. 1977, 20, 1623-1627.<br />
<strong>Nagasawa</strong>, H. T.; Muldoon, W. P.; Shirota, F. N. Nitramino Acids. Synthesis and<br />
Biological Evaluation of 1-Nitroproline, 1-Nitropipecolic Acid and N-nitrosarcosine. J.<br />
Med. Chem. 1977, 20, 1588-1591.<br />
<strong>Nagasawa</strong>, H. T.; Alexander, C. S.; Goon, D. J. W.; DeMaster, E. G. Lowering of Blood<br />
Acetaldehyde Levels as a Therapeutic Approach to Alcoholism. In Alcohol and<br />
Aldehyde Metabolizing Systems, Vol. III; Thurman, R. G., Williamson, J. R., Pratt, H.,<br />
Chance, B., Eds.; Academic Press: New York, NY, 1978, pp 529-536.<br />
Alexander, C. S.; <strong>Nagasawa</strong>, H. T.; DeMaster, E. G. Lowering of Blood Acetaldehyde<br />
Levels--A Possible Approach to Prevention of Alcoholic Cardiomyopathy. In Recent<br />
Advances in Studies on Cardiac Structures and Metabolism, Vol. 12, Cardiac
Adaptation; Kobayashi, T.; Ito, Y.; Rona, G.; Eds.; University Park Press: Baltimore,<br />
MD, 1978, pp 345-350.<br />
DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Isoprene, an Endogenous Constituent of Human<br />
Alveolar Air with a Diurnal Pattern of Excretion. Life Sci. 1978, 22, 91-98.<br />
DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Inhibition of Aldehyde Dehydrogenase by<br />
Propiolaldehyde, a Possible Metabolite of Pargyline. Res. Commun. Chem. Pathol.<br />
Pharmacol. 1978, 21, 497-505.<br />
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; DeMaster, E. G. 2, 5,5-Trimethylthiazolidine-4carboxylic<br />
Acid, a D(-)-Penicillamine-directed Pseudometabolite of Ethanol.<br />
Detoxication Mechanism for Acetaldehyde. J. Med. Chem. 1978, 21, 1274-1279.<br />
Elberling, J. A.; Zera, R. T.; Magnan, S. D. J.; <strong>Nagasawa</strong>, H. T. Phthaloyl-L-glutamic<br />
Anhydride (2-Phthalimidoglutaric Anhydride). Org. Prep. Proceed. Int. 1979, 11, 67-70.<br />
Shirota, F. N.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Propiolaldehyde, a Pargyline<br />
Metabolite that Irreversibly Inhibits Aldehyde Dehydrogenase. Isolation from a Hepatic<br />
Microsomal System. J. Med. Chem. 1979, 22, 463-464.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; DeMaster, E. G. Structural Requirements for the<br />
Sequestration of Metabolically-generated Acetaldehyde. J. Med. Chem. 1980, 23, 140-<br />
143.<br />
Shirota, F. N.; DeMaster, E. G.; Elberling, J. A.; <strong>Nagasawa</strong>, H. T. Metabolic<br />
Depropargylation and Its Relationship to Aldehyde Dehydrogenase Inhibition In Vivo. J.<br />
Med. Chem. 1980, 23, 669-673.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Shirota, F. N. Latentiated Forms of the Transport<br />
Inhibitory -Amino Acid, Adamantanine. J. Pharm. Sci. 1980, 69, 1022-1025.<br />
DeMaster, E. G.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Microsomal N-Depropargylation of<br />
Pargyline to Propiolaldehyde, an Irreversible Inhibitor of Mitochondrial Aldehyde<br />
Dehyddrogenase. In Alcohol and Aldehyde Metabolizing Systems, Vol. IV; Thurman, R.<br />
G., Ed.; Plenum Press, 1980, pp 219-228.<br />
Zera, R. T.; <strong>Nagasawa</strong>, H. T. N-Acetyl-DL-penicillamine and Acetominophen Toxicity in<br />
Mice. J. Pharm. Sci. 1980, 69, 1005-1006.<br />
Kaplan, E.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Effect of Pargyline on Hepatic<br />
Glutathione Levels in Rats Treated Acutely and Chronically with Ethanol. Res.<br />
Commun. Chem. Pathol. Pharmacol. 1980, 30, 577-580.<br />
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; Shirota, F. N. Epimerization of C-2- of 2-Substituted<br />
Thiazolidine-4-carboxylic Acids. J. Heterocycl. Chem. 1981, 18, 1047-1051.
Zera, R. T.; <strong>Nagasawa</strong>, H. T. Inhibition of Paraldehyde Metabolism in Mice by SKF-<br />
525A. IRCS Med. Sci. 1981, 9, 988.<br />
Zera, R. T.; <strong>Nagasawa</strong>, H. T. Metabolism of Paraldehyde to Acetaldehyde by Rat Liver<br />
Microsome. Res. Commun. Chem. Pathol. Pharmacol. 1981, 34, 531-541.<br />
<strong>Nagasawa</strong>, H. T.; Magnan, S. D. J.; Foltz, R. L. Differentiation of - from -Glutamyl<br />
Dipeptides by Chemical Ionization Mass Spectrometry. Biomed. Mass Spectrom. 1982,<br />
9, 252-256.<br />
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; Zera, R. T.; Yuzon, D. L. Prodrugs of L-Cysteine as<br />
Liver Protective Agents. 2(R,S)-Methylthiazolidine-4(R)-carboxylic Acid, a Latent<br />
Cysteine. J. Med. Chem. 1982, 25, 489-491.<br />
DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Effect of Phenobarbital Treatment on Pargylineinduced<br />
Acetaldehydemia after Ethanol in Sprague-Dawley and Fischer 344 Schf Rats.<br />
Res. Commun. Subs. Abuse 1982, 3, 211-217.<br />
Shirota, F. N.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Studies on the Cyanamide-Ethanol<br />
Interaction. Dimethylcyanamide as an Inhibitor of Aldehyde Dehydrogenase. Biochem.<br />
Pharmacol. 1982, 31, 1999-2004.<br />
Magnan, S. D. J.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Drug Latentiation by gamma-<br />
Glutamyl-transpeptidase. J. Med. Chem. 1982, 25, 1018-1021.<br />
DeMaster, E. G.; Kaplan, E; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Metabolic Activation of<br />
Cyanamide by Liver Mitochondria: A Requirement for the Inhibition of Aldehyde<br />
Dehydrogenase Enzymes. Biochem. Biophys. Res. Commun. 1982, 107, 1333-1339.<br />
DeMaster, E. G.; Sumner, H. W.; Kaplan, E.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Pargylineinduced<br />
Hepatoxicity. Possible Mediation by the Reactive Metabolite, propiolaldehyde.<br />
Toxicol. Appl. Pharmacol. 1982, 65, 390-401.<br />
DeMaster, E. G.; <strong>Nagasawa</strong>, H. T.; Shirota, F. N. Metabolic Activation of Cyanamide to<br />
an Inhibitor of Aldehyde Dehydrogenase In Vitro. Pharmacol. Biochem. Behav. 1983,<br />
18 (Suppl. 1), 273-278.<br />
Halberg, J.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T.; Halberg, F. Individualized and Group<br />
Circadian Rhythm and Corresponding Chronodems for Isoprene in Human Alveolar Air.<br />
Biochimica Clinica, 1983, 7, 905-914.<br />
DeMaster, E. G.; Shirota, F. N.; Redfern, B.; Goon, D. J. W.; <strong>Nagasawa</strong>, H. T. Analysis<br />
of Hepatic Reduced Glutathione, Cysteine, and Homocysteine, by Cation Exchange<br />
High Pressure Liquid Chromotography with Electrochemical Detection. J. Chromatogr.<br />
1984, 308, 83-91.
<strong>Nagasawa</strong>, H. T.; Goon, D. J. W.; Muldoon, W. P.; Zera, R. 2-Substituted Thiazolidine-<br />
4(R)-Carboxylic Acids as Prodrugs of L-Cysteine. Protection of Mice Against<br />
Acetaminophen Hepatotoxicity. J. Med. Chem., 1984, 27, 591-596.<br />
Shirota, F. N.; <strong>Nagasawa</strong>, H. T.; Kwon, C. H.; DeMaster, E. G. N-Acetylcyanamide, the<br />
Major Urinary Metabolite of Cyanamide in Rat, Rabbit, Dog and Man. Drug Metab.<br />
Dispos., 1984, 12, 337-344.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; DeMaster, E. G. Latent Inhibitors of Aldehyde<br />
Dehydrogenase as Alcohol Deterrent Agents. J. Med. Chem. 1984, 27, 1333-1339.<br />
DeMaster, E. G.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. The Metabolic Activation of<br />
Cyanamide to an Inhibitor of Aldehyde Dehydrogenase is Catalyzed by Catalase.<br />
Biochem. Biophys. Res. Commun. 1984, 122, 358-365.<br />
<strong>Nagasawa</strong>, H. T.; Smith, W. E.; Kwon, C. H.; Goon, D. J. W. Acetylative Cleavage of<br />
Arylsulfonylureas to N-Acetylarylsulfonamides and Isocyanates. J. Org. Chem. 1985,<br />
50, 4993-4996.<br />
DeMaster, E. G.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Catalase Mediated Conversion of<br />
Cyanamide to an Inhibitor of Aldehyde Dehydrogenase. Alcohol 1985, 2, 117-121.<br />
<strong>Nagasawa</strong>, H. T.; DeMaster, E. G.; Kwon, C. H.; Fraser, P. S.; Shirota, F. N. Structure<br />
vs. Activity in the Sulfonylurea-Mediated Disulfiram-Ethanol Reaction. Alcohol 1985, 2,<br />
123-128.<br />
<strong>Nagasawa</strong>, H. T.; Kwon, C. H.; DeMaster, E. G.; Shirota, F. N. Prodrugs of Cyanamide<br />
as Long-Acting Alcohol Deterrent Agents. Biochem. Pharmacol. 1986, 35, 129-132.<br />
DeMaster, E. G.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. The Role of Propiolaldehyde and<br />
Other Metabolites in the Pargyline Inhibition of Rat Liver Aldehyde Dehydrogenase.<br />
Biochem. Pharmacol. 1986, 35, 1481-1489.<br />
DeMaster, E. G.; Redfern, B.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Differential Inhibition of<br />
Rat Tissue Catalase by Cyanamide. Biochem. Pharmacol. 1986, 35, 2081-2085.<br />
Kwon, C. H.; <strong>Nagasawa</strong>, H. T.; DeMaster, E. G.; Shirota, F. N. Acyl, N-Protected alphaaminoacyl,<br />
and peptidyl Derivatives as Prodrug Forms of the Alcohol Deterrent Agent,<br />
Cyanamide. J. Med. Chem. 1986, 29, 1922-1929.<br />
Shirota, F. N.; DeMaster, E. G.; Kwon, C. H.; <strong>Nagasawa</strong>, H. T. Metabolism of<br />
cyanamide to cyanide and an inhibitor of aldehyde dehydrogenase (AlDH) by rat liver<br />
microsomes. Alcohol Alcoholism 1987, Suppl. 1, 219-223.
Shirota, F. N.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Cyanide is a Product of the Catalase<br />
Mediated Oxidation of the Alcohol Deterrent Agent, Cyanamide. Toxicol. Lett. 1987, 37,<br />
7-12.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Roberts, J. C. ß-Substituted Cysteines as<br />
Sequestering Agents for Ethanol-Derived Acetaldehyde In Vivo. J. Med. Chem. 1987,<br />
30, 1373-1378.<br />
Kwon, C.-H.; <strong>Nagasawa</strong>, H. T. Facile Synthesis of 2-Iminohydantoins. Synth.<br />
Commun. 1987, 17, 1677-1682.<br />
Roberts, J. C.; <strong>Nagasawa</strong>, H. T.; Zera, R. T.; Fricke, R. F.; Goon, D. J. W. Prodrugs of<br />
L-cysteine as protective agents against acetaminophen-induced hepatotoxicity. 2-<br />
(Polyhydroxyalky)- and 2-(Polyacetoxyalkyl)-Thiazolidine-4(R)-Carboxylic Acids. J.<br />
Med. Chem. 1987, 30, 1891-1896.<br />
DeMaster, E. G.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Oxidation of cyanamide by a cumene<br />
hydroperoxide supported catalase reaction yields cyanide and an inhibitor of aldehyde<br />
dehydrogenase. Biochem. Arch. 1988, 4, 203-207.<br />
Zieve, L.; <strong>Nagasawa</strong>, H. T. Methanethiols and Derivatives in Hepatic Failure. J. Lab.<br />
Clin. Med. 1988, 11 (Editorial), 595-597.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Shirota, F. N.; DeMaster, E. G. A nonhypoglycemic<br />
Chlorpropamide Analog that Inhibits Aldehyde Dehydrogenase. Alcoholism Clin. Exp.<br />
Res. 1988, 12, 563-565.
Shirota, F. N.; <strong>Nagasawa</strong>, H. T. The Metabolism of the Alcohol Deterrent Agent,<br />
Cyanamide, is Not Altered by Acetylator Phenotype. In Biomedical and Social Aspects<br />
of Alcoholism; Kuriyama, K., Takada, A., Ishii, H., Eds.; Excerpta Medica: New York,<br />
1988; pp 127-130.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; DeMaster, E. G.; Shirota, F. N. N 1 -Alkyl Substituted<br />
Derivatives of Chlorprropamide as Inhibitors of Aldehyde Dehydrogenase. J. Med.<br />
Chem. 1989, 32, 1335-1340.<br />
Clark, C.; Woodson, M. M.; Winge, V. B.; <strong>Nagasawa</strong>, H. T. The Antiviral Drug<br />
Amantadine Has a Direct Inhibitory Effect on T-Lymphocytes. Immunopharmacol. 1989,<br />
18, 195-204.<br />
Goon, D. J. W.; Elberling, J. A.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Synthesis of [ 14 C]-<br />
Pargyline {N-Methyl-N-([1- 14 C]-propargyl)-benzylamine} with the Radioactive Label on<br />
the Propargyl Group. J. Label. Comp. Radiopharmaceuticals 1990, 18, 195-204.<br />
Kwon, C.-H.; <strong>Nagasawa</strong>, H. T. Intermolecular Decomposition Reactions of N-<br />
Acylcyanamides. J. Org. Chem. 1990, 55, 3403-3406.<br />
<strong>Nagasawa</strong>, H. T.; DeMaster, E. G.; Redfern, B.; Shirota, F. N.; Goon, D. J. W. Evidence<br />
for Nitroxyl in the Catalase-Mediated Bioactivation of the Alcohol Deterrent Agent,<br />
Cyanamide. J. Med. Chem. 1990, 33, 3120-3122.<br />
Clark, C.; Woodson, M. M.; <strong>Nagasawa</strong>, H. T. Inhibition of Lymphocyte Proliferation by<br />
Amantadine and its Isomer, 2-Aminoadamantane, Impact on Lyt-2+ T-cells while<br />
Sparing L3T4+ T-cells. Immunopharmacol. 1991, 21, 41-50.<br />
<strong>Nagasawa</strong>, H. T.; Kwon, C.-H.; Elberling, J. A.; Lee, M. J. C.; DeMaster, E. G.; Shirota,<br />
F. N.; Goon, D. J. W. Rationale for Alcoholism Treatment Based on Inhibition of<br />
Aldehyde Dehydrogenase. In Novel Pharmacological Interventions for Alcoholism;<br />
Naranjo, C.A., Sellers, E.M., Eds.; Springer-Verlag: New York, 1991, pp 247-250.<br />
Shirota, F. N.; Elberling, J. A.; <strong>Nagasawa</strong>, H. T.; DeMaster, E. G. Failure of Glutathione<br />
and Cysteine Prodrugs to Block the Chlorpropamide Induced Inhibition of Aldehyde<br />
Dehydrogenase in Rats. Biochem. Pharmacol. 1992, 43, 916-918.<br />
Roberts, J. C.; Charyulu, R. L.; Zera, R. T.; <strong>Nagasawa</strong>, H. T. Protection Against<br />
Acetaminophen Hepatotoxicity by the Glutathione Elevating Agent, Ribose-Cysteine<br />
(RibCys). Pharmacol. Toxicol. 1992, 70, 281-285.<br />
<strong>Nagasawa</strong>, H. T.; Lee, M. J. C.; Kwon, C.-H.; Shirota, F. N.; DeMaster, E. G. An N-<br />
Hydroxylated Derivative of Cyanamide that Inhibits Aldehyde Dehydrogenase. Alcohol<br />
1992, 9, 349-353.
Lee, M. J. C.; Elberling, J. A.; <strong>Nagasawa</strong>, H. T. N 1 -Hydroxylated Derivatives of<br />
Chlorpropamide and Its Analogs as Inhibitors of Aldehyde Dehydrogenase In Vivo. J.<br />
Med. Chem. 1992, 35, 3641-3647.<br />
Lee, M. J. C.; <strong>Nagasawa</strong>, H. T.; Elberling, J. A.; DeMaster, E. G. Prodrugs of Nitroxyl<br />
as Inhibitors of Aldehyde Dehydrogenase. J. Med. Chem. 1992, 35, 3648-3652.<br />
DeMaster, E.G.; Shirota, F.N.; <strong>Nagasawa</strong>, H.T. A Beckmann Type Dehydration of n-<br />
Butyaldoxime Catalyzed by Cytochrome P-450. J. Org. Chem. 1992, 57, 6074-6075.<br />
Fukuto, J. M.; Hszieh, R.; Gulati, P.; Chiang, K. T.; <strong>Nagasawa</strong>, H. T. N,O-Diacylated-Nhydroxyarylsulfonamides:<br />
Nitroxyl Precursors With Potent Smooth Muscle Relaxant<br />
Properties. Biochem. Biophys. Res. Commun. 1992, 137, 1367-1373.<br />
DeMaster, E. G.; Redfern, B.; Shirota, F. N.; Crankshaw, D. L.; <strong>Nagasawa</strong>, H. T.<br />
Metabolic Activation of n-Butyraldoxime by Rat Liver Microsomal Cytochrome P-450: A<br />
Requirement for the Inhibition of Aldehyde Dehydroqenase. Biochem. Pharmacol. 1993,<br />
46, 117-123.<br />
<strong>Nagasawa</strong>, H. T.; Yost, Y.; Elberling, J. A.; Shirota, F. N.; DeMaster, E. G. Nitroxyl<br />
Analogs as Inhibitors of Aldehyde Dehydrogenase. C-Nitroso Compounds. Biochem.<br />
Pharmacol. 1993, 45, 2129-2134.<br />
Fukuto, T. M.; Gulati, P.; <strong>Nagasawa</strong>, H. T. Involvement of Nitroxyl (HNO) in the<br />
Cyanamide-Induced Vasorelaxation of Rabbit Aorta. Biochem. Pharmacol. 1994, 47,<br />
922-924.<br />
Goon, D. J. W.; <strong>Nagasawa</strong>, H. T.; Keyler, D. E.; Ross, C. A.; Pentel, P. R. The<br />
Glutaramyl-β-Alanyl Spacer Group for Haptenic Coupling to Proteins. Preparation of<br />
Immunogens for Antibody Production Against Polychlorinated Biphenyls. Bioconj.<br />
Chem. 1994, 5, 418-422.<br />
Keyler, D. E.; Goon, D. J. W.; Shelver, W. L.; Ross, C. A.; <strong>Nagasawa</strong>, H. T.; Pentel, P.<br />
R. Redistribution and Enhanced Urinary Excretion of 2,2'4,4'5,5'-Hexachlorobiphenyl<br />
(HCB) in Rats Using HCB-Specific IgGand Fab Fragments. Biochem. Pharmacol. 1994,<br />
48, 767-773.<br />
<strong>Nagasawa</strong>, H. T.; Elberling, J. A.; Goon, D. J. W.; Shirota, F. N. Latent Isocyanates as<br />
Inhibitors of Aldehyde Dehydrogenase In Vivo. J. Med. Chem. 1994, 37, 422-426.<br />
<strong>Nagasawa</strong>, H. T.; Kawle, S. P.; Elberling, J. A.; DeMaster, E. G.; Fukuto, J. M.<br />
Prodrugs of Nitroxyl as Potential Aldehyde Dehydrogenase Inhibitors vis-a-vis Vascular<br />
Smooth Muscle Relaxants. J. Med. Chem. 1995, 38, 1865-1871.<br />
<strong>Nagasawa</strong>, H. T.; DeMaster, E. G.; Goon, D. J. W.; Kawle, S. P.; Shirota, F. N.<br />
Carbethoxylating Agents as Inhibitors of Aldehyde Dehydrogenase. J. Med. Chem.<br />
1995, 38, 1872-1876.
DeMaster, E. G.; Quast, B. J.; Redfern, B.; <strong>Nagasawa</strong>, H. T. Reaction of Nitric Oxide<br />
with the Free Sulfhydryl Group of Human Serum Albumin Yields a Sulfenic Acid and<br />
Nitrous Oxide. Biochemistry 1995, 34, 11494-11499.<br />
<strong>Nagasawa</strong>, H. T.; Cohen, J. F.; Rathbun, W. B. Mixed Disulfides of L-Cysteine and its<br />
Derivatives with 2-Mercapoethanol. Org. Prep. Proc. Int. 1996, 28, 237-241.<br />
Rathbun, W. B.; Killen, C. E.; Holleschau, A. M.; <strong>Nagasawa</strong>, H. T. Maintenance of<br />
Hepatic Homeostasis and Prevention of Acetaminophen-lnduced Cataracts in Mice by<br />
L-Cysteine Prodrugs. Biochem. Pharmacol. 1996, 51, 1111-1116.<br />
<strong>Nagasawa</strong>, H. T.; Cohen, J. F.; Holleschau, A. M.; Rathbun, W. B. Augmentation of<br />
Human and Rat Lenticular Glutathione in Vitro by Prodrugs of y-Glutamyl-L-Cysteine. J.<br />
Med. Chem. 1996, 39, 1676-1681.<br />
Rathbun, W. B.; Holleschau, A. M.; Cohen, J. F.; <strong>Nagasawa</strong>, H. T. Prevention of<br />
Acetaminophen and Naphthalene-Induced Cataract and Glutathione Loss by CYSSME.<br />
Invest. Ophthol. Vis. Sci. 1996, 37, 923-929.<br />
Rathbun, W. B.; <strong>Nagasawa</strong>, H. T.; Killen, C. E. Prevention of Naphthalene-Induced<br />
Cataract and Hepatic Glutathione Loss by the L-Cysteine Prodrugs, MTCA and PICA.<br />
Exp. Eye Res. 1996, 62, 433-441.<br />
Holleschau, A. M.; Rathbun, W. B.; <strong>Nagasawa</strong>, H. T. An HPLC Radiotracer Method for<br />
Assessing the Ability of L-Cysteine Prodrugs to Maintain Glutathione Levels in Cultured<br />
Rat Lens. Curr. Eye Res. 1996, 15, 501-510.<br />
Shirota, F. N.; Goon, D. J. W.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Nitrosyl Cyanide, a<br />
Putative Metabolic Oxidation Product of the Alcohol-Deterrent Agent Cyanamide.<br />
Biochem. Pharmacol. 1996, 52, 141-147.<br />
<strong>Nagasawa</strong>, H. T.; Shoeman, D. W.; Cohen, J. F.; Rathbun, W. B. Protection against<br />
Acetaminophen-lnduced Hepatotoxicity by L-CySSME and its N-Acetyl and Ethyl Ester<br />
Derivatives. J. Biochem. Toxicol. 1996, 11, 289-295.<br />
Shirota, F. N.; Stevens-Johnk, J. M.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Novel Prodrugs<br />
of Cyanamide that Inhibit Aldehyde Dehydrogenase in Vivo. J. Med. Chem. 1997, 40,<br />
1870.<br />
DeMaster, E. G.; Redfern, B.; Quasi, B. J.; Dahlseid, T.; <strong>Nagasawa</strong>, H. T. Mechanism<br />
for the Inhibition of Aldehyde Dehydrogenase by Nitric Oxide. Alcohol 1997, 14, 181-<br />
189.<br />
Deveraj, V. R.; Sreerama, L.; Lee, M. J. C.; <strong>Nagasawa</strong>, H. T., Sladek, N .E. Yeast<br />
Aldehyde Dehydrogenase Sensitivity to Inhibition by Chlorpropamide Analogues as an<br />
Indicator of Human Aldehyde Dehydrogenase to these Agents. Adv. Exp. Med. Biol.<br />
1997, 40, 1870-1875.
Bantseev, V.; Bhardwaj, R.; Rathbun, W.; <strong>Nagasawa</strong>, H.; Trevithick, J. R. Antioxidants<br />
and cataract: (cataract inductin in space environment and application to terrestrial aging<br />
cataract). Biochem. Mol. Biol. Int. 1997, 42, 1189-1197.<br />
Rekha, G. K.; Deveraj, V. R.; Sreerama, L.; Lee, M.J . C.; <strong>Nagasawa</strong>, H. T.; Sladek, N.<br />
E. Inhibition of Human Class 3 Aldehyde Dehydrogenase and Sensitization of Tumor<br />
Cells that Express Significant Amounts of this Enzyme to Oxazaphosphorines by<br />
Chlorpropamide Analogs. Biochem. Pharmacol. 1998, 55, 465-474.<br />
DeMaster, E. G.; Redfern, B.; <strong>Nagasawa</strong>, H. T. Mechanism of inhibition of aldehyde<br />
dehydrogenase by nitroxyl, the active metabolite of the alcohol deterrent agent<br />
cyanamide. Biochem. Pharmacol. 1998, 55, 2007-2015.<br />
Shoeman, D. W.; <strong>Nagasawa</strong>, H. T. The reaction of nitroxyl (HNO) with nitrosobenzene<br />
gives cupferon (N-nitrosophenylhydroxylamine). Nitric Oxide 1998, 2, 66-72.<br />
Conway, T. T.; DeMaster, E. G.; Lee, M. J. C.; <strong>Nagasawa</strong>, H. T. Prodrugs of nitroxyl<br />
and nitrosobenzene as cascade latentiated inhibitors of aldehyde dehydrogenase. J.<br />
Med. Chem. 1998, 41, 2903-2909.<br />
Wong, P. S.; Hyun J.; Fukuto, J. M.; Shirota, F. N.; DeMaster, E. G.; Shoeman, D. W.;<br />
<strong>Nagasawa</strong>, H. T. The reaction between S-nitrosothiols and thiols: Generation of nitroxyl<br />
and subsequent chemistry. Biochemistry 1998, 37, 5363-5371.<br />
King, S. B.; <strong>Nagasawa</strong>, H. T. Chemical approaches towards the generation of nitroxyl<br />
(HNO) in "Nitric Oxide". In Methods in Enzymology, vol. 301, part C: Biological and<br />
Antioxidant Activities. Lester Packer, Ed., Academic Press, New York, 1998, pp211-<br />
220.<br />
Wong, P.; Hyun, J.; Fukuto, J.; Shirota, F.; DeMaster, E.; <strong>Nagasawa</strong>, H. Chemical<br />
mechanisms involved in the reaction between S-nitrosothiols and thiols. The Biology of<br />
Nitric Oxide. Part 6,Vo\ 15; Moncada, S.; Toda, N.; Maeda, H.; Higgs, E. A., Eds.;<br />
Portland Press Proc., 1998, p 310.<br />
Conway, T. T.; DeMaster, E. G.; Goon, D. J. W.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T.<br />
Diethyl-carbamoylating/nitroxylating agents as dual action inhibitors of aldehyde<br />
dehydro-genase. A disulfiram-cyanamide merger. J. Med. Chem. 1999, 42, 4016-4020.<br />
<strong>Nagasawa</strong>, H. T.; Shirota, F. N.; DeMaster, E. G . Alcoholism: Aldehyde<br />
Dehydrogenase Inhibitors as Alcohol Deterrent Agents. Biomedical Chemistry/Applying<br />
Chemical Principles to the Understanding andTreatment of Disease 1999, 73-79.<br />
Shirota, F. N.; DeMaster, E. G.; Lee, M. J. C.; <strong>Nagasawa</strong>, H. T. Generation of nitric<br />
oxide (NO) and possibly nitroxyl (HNO), respectively, by nitrosation of sulfohydroxamic<br />
acids and hydroxamic acids. Nitric Oxide 1999, 3, 445-453.
Elberembally, K.; <strong>Nagasawa</strong>, H. T. 2-Alkoxy-1,2-benzothiazin-3(3H)one 1,1-dioxides as<br />
potential prodrugs of nitroxyl. Sulfur Lett. 1999, 23, 57-65.<br />
Shoeman, D. W.; Shirota, F. N.; DeMaster, E. G.; <strong>Nagasawa</strong>, H. T. Reaction of nitroxyl<br />
(HNO), a putative inhibitor of aldehyde dehydrogenase, with N-acetyl-L-cysteine.<br />
Alcohol 2000, 20, 55-59.<br />
Cohen, J. F.; Elberling, J A.; DeMaster, E. G.; Lin, R. C.; <strong>Nagasawa</strong>, H. T. N-Terminal<br />
dipeptides of D(-)-Penicillamine as sequestration agents for acetaldehyde. J. Med,<br />
Chem. 2000, 43, 1029-1033.<br />
Chen, T. S.; Richie Jr., J. P.; <strong>Nagasawa</strong>, H. T.; Lang, C. A. Glutathione monoethyl ester<br />
protects against glutathione deficiencies due to aging and acetaminophen in mice.<br />
Mechanisms of Ageing and Development 2000, 120, 127-139.<br />
Sladek, N. E.; Rekha, G. K.; Lee, M. J. C.; <strong>Nagasawa</strong>, H. T. Inhibition of ALDH3A1catalyzed<br />
oxidation by chlorpropamide analogues. Chem-Biol. Int. 2001, 130-132, 135-<br />
149.<br />
Srinivasan, C.; Williams, W. M.; <strong>Nagasawa</strong>, H. T.; Chen, T. S. Effects of 2(RS)-npropylthiazolidine-4(R)-carboxylic<br />
acid on extrahepatic sulfhydryl levels in mice treated<br />
with acetaminophen. Biochem. Pharmacol. 2001, 61, 925-931.<br />
Lee, M. J. C.; Shoeman, D. W.; Goon, D. J. W.; <strong>Nagasawa</strong>, H. T. N-<br />
Hydroxybenzenecarboximidic acid derivatives: A new class of nitroxyl-generating<br />
prodrugs. Nitric Oxide 2001, 5, 278-287.<br />
Shirota, F. N.; DeMaster, E. G.; Shoeman, D. W.; <strong>Nagasawa</strong>, H. T. Acetaminopheninduced<br />
suppression of hepatic AdoMet synthetase is attenuated by prodrugs of Lcysteine.<br />
Toxicol. Lett. 2002, 132, 1-8.<br />
Crankshaw, D. L.; Berkeley, L. I.; Cohen, J. F.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T. Doubleprodrugs<br />
of L-cysteine: Differential protection against acetaminophen-induced<br />
hepatotoxicity in mice. J. Biochem. Mol. Toxicol. 2002, 16, 235-244.<br />
Berkeley, L. I.; Cohen, J. F.; Crankshaw, D. L.; Shirota, F. N.; <strong>Nagasawa</strong>, H. T.<br />
Hepatoprotection by L-cysteine-glutathione mixed disulfide, a sulfhydryl-modified<br />
prodrug of glutathione. J. Biochem. Mol. Toxicol. 2003, 17, 95-97.<br />
Fu, X.; Chen, T. S.; Ray, M. B.; <strong>Nagasawa</strong>, H. T.; Williams, W. M. p-Aminphenolinduced<br />
hepatotoxicity in hamsters: role of glutathione. J. Biochem. Mol. Toxicol. 2004,<br />
18, 154-161.<br />
<strong>Nagasawa</strong>, H. T.; Cummings, S. E.; Baskin, S. I. The structure of "ITCA", a urinary<br />
metabolite of cyanide. Prep. Proced. Internat. Briefs 2004, 36, 178-182.
Oz, H. S.; McClain, C. J.; Ray, M. B.; <strong>Nagasawa</strong>, H. T.; Chen, T. S. Diverse antioxidants<br />
protect against acetaminophen heptotoxicity. J. Biochem. Mol. Toxicol. 2004, 18, 361-<br />
368.<br />
Phimister, A. J.; <strong>Nagasawa</strong>, H. T.; Buckpitt, A. R.; Plopper, C. G. Prevention of<br />
naphthalene induced pulmonary toxicity by glutathione prodrugs: roles for glutathione<br />
depletion in adduct formation and cell injury. J. Biochem. Mol. Toxicol. 2005, 19,, 42-52.<br />
Miranda, K. M.; <strong>Nagasawa</strong>, H. T.; Toscano, J. P. Donors of HNO. Current Topics in<br />
Medicinal Chemistry 2005, 5, 649-664.<br />
Oz, H.S.; Chen, C. S.; <strong>Nagasawa</strong>, H. T. Comparative efficacies of two cysteine<br />
prodrugs and a glutathione delivery agent in a colitis model. Translational Res. 2007,<br />
150, 122-129.
Patents:<br />
<strong>Nagasawa</strong>, H. T.; Kwon, C.-H. Acylated Cyanamide Composition for Treating Ethanol<br />
Ingestion. United States Patent No. 4,726,941, Feb. 23, 1988.<br />
<strong>Nagasawa</strong>, H. T.; Roberts, J. C. Method for Elevating Glutathione Levels. U.S. Patent<br />
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